Lateglacial and Holocene vegetation history in the Insubrian Southern Alps—New indications from a small-scale site

Fundamental uncertainties exist in the study region about the former lowland vegetation at local scales. All existing palaeoecological results are derived from sediments of medium- to large-sized sites (8-5000ha), which are thought to record mainly regional vegetation in their pollen content. Therefore the very small mire at Balladrum (0.05ha) was analysed for pollen, plant-macrofossils, and charcoal and the results compared with those of previous studies in the same region. Common regional signals were detected, but also new insights for the tree species Pinus cembra (L.), Abies alba (Mill.) and Castanea sativa (Mill.). Our palaeobotanical data reveal the local dominance of the timberline species P. cembra during the Lateglacial (16500-14250 cal b.p.) at low-altitudes. For A. alba an early presence in the area is suggested by pollen data, corroborating previous high-altitudinal studies indicating the presence of glacial refugia in the region. Occasional findings of C. sativa pollen throughout the Holocene may indicate the local but very rare presence of this species in the Insubrian Southern Alps, in contrast to the conventional opinion that C. sativa was introduced during the Roman Period. Altogether the results confirm the need of multiproxy palaeobotanical records from basins of variable size to assess the past composition of vegetation more accuratel

Identification of 2,4-Dinitro-Biphenyl-Based Compounds as MAPEG Inhibitors

We identified 2,4-dinitro-biphenyl-based compounds as new inhibitors of leukotriene C-4 synthase (LTC4S) and 5-lipoxygenase-activating protein (FLAP), both members of the "Membrane Associated Proteins in Eicosanoid and Glutathione metabolism" (MAPEG) family involved in the biosynthesis of pro-inflammatory eicosanoids. By molecular docking we evaluated the putative binding against the targets of interest, and by applying cell-free and cell-based assays we assessed the inhibition of LTC4S and FLAP by the small molecules at low micromolar concentrations. The present results integrate the previously observed inhibitory profile of the tested compounds against another MAPEG member, i. e., microsomal prostaglandin E-2 synthase (mPGES)-1, suggesting that the 2,4-dinitro-biphenyl scaffold is a suitable molecular platform for a multitargeting approach to modulate pro-inflammatory mediators in inflammation and cancer treatment