A Computational Tool for Testing Dose-related Trend Using an Age-adjusted Bootstrap-based Poly-k Test

A computational tool for testing for a dose-related trend and/or a pairwise difference in the incidence of an occult tumor via an age-adjusted bootstrap-based poly-k test and the original poly-k test is presented in this paper. The poly-k test (Bailer and Portier 1988) is a survival-adjusted Cochran-Armitage test, which achieves robustness to effects of differential mortality across dose groups. The original poly-k test is asymptotically standard normal under the null hypothesis. However, the asymptotic normality is not valid if there is a deviation from the tumor onset distribution that is assumed in this test. Our age-adjusted bootstrap-based poly-k test assesses the significance of assumed asymptotic normal tests and investigates an empirical distribution of the original poly-k test statistic using an age-adjusted bootstrap method. A tumor of interest is an occult tumor for which the time to onset is not directly observable. Since most of the animal carcinogenicity studies are designed with a single terminal sacrifice, the present tool is applicable to rodent tumorigenicity assays that have a single terminal sacrifice. The present tool takes input information simply from a user screen and reports testing results back to the screen through a user-interface. The computational tool is implemented in C/C++ and is applied to analyze a real data set as an example. Our tool enables the FDA and the pharmaceutical industry to implement a statistical analysis of tumorigenicity data from animal bioassays via our age-adjusted bootstrap-based poly-k test and the original poly-k test which has been adopted by the National Toxicology Program as its standard statistical test

Quasi-Static Analysis on Transoral Surgical Tendon-Driven Articulated Robot Units

Wire actuation in tendon-driven continuum robots enables the transmission of force from a distance, but it is understood that tension control problems can arise when a pulley is used to actuate two cables in a push-pull mode. This paper analyzes the relationship between angle of rotation, pressure, as well as variables of a single continuum unit in a quasi-static equilibrium. The primary objective of the quasi-static analysis was to output pressure and the analysis, given the tensions applied. Static equilibrium condition was established, and the bisection method was carried out for the angle of rotation. The function for the bisection method considered pressure-induced forces, friction forces, and weight. {\theta} was 17.14{\deg}, and p was 405.6 Pa when Tl and Ts were given the values of 1 N and 2 N, respectively. The results seemed to be consistent with the preliminary design specification, calling for further simulations and experiments

The Antiviral Effect of High-Molecular Weight Poly-Gamma-Glutamate against Newcastle Disease Virus on Murine Macrophage Cells

This study demonstrates the capacity of HM-γ-PGA treatment to significantly protect murine macrophage cells (RAW 264.7 cells) against NDV infection. Such protection can be explained by the induction of antiviral state of HM-γ-PGA in RAW 264.7 cells via TLR4-mediated IRF-3, IRF-7, IFN-β, and IFN-related gene induction as shown in time-dependent changes in mRNA expression confirmed by polymerase chain reaction (PCR). Moreover, the present research also showed that HM-γ-PGA can induce proinflammatory cytokine secretion in RAW 264.7 as measured by enzyme-linked immunosorbent assay (ELISA). Therefore, our findings suggest that HM-γ-PGA can be a potential antiviral substance that can inhibit NDV infection through its stimulation of antiviral state on RAW 264.7 cells. These results have been consistent with the previous studies showing that HM-γ-PGA can protect RAW 264.7 cells and mice against influenza infection. However, it should be noted that although murine macrophage cells are susceptible to NDV, they are not the natural host cells of the virus; thus further in vivo and in vitro studies involving chicken and chicken immune cells are needed to fully assess the efficacy and applicability of HM-γ-PGA in the poultry industry

Bis(benzyl­sulfan­yl)methane

In the title compound, C15H16S2, the structure of the dithioalkyl chain is a helix with an all-cis conformation. The dihedral angle between the mean planes of the terminal aromatic rings is 74.60 (4)°. In the crystal structure, weak C—H⋯π inter­actions contribute to the stabilization of the packing

Classification methods for the development of genomic signatures from high-dimensional data

Personalized medicine is defined by the use of genomic signatures of patients to assign effective therapies. We present Classification by Ensembles from Random Partitions (CERP) for class prediction and apply CERP to genomic data on leukemia patients and to genomic data with several clinical variables on breast cancer patients. CERP performs consistently well compared to the other classification algorithms. The predictive accuracy can be improved by adding some relevant clinical/histopathological measurements to the genomic data

Flutolanil [N-(3-isopropoxyphen­yl)-2-(trifluoro­meth­yl)benzamide]

The title compound, C17H16F3NO2, crystallizes with two independent mol­ecules in the asymmetric unit. The dihedral angles between the isopropoxyphenyl and trifluoro­methyl­phenyl rings are 85.78 (5) and 63.15 (6)° in the two mol­ecules. In the crystal structure, inter­molecular N—H⋯O and C—H⋯π inter­actions are observed

Cetaceans evolution:insights from the genome sequences of common minke whales

Background: Whales have captivated the human imagination for millennia. These incredible cetaceans are the only mammals that have adapted to life in the open oceans and have been a source of human food, fuel and tools around the globe. The transition from land to water has led to various aquatic specializations related to hairless skin and ability to regulate their body temperature in cold water. Results: We present four common minke whale (Balaenoptera acutorostrata) genomes with depth of ×13 ~ ×17 coverage and perform resequencing technology without a reference sequence. Our results indicated the time to the most recent common ancestors of common minke whales to be about 2.3574 (95% HPD, 1.1521 - 3.9212) million years ago. Further, we found that genes associated with epilation and tooth-development showed signatures of positive selection, supporting the morphological uniqueness of whales. Conclusions: This whole-genome sequencing offers a chance to better understand the evolutionary journey of one of the largest mammals on earth

Development of HRM Markers for Discrimination of Pyogo (Lentinula edodes) Cultivars Sanjo 701 and Chamaram

Pyogo (Shiitake, Lentinula edodes) is one of the most important edible mushrooms because of its outstanding nutritive and medicinal value. In the registration and protection procedure for newly developed mushroom cultivars, the application of molecular markers that can supplement the morphological characteristic-based distinction has been strongly requested. Sanjo 701 and Chamaram, newly developed at the Federation Forest Mushroom Research Center of Korea, have been characterized as innovative cultivars suitable for customer demands because of their high yields and cultivation rates. However, no technical tools can protect the rights to these important cultivars. In this study, using comparative genomic information from 23 commercially available pyogo cultivars, we identified single nucleotide polymorphisms (SNPs) that accurately differentiated Sanjo701 and Chamaram from the other cultivars. We also developed high-resolution melting analysis (HRM)-based SNP markers that discriminate among the tested 23 pyogo cultivars. The developed SNP markers can be utilized for rapid, accurate identification of pyogo cultivars with low genetic diversity and to prevent cultivar contamination caused by illegally distributed inocula. In addition, these markers can serve as a crucial scientific basis for securing the right to conserve new cultivars in international markets

Attribution of Tumor Lethality in the Absence of Cause-of-Death Information

A new statistical approach is developed for estimating the carcinogenic potential of drugs and other chemical substances used by humans. Improved statistical methods are developed for rodent tumorigenicity assays that have interval sacrifices but not cause-of-death data. For such experiments, this paper proposes a nonparametric maximum likelihood estimation method for estimating the distributions of time-to-onset-of and the time-to-death-from the tumor. The log-likelihood function is optimized using a constrained direct-search procedure. Using the maximum likelihood estimators, the number of fatal tumors in an experiment can be imputed. A Monte Carlo Simulation study is conducted for illustration. The accuracy of cause of death attributed by the proposed method tends to increase as the competing risks survival rate increases. By applying the proposed procedure to a real data set, the effect of calorie restriction is investigated. In this study, we found calorie restriction delays the t..

Detection and Classification of Demagnetization and Interturn Short Faults of IPMSMs

We present a method that detects and classifies demagnetization and interturn short faults (ISFs), which degrade the performance of permanent-magnet synchronous machines (PMSMs). Demagnetization and ISF are analyzed using models; we focused on changes of magnitude and angle of currents in the synchronous frame. Those two faults increase the magnitude of the input current compared with normal machines at the same load torque. However, our analysis suggests that demagnetization causes increase in the current angle beta, whereas ISF causes decrease in beta. We exploit this difference in response to classify the fault if one is detected in the PMSM. Experimental studies on interior-type PMSMs verify that demagnetization and ISF can be detected and classified in various operation conditions.113sciescopu