736 research outputs found
Alternative model for the administration and analysis of research-based assessments
Research-based assessments represent a valuable tool for both instructors and
researchers interested in improving undergraduate physics education. However,
the historical model for disseminating and propagating conceptual and
attitudinal assessments developed by the physics education research (PER)
community has not resulted in widespread adoption of these assessments within
the broader community of physics instructors. Within this historical model,
assessment developers create high quality, validated assessments, make them
available for a wide range of instructors to use, and provide minimal (if any)
support to assist with administration or analysis of the results. Here, we
present and discuss an alternative model for assessment dissemination, which is
characterized by centralized data collection and analysis. This model provides
a greater degree of support for both researchers and instructors in order to
more explicitly support adoption of research-based assessments. Specifically,
we describe our experiences developing a centralized, automated system for an
attitudinal assessment we previously created to examine students'
epistemologies and expectations about experimental physics. This system
provides a proof-of-concept that we use to discuss the advantages associated
with centralized administration and data collection for research-based
assessments in PER. We also discuss the challenges that we encountered while
developing, maintaining, and automating this system. Ultimately, we argue that
centralized administration and data collection for standardized assessments is
a viable and potentially advantageous alternative to the default model
characterized by decentralized administration and analysis. Moreover, with the
help of online administration and automation, this model can support the
long-term sustainability of centralized assessment systems.Comment: 7 pages, 1 figure, accepted in Phys. Rev. PE
Using GIS to Teach Placed-Based Mathematics in Rural Classrooms
The purpose of this article is to promote the use of GIS and place-based education (PBE) in rural mathematics classrooms. The pedagogy of place is disappearing from rural communities because of declining enrollments, lack of support, and federal mandates to focus more on basic academic skills. However, PBE does not stand in opposition to standards-based instruction and academic achievement; rather, it enhances instructional strategies for getting at these aims. We present examples of place that can be used to engage rural students in meaningful mathematics activities to improve their content knowledge and problem-solving ability. Barriers exist to full implementation of this work. Yet, we offer a vision of what is possible through the use of technological tools like GIS for teachers who teach in rural communities. Additional studies regarding the effect of using GIS are needed to bring the vision of situated place-based education closer to fruition
Cathepsin B contributes to Na + hyperabsorption in cystic fibrosis airway epithelial cultures: Cathepsin B and ENaC
In cystic fibrosis (CF) lung disease, the absence of functional CF transmembrane conductance regulator results in Cl−/HCO3− hyposecretion and triggers Na+ hyperabsorption through the epithelial Na+ channel (ENaC), which contribute to reduced airway surface liquid (ASL) pH and volume. Prostasin, a membrane-anchored serine protease with trypsin-like substrate specificity has previously been shown to activate ENaC in CF airways. However, prostasin is typically inactive below pH 7.0, suggesting that it may be less relevant in acidic CF airways. Cathepsin B (CTSB) is present in both normal and CF epithelia and is secreted into ASL, but little is known about its function in the airways. We hypothesized that the acidic ASL seen in CF airways may stimulate CTSB to activate ENaC, contributing to Na+ hyperabsorption and depletion of CF ASL volume. In Xenopus laevis oocytes, CTSB triggered α- and γENaC cleavage and induced an increase in ENaC activity. In bronchial epithelia from both normal and CF donor lungs, CTSB localized to the apical membrane. In normal and CF human bronchial epithelial cultures, CTSB was detected at the apical plasma membrane and in the ASL. CTSB activity was significantly elevated in acidic ASL, which correlated with increased abundance of ENaC in the plasma membrane and a reduction in ASL volume. This acid/CTSB-dependent activation of ENaC was ameliorated with the cell impermeable, CTSB-selective inhibitor CA074, suggesting that CTSB inhibition may have therapeutic relevance. Taken together, our data suggest that CTSB is a pathophysiologically relevant protease that activates ENaC in CF airways
Ancient channels of the Susquehanna River beneath Chesapeake Bay and the Delmarva Peninsula
Three generations of the ancestral Susquehanna River system have been mapped beneath Chesapeake Bay and the southern Delmarva Peninsula. Closely spaced seismic reflection profiles in the bay and boreholes in the bay and on the southern Delmarva Peninsula allow detailed reconstruction of each paleochannel system. The channel systems were formed during glacial low sea-level stands, and each contains a channel-fill sequence that records the subsequent transgression. The trunk channels of each system are 2 to 4 km wide and are incised 30 to SO m into underlying strata; they have irregular longitudinal profiles and very low gradients within the Chesapeake Bay area
WholePathwayScope: a comprehensive pathway-based analysis tool for high-throughput data
BACKGROUND: Analysis of High Throughput (HTP) Data such as microarray and proteomics data has provided a powerful methodology to study patterns of gene regulation at genome scale. A major unresolved problem in the post-genomic era is to assemble the large amounts of data generated into a meaningful biological context. We have developed a comprehensive software tool, WholePathwayScope (WPS), for deriving biological insights from analysis of HTP data. RESULT: WPS extracts gene lists with shared biological themes through color cue templates. WPS statistically evaluates global functional category enrichment of gene lists and pathway-level pattern enrichment of data. WPS incorporates well-known biological pathways from KEGG (Kyoto Encyclopedia of Genes and Genomes) and Biocarta, GO (Gene Ontology) terms as well as user-defined pathways or relevant gene clusters or groups, and explores gene-term relationships within the derived gene-term association networks (GTANs). WPS simultaneously compares multiple datasets within biological contexts either as pathways or as association networks. WPS also integrates Genetic Association Database and Partial MedGene Database for disease-association information. We have used this program to analyze and compare microarray and proteomics datasets derived from a variety of biological systems. Application examples demonstrated the capacity of WPS to significantly facilitate the analysis of HTP data for integrative discovery. CONCLUSION: This tool represents a pathway-based platform for discovery integration to maximize analysis power. The tool is freely available at
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