Development of Industrial Robot System with 5th Generation Mobile Communication System

We experimented the configuration of a 5th generation mobile communication system (5G) for factory automation (FA) systems consisting of industrial robots and three-dimensional measurement sensors. We examined the configuration of system components, on the basis of which we developed an experimental FA system. In addition, we confirmed that the need for communication lines is eliminated as 5G greatly reduces the production preparation time when rearranging the parts of the developed FA system.19th International Conference on Control, Automation and Systems (ICCAS 2019), October 15-18, 2019, Jeju, Kore

Methamphetamine increases locomotion and dopamine transporter activity in dopamine d5 receptor-deficient mice.

Dopamine regulates the psychomotor stimulant activities of amphetamine-like substances in the brain. The effects of dopamine are mediated through five known dopamine receptor subtypes in mammals. The functional relevance of D5 dopamine receptors in the central nervous system is not well understood. To determine the functional relevance of D5 dopamine receptors, we created D5 dopamine receptor-deficient mice and then used these mice to assess the roles of D5 dopamine receptors in the behavioral response to methamphetamine. Interestingly, D5 dopamine receptor-deficient mice displayed increased ambulation in response to methamphetamine. Furthermore, dopamine transporter threonine phosphorylation levels, which regulate amphetamine-induced dopamine release, were elevated in D5 dopamine receptor-deficient mice. The increase in methamphetamine-induced locomotor activity was eliminated by pretreatment with the dopamine transporter blocker GBR12909. Taken together, these results suggest that dopamine transporter activity and threonine phosphorylation levels are regulated by D5 dopamine receptors

Kaempferol Has Potent Protective and Antifibrillogenic Effects for α-Synuclein Neurotoxicity In Vitro

Aggregation of α-synuclein (α-Syn) is implicated in the pathogenesis of Parkinson’s disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA). Therefore, the removal of α-Syn aggregation could lead to the development of many new therapeutic agents for neurodegenerative diseases. In the present study, we succeeded in generating a new α-Syn stably expressing cell line using a piggyBac transposon system to investigate the neuroprotective effect of the flavonoid kaempferol on α-Syn toxicity. We found that kaempferol provided significant protection against α-Syn-related neurotoxicity. Furthermore, kaempferol induced autophagy through an increase in the biogenesis of lysosomes by inducing the expression of transcription factor EB and reducing the accumulation of α-Syn; thus, kaempferol prevented neuronal cell death. Moreover, kaempferol directly blocked the amyloid fibril formation of α-Syn. These results support the therapeutic potential of kaempferol in diseases such as synucleinopathies that are characterized by α-Syn aggregates

DAT levels in D5R-KO mice.

<p>(a) Dialysate samples were collected at a sampling rate of 2 µl/min for 20 min during a baseline period of 60 min and then for an experimental period of 120 min following the METH challenge (2.5 mg/kg; arrow). The interaction between blocker pretreatment and challenge was F_(5,120) = 6.66 and <i>p</i><0.0001. The simple main effects of blocker pretreatment at 40 and 60 min were F_(1,144) = 13.50 and 8.08, and <i>p</i><0.001 and <i>p</i><0.001, respectively. WT, filled circles (<i>n</i> = 7); D5R-KO, open circles (<i>n</i> = 7). DA, dopamine. The arrowhead indicates the time point of saline injection. (b) Dialysate samples were collected for 20 min during a baseline period of 60 min. The animals were then injected with GBR12909 (5 mg/kg) and samples were collected for 20 min during a pretreatment period of 80 min. The animals were then challenged with METH (2.5 mg/kg) and samples were collected throughout the 120 min experimental period. The arrowhead indicates the GBR12909 pretreatment and the arrow indicates the METH challenge. (c) Representative microdialysis probe placements. Dashed lines denote the boundaries of the NA and the anterior commissure (ac). (d) Schematic representations of probe placements in the experimental groups for microdialysis of the NA at two different rostrocaudal levels (1.1 mm and 0.8 mm rostral from the bregma). The short lines indicate the probe tracks. Seven WT and seven D5R-KO probe track cases are overlaid on representative sections.</p

DAT phosphorylation in D5R-KO mice.

<p>DAT proteins were immunoprecipitated from whole brain lysates and were then immunoblotted with anti-phosphothreonine and anti-DAT antibodies. (a) Upper: Representative Western blot of phospho-threonine signals of immunoprecipitated DAT proteins. Lower: Total DAT protein levels of the same samples. WT, D5R-KO, and DAT-KO genotypes are indicated. (b) The intensities of the phosphothreonine bands were normalized to the intensities of the total DAT protein bands to quantify the phosphothreonine levels. WT mice, black bar; D5R-KO mice, gray bar. Threonine phosphorylation levels were significantly increased in D5R-KO mouse brains relative to WT mouse brains. Paired t-test: <i>t</i> = −2.59 and <i>p</i><0.05. * <i>p</i><0.05.</p

Generation of D5R-KO mice.

<p>(a) Design of the D5R gene targeting vector. Upper diagram: restriction enzyme map for the WT D5R gene locus. The black part of the box corresponds to the D5R gene coding region and the white part of the box represents the noncoding region. Middle diagram: the D5R gene targeting vector. Lower diagram: the D5R gene locus in the D5R-KO mice. Bottom diagram: Probes used for recombinant ES cell screening are indicated. (b) Genomic Southern blotting with a 3′ region probe. Genomic DNA was collected from WT (+/+), heterogeneous (+/−), and homogenous (−/−) D5R mice and subjected to electrophoresis and Southern blotting. The bands corresponding to wild-type and mutant DNA are indicated. (c) mRNA was collected from WT (+/+), heterogeneous (+/−), and homogenous (−/−) animals and subjected to electrophoresis and Northern blotting with a D5R cDNA probe. D5R mRNA was absent from the homogenous (−/−) D5R-KO animals.</p

Development of Industrial Robot System with 5th Generation Mobile Communication System

We experimented the configuration of a 5th generation mobile communication system (5G) for factory automation (FA) systems consisting of industrial robots and three-dimensional measurement sensors. We examined the configuration of system components, on the basis of which we developed an experimental FA system. In addition, we confirmed that the need for communication lines is eliminated as 5G greatly reduces the production preparation time when rearranging the parts of the developed FA system.19th International Conference on Control, Automation and Systems (ICCAS 2019), October 15-18, 2019, Jeju, Kore

Analysis of Outcomes in Ischemic vs Nonischemic Cardiomyopathy in Patients With Atrial Fibrillation A Report From the GARFIELD-AF Registry

IMPORTANCE Congestive heart failure (CHF) is commonly associated with nonvalvular atrial fibrillation (AF), and their combination may affect treatment strategies and outcomes