967 research outputs found
Social bonds and genetic ties: Kinship association and affiliation in a community of bonobos (Pan paniscus)
Studies of captive populations of bonobos suggest that females are more gregarious than males. This seems to contradict assumed sex-differences in kinship deriving from a speciestypical dispersal pattern of female exogamy and male philopatry. Here we present data on spatial associations and affiliative relations among members of one wild community (Eyengo) for which genetic relationships were identified by analysing mitochondrial and nuclear DNA. Our data from Lomako confirm the existence of spatial associations among resident females. In addition, they reveal strong social bonds between males and females. While most female-female associations did not last longer than one field season, long-term associations occurred predominantly between mixed-sex dyads and involved both close kin and unrelated individuals. Differences in social grooming appeared to be related to patterns of spatial association rather than to kinship. It is suggested that under natural conditions social organisation of bonobos is characterised by strong inter-sexual bonds. Males may benefit from bonding with females by increased reproductive success via rank acquisition. For females benefits may derive from inclusive fitness and reduced food competition. Preliminary evidence suggests that females also may benefit from protection by resident males against male intruders
Teachers as mediators: an exploration of situated English teaching
Within the context of lower secondary English teaching in South West England, this study identifies in broad terms the competing goals between which English teachers mediate and the explicit and hidden tensions that result. To understand the interactions of competing goals, teachers’ goal-oriented behaviours are referenced to a set of idealised ‘role types’ based on the dimensions of goals, norms, discourses and practices. It is asserted that competing goals, significant to particular educational circumstances, emanate from various sometimes contradictory local, national and perhaps broader social and cultural influences on practice. Yet the teachers observed moved smoothly between goal-oriented behaviours in a continuous and comfortable style, easily and without reflecting any tensions between them. Thus, this article elaborates an account of situated English teaching
Varieties of teacher expertise in teaching Danish language and literature in lower secondary schools
Ultrastructural analysis of chromatin in meiosis I plus II of rye (Secale cereale L.)
Scanning electron microscopy (SEM) proves to be an appropriate technique for imaging chromatin organization in meiosis I and II of rye (Secale cereale) down to a resolution of a few nanometers. It could be shown for the first time that organization of basic structural elements (coiled and parallel fibers, chromomeres) changes dramatically during the progression to metaphase I and II. Controlled loosening with proteinase K (after fixation with glutaraldehyde) provides an enhanced insight into chromosome architecture even of highly condensed stages of meiosis. By selective staining with platinum blue, DNA content and distribution can be visualized within compact chromosomes as well as in a complex arrangement of fibers. Chromatin interconnecting threads, which are typically observed in prophase I between homologous and non-homologous chromosomes, stain clearly for DNA. In zygotene transversion of chromatid strands to their homologous counterparts becomes evident. In pachytene segments of synapsed and non-synapsed homologs alternate. At synapsed regions pairing is so intimate that homologous chromosomes form one filament of structural entity. Chiasmata are characterized by chromatid strands which traverse from one homolog to its counterpart. Bivalents are characteristically fused at their telomeric regions. In metaphase I and II there is no structural evidence for primary and secondary constrictions. Copyright (C) 2003 S. Karger AG, Basel
MACC1 driven alterations in cellular biomechanics facilitate cell motility in glioblastoma
BACKGROUND: Metastasis-associated in colon cancer 1 (MACC1) is an established marker for metastasis and tumor cell migration in a multitude of tumor entities, including glioblastoma (GBM). Nevertheless, the mechanism underlying the increased migratory capacity in GBM is not comprehensively explored. METHODS: We performed live cell and atomic force microscopy measurements to assess cell migration and mechanical properties of MACC1 overexpressing GBM cells. We quantified MACC1 dependent dynamics of 3D aggregate formation. For mechanistic studies we measured the expression of key adhesion molecules using qRT-PCR, and MACC1 dependent changes in short term adhesion to fibronectin and laminin. We then determined changes in sub-cellular distribution of integrins and actin in dependence of MACC1, but also in microtubule and intermediate filament organization. RESULTS: MACC1 increased the migratory speed and elastic modulus of GBM cells, but decreased cell-cell adhesion and inhibited the formation of 3D aggregates. These effects were not associated with altered mRNA expression of several key adhesion molecules or altered short-term affinity to laminin and fibronectin. MACC1 did neither change the organization of the microtubule nor intermediate filament cytoskeleton, but resulted in increased amounts of protrusive actin on laminin. CONCLUSION: MACC1 overexpression increases elastic modulus and migration and reduces adhesion of GBM cells thereby impeding 3D aggregate formation. The underlying molecular mechanism is independent on the organization of microtubules, intermediate filaments and several key adhesion molecules, but depends on adhesion to laminin. Thus, targeting re-organization of the cytoskeleton and cell motility via MACC1 may offer a treatment option to impede GBM spreading
MACC1-induced collective migration is promoted by proliferation rather than single cell biomechanics
Metastasis-associated in colon cancer 1 (MACC1) is a marker for metastasis, tumor cell migration, and increased proliferation in colorectal cancer (CRC). Tumors with high MACC1 expression show a worse prognosis and higher invasion into neighboring structures. Yet, many facets of the pro-migratory effects are not fully understood. Atomic force microscopy and single cell live imaging were used to quantify biomechanical and migratory properties in low- and high-MACC1-expressing CRC cells. Furthermore, collective migration and expansion of small, cohesive cell colonies were analyzed using live cell imaging and particle image velocimetry. Lastly, the impact of proliferation on collective migration was determined by inhibition of proliferation using mitomycin. MACC1 did not affect elasticity, cortex tension, and single cell migration of CRC cells but promoted collective migration and colony expansion in vitro. Measurements of the local velocities in the dense cell layers revealed proliferation events as regions of high local speeds. Inhibition of proliferation via mitomycin abrogated the MACC1-associated effects on the collective migration speeds. A simple simulation revealed that the expansion of cell clusters without proliferation appeared to be determined mostly by single cell properties. MACC1 overexpression does not influence single cell biomechanics and migration but only collective migration in a proliferation-dependent manner. Thus, targeting proliferation in high-MACC1-expressing tumors may offer additional effects on cell migration
Non-invasive genetic approaches for estimation of ungulate population size: a study on roe deer (Capreolus capreolus) based on faeces
Estudios genéticos no invasivos, para la estimación del tamaño de una población de ungulados: estudio sobre el corzo (Capreolus capreolus) basado en sus heces
La estimación de los tamaños de población es particularmente difícil en las especies de animales que viven en hábitats de vegetación densa, en la que se pueden mimetizar. Este es el caso del corzo, al igual que el de muchos otros ungulados. Nuestro objetivo fue desarrollar una aproximación genética no invasiva de captura–marcado–recaptura basada en las heces de corzo recogidas a lo largo de transectos. En un estudio piloto, recogimos 1.790 heces de corzo durante cinco días de muestreo en un área de estudio boscosa en el sudoeste de Alemania. Extrajimos el ADN de 410 de dichas muestras y llevamos a cabo un análisis de microsatélites utilizando siete marcadores de dinucleótidos. Los análisis tuvieron como resultado 328 genotipos consenso, que se asignaron a 174 individuos. La población estimada usando el enfoque bayesiano fue de 94 (82–111) machos y 136 (121–156) hembras. Nuestro estudio demuestra que los métodos genéticos no invasivos constituyen una herramienta de gestión muy valiosa para el corzo.Estudios genéticos no invasivos, para la estimación del tamaño de una población de ungulados: estudio sobre el corzo (Capreolus capreolus) basado en sus heces
La estimación de los tamaños de población es particularmente difícil en las especies de animales que viven en hábitats de vegetación densa, en la que se pueden mimetizar. Este es el caso del corzo, al igual que el de muchos otros ungulados. Nuestro objetivo fue desarrollar una aproximación genética no invasiva de captura–marcado–recaptura basada en las heces de corzo recogidas a lo largo de transectos. En un estudio piloto, recogimos 1.790 heces de corzo durante cinco días de muestreo en un área de estudio boscosa en el sudoeste de Alemania. Extrajimos el ADN de 410 de dichas muestras y llevamos a cabo un análisis de microsatélites utilizando siete marcadores de dinucleótidos. Los análisis tuvieron como resultado 328 genotipos consenso, que se asignaron a 174 individuos. La población estimada usando el enfoque bayesiano fue de 94 (82–111) machos y 136 (121–156) hembras. Nuestro estudio demuestra que los métodos genéticos no invasivos constituyen una herramienta de gestión muy valiosa para el corzo.Estimating population size is particularly difficult for animal species living in concealing habitats with dense vegetation. This is the case for roe deer as for many other ungulates. Our objective was to develop a non–invasive genetic capture–mark–recapture approach based on roe deer faeces collected along transects. In a pilot study, we collected 1,790 roe deer faeces during five sampling days in a forested study area in south western Germany. We extracted DNA from 410 of these samples and carried out microsatellite analysis using seven dinucleotide markers. The analyses resulted in 328 useable consensus genotypes which were assigned to 174 individuals. The population size estimated using a Bayesian approach was 94 (82–111) male and 136 (121–156) female roe deer. Our study shows that non–invasive genetic methods are a valuable management tool for roe deer
Small catchment runoff sensitivity to station density and spatial interpolation: Hydrological modeling of heavy rainfall using a dense rain Gauge network
Precipitation is the most important input to hydrological models, and its spatial variability can strongly influence modeled runoff. The highly dense station network WegenerNet (0.5 stations per km2) in southeastern Austria offers the opportunity to study the sensitivity of modeled runoff to precipitation input. We performed a large set of runoff simulations (WaSiM model) using 16 subnetworks with varying station densities and two interpolation schemes (inverse distance weighting, Thiessen polygons). Six representative heavy precipitation events were analyzed, placing a focus on small subcatchments (10–30 km2) and different event durations. We found that the modeling performance generally improved when the station density was increased up to a certain resolution: a mean nearest neighbor distance of around 6 km for long-duration events and about 2.5 km for short-duration events. However, this is not always true for small subcatchments. The sufficient station density is clearly dependent on the catchment area, event type, and station distribution. When the network is very dense (mean distance < 1.7 km), any reasonable interpolation choice is suitable. Overall, the station density is much more important than the interpolation scheme. Our findings highlight the need to study extreme precipitation characteristics in combination with runoff modeling to decompose precipitation uncertainties more comprehensively
CTGF (IGFBP-rP2) is specifically expressed in malignant lymphoblasts of patients with acute lymphoblastic leukaemia (ALL)
Connective tissue growth factor (CTGF) is a major chemotactic and mitogenic factor for connective tissue cells. The amino acid sequence shares an overall 28–38% identity to IGFBPs and contains critical conserved sequences in the amino terminus. It has been demonstrated that human CTGF specifically binds IGFs with low affinity and is considered to be a member of the IGFBP superfamily (IGFBP-rP2). In the present study, the expression of CTGF (IGFBP-rP2) in human leukaemic lymphoblasts from children with acute lymphoblastic leukaemia (ALL) was investigated. RNA samples from tumour clones enriched by ficoll separation of bone marrow or peripheral blood mononuclear cells (MNC) from 107 patients with childhood ALL at diagnosis and 57 adult patients with chronic myeloid leukaemia (CML) were studied by RT-PCR. In addition MNC samples from children with IDDM and cord blood samples from healthy newborns were investigated as control groups. Sixty-one percent of the patients with ALL (65 of 107) were positive for CTGF (IGFBP-rP2) expression. In the control groups, no expression of CTGF (IGFBP-rP2) in peripheral MNC was detected, and in the group of adult CML patients only 3.5% (2 of 57) were positive for this gene. The role of CTGF (IGFBP-rP2) in lymphoblastic leukaemogenesis requires further evaluation, as does its potential utility as a tumour marker. © 2000 Cancer Research Campaig
Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors.
Plant-unique membrane receptor kinases with leucine-rich repeat ectodomains (LRR-RKs) can sense small molecule, peptide, and protein ligands. Many LRR-RKs require SERK-family coreceptor kinases for high-affinity ligand binding and receptor activation. How one coreceptor can contribute to the specific binding of distinct ligands and activation of different LRR-RKs is poorly understood. Here we quantitatively analyze the contribution of SERK3 to ligand binding and activation of the brassinosteroid receptor BRI1 and the peptide hormone receptor HAESA. We show that while the isolated receptors sense their respective ligands with drastically different binding affinities, the SERK3 ectodomain binds the ligand-associated receptors with very similar binding kinetics. We identify residues in the SERK3 N-terminal capping domain, which allow for selective steroid and peptide hormone recognition. In contrast, residues in the SERK3 LRR core form a second, constitutive receptor-coreceptor interface. Genetic analyses of protein chimera between BRI1 and SERK3 define that signaling-competent complexes are formed by receptor-coreceptor heteromerization in planta. A functional BRI1-HAESA chimera suggests that the receptor activation mechanism is conserved among different LRR-RKs, and that their signaling specificity is encoded in the kinase domain of the receptor. Our work pinpoints the relative contributions of receptor, ligand, and coreceptor to the formation and activation of SERK-dependent LRR-RK signaling complexes regulating plant growth and development
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