56 research outputs found
The Expanded Central Dogma:Genome Resynthesis, Orthogonal Biosystems, Synthetic Genetics
Synthetic biology seeks to probe fundamental aspects of biological form and function by construction [i.e., (re)synthesis] rather than deconstruction (analysis). In this sense, biological sciences now follow the lead given by the chemical sciences. Synthesis can complement analytic studies but also allows novel approaches to answering fundamental biological questions and opens up vast opportunities for the exploitation of biological processes to provide solutions for global problems. In this review, we explore aspects of this synthesis paradigm as applied to the chemistry and function of nucleic acids in biological systems and beyond, specifically, in genome resynthesis, synthetic genetics (i.e., the expansion of the genetic alphabet, of the genetic code, and of the chemical make-up of genetic systems), and the elaboration of orthogonal biosystems and components.</p
A synthetic genetic polymer with an uncharged backbone chemistry based on alkyl phosphonate nucleic acids
The physicochemical properties of nucleic acids are dominated by their highly charged
phosphodiester backbone chemistry. The polyelectrolyte structure decouples information
content (base sequence) from bulk properties such as solubility and has been proposed as a
defining trait of all informational polymers. However, this conjecture has not been tested
experimentally. Here, we describe the encoded synthesis of a genetic polymer with an
uncharged backbone chemistry: alkyl-phosphonate nucleic acids (phNA), in which the
canonical, negatively charged phosphodiester is replaced by an uncharged P-alkylphosphonodiester
backbone. Using synthetic chemistry and polymerase engineering, we
describe the enzymatic, DNA-templated synthesis of P-methyl- and P-ethyl-phNAs, and the
directed evolution of specific streptavidin-binding phNA aptamer ligands directly from
random-sequence, mixed P-methyl- / P-ethyl-phNA repertoires. Our results establish a first
example of the DNA-templated enzymatic synthesis and evolution of an uncharged genetic
polymer and provide a foundational methodology for their exploration as a source of novel,
functional molecules
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Structural Studies of HNA Substrate Specificity in Mutants of an Archaeal DNA Polymerase Obtained by Directed Evolution.
Archaeal DNA polymerases from the B-family (polB) have found essential applications in biotechnology. In addition, some of their variants can accept a wide range of modified nucleotides or xenobiotic nucleotides, such as 1,5-anhydrohexitol nucleic acid (HNA), which has the unique ability to selectively cross-pair with DNA and RNA. This capacity is essential to allow the transmission of information between different chemistries of nucleic acid molecules. Variants of the archaeal polymerase from Thermococcus gorgonarius, TgoT, that can either generate HNA from DNA (TgoT_6G12) or DNA from HNA (TgoT_RT521) have been previously identified. To understand how DNA and HNA are recognized and selected by these two laboratory-evolved polymerases, we report six X-ray structures of these variants, as well as an in silico model of a ternary complex with HNA. Structural comparisons of the apo form of TgoT_6G12 together with its binary and ternary complexes with a DNA duplex highlight an ensemble of interactions and conformational changes required to promote DNA or HNA synthesis. MD simulations of the ternary complex suggest that the HNA-DNA hybrid duplex remains stable in the A-DNA helical form and help explain the presence of mutations in regions that would normally not be in contact with the DNA if it were not in the A-helical form. One complex with two incorporated HNA nucleotides is surprisingly found in a one nucleotide-backtracked form, which is new for a DNA polymerase. This information can be used for engineering a new generation of more efficient HNA polymerase variants
Catalysts from synthetic genetic polymers
The emergence of catalysis in early genetic polymers like RNA is considered a key transition in the origin of life1, predating the appearance of protein enzymes. DNA also demonstrates the capacity to fold into three-dimensional structures and form catalysts in vitro2. However, to what degree these natural biopolymers comprise functionally privileged chemical scaffolds3 for folding or the evolution of catalysis is not known. The ability of synthetic genetic polymers (XNAs) with alternative backbone chemistries not found in nature to fold into defined structures and bind ligands4 raises the possibility that these too might be capable of forming catalysts (XNAzymes). Here we report the discovery of such XNAzymes, elaborated in four different chemistries (ANA (arabino nucleic acids)5, FANA (2′-fluoroarabino nucleic acids)6, HNA (hexitol nucleic acids) and CeNA (cyclohexene nucleic acids)7 directly from random XNA oligomer pools, exhibiting in trans RNA endonuclease and ligase activities. We also describe an XNA-XNA ligase metalloenzyme in the FANA framework, establishing catalysis in an entirely synthetic system and enabling the synthesis of FANA oligomers and an active RNA endonuclease FANAzyme from its constituent parts. These results extend catalysis beyond biopolymers and establish technologies for the discovery of catalysts in a wide range of polymer scaffolds not found in nature8. Evolution of catalysis independent of any natural polymer has implications for the definition of chemical boundary conditions for the emergence of life on earth and elsewhere in the universe9
Reversible Fluorescence Photoswitching in DNA
[Image: see text] We describe the engineering of reversible fluorescence photoswitching in DNA with high-density substitution, and its applications in advanced fluorescence microscopy methods. High-density labeling of DNA with cyanine dyes can be achieved by polymerase chain reaction using a modified DNA polymerase that has been evolved to efficiently incorporate Cy3- and Cy5-labeled cytosine base analogues into double-stranded DNA. The resulting biopolymer, “CyDNA”, displays hundreds of fluorophores per DNA strand and is strongly colored and highly fluorescent, although previous observations suggest that fluorescence quenching at such high density might be a concern, especially for Cy5. Herein, we first investigate the mechanisms of fluorescence quenching in CyDNA and we suggest that two different mechanisms, aggregate formation and resonance energy transfer, are responsible for fluorescence quenching at high labeling densities. Moreover, we have been able to re-engineer CyDNA into a reversible fluorescence photoswitchable biopolymer by using the properties of the Cy3–Cy5 pair. This novel biopolymer constitutes a new class of photoactive DNA-based nanomaterial and is of great interest for advanced microscopy applications. We show that reversible fluorescence photoswitching in CyDNA can be exploited in optical lock-in detection imaging. It also lays the foundations for improved and sequence-specific super-resolution fluorescence microscopy of DNA
Introduction to the themed collection on XNA xeno-nucleic acids.
Dennis Bong, Philip Holliger, and Chaoyong Yang introduce the RSC Chemical Biology themed collection on XNA xeno-nucleic acids
Hydrophobic-cationic peptides modulate RNA polymerase ribozyme activity by accretion.
Accretion and the resulting increase in local concentration is a widespread mechanism in biology to enhance biomolecular functions (for example, in liquid-liquid demixing phases). Such macromolecular aggregation phases (e.g., coacervates, amyloids) may also have played a role in the origin of life. Here, we report that a hydrophobic-cationic RNA binding peptide selected by phage display (P43: AKKVWIIMGGS) forms insoluble amyloid-containing aggregates, which reversibly accrete RNA on their surfaces in an RNA-length and Mg2+-concentration dependent manner. The aggregates formed by P43 or its sequence-simplified version (K2V6: KKVVVVVV) inhibited RNA polymerase ribozyme (RPR) activity at 25 mM MgCl2, while enhancing it significantly at 400 mM MgCl2. Our work shows that such hydrophobic-cationic peptide aggregates can reversibly concentrate RNA and enhance the RPR activity, and suggests that they could have aided the emergence and evolution of longer and functional RNAs in the fluctuating environments of the prebiotic earth
Non-canonical 3′-5′ Extension of RNA with Prebiotically Plausible Ribonucleoside 2′,3′-Cyclic Phosphates
Ribonucleoside
2′,3′-cyclic phosphates (N>p’s)
are generated by multiple prebiotically plausible processes and are
credible building blocks for the assembly of early RNA oligomers.
While N>p’s can be polymerized into short RNAs by non-enzymatic
processes with variable efficiency and regioselectivity, no enzymatic
route for RNA synthesis had been described. Here we report such a
non-canonical 3′-5′ nucleotidyl transferase activity.
We engineered a variant of the hairpin ribozyme to catalyze addition
of all four N>p’s (2′,3′-cyclic A-, G-, U-,
and
CMP) to the 5′-hydroxyl termini of RNA strands with 5′
nucleotide addition enhanced in all cases by eutectic ice phase formation
at −7 °C. We also observed 5′ addition of 2′,3′-cyclic
phosphate-activated β-nicotinamide adenine dinucleotide (NAD>p)
and ACA>p RNA trinucleotide, and multiple additions of GUCCA>p
RNA
pentamers. Our results establish a new mode of RNA 3′-5′
extension with implications for RNA oligomer synthesis from prebiotic
nucleotide pools
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