Development of a high-density CHO-C system enables rapid protein production in 10 days

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Robust Design of SAW Gas Sensors by Taguchi Dynamic Method

This paper adopts Taguchi’s signal-to-noise ratio analysis to optimize the dynamic characteristics of a SAW gas sensor system whose output response is linearly related to the input signal. The goal of the present dynamic characteristics study is to increase the sensitivity of the measurement system while simultaneously reducing its variability. A time- and cost-efficient finite element analysis method is utilized to investigate the effects of the deposited mass upon the resonant frequency output of the SAW biosensor. The results show that the proposed methodology not only reduces the design cost but also promotes the performance of the sensors

A New SFF Process for Functional Ceramic Components

SRI International in collaboration with Saint-Gobain/Norton Industrial Ceramics Corporation and Allison Engine Company is developing a new SFF process, Direct Photo Shaping, for the fabrication of functional ceramic components. The process is based on the layerby-layer photocuring of ceramic slurries containing monomers curable by visible light. Each layer is photoimaged by a liquid crystal display (LCD) or a digital light processing (DLP) projection system. After binder removal and sintering, the properties of silicon nitride tiles prepared according to the Dire~t Photo Shaping process were found to be comparable to properties of tiles formed by conventional methods.Mechanical Engineerin

Reduction of Artefacts in JPEG-XR Compressed Images

The JPEG-XR encoding process utilizes two types of transform operations: Photo Overlap Transform (POT) and Photo Core Transform (PCT). Using the Device Porting Kit (DPK) provided by Microsoft, we performed encoding and decoding processes on JPEG XR images. It was discovered that when the quantization parameter is >1-lossy compression conditions, the resulting image displays chequerboard block artefacts, border artefacts and corner artefacts. These artefacts are due to the nonlinearity of transforms used by JPEG-XR. Typically, it is not so visible; however, it can cause problems while copying and scanning applications, as it shows nonlinear transforms when the source and the target of the image have different configurations. Hence, it is important for document image processing pipelines to take such artefacts into account. Additionally, these artefacts are most problematic for high-quality settings and appear more visible at high compression ratios. In this paper, we analyse the cause of the above artefacts. It was found that the main problem lies in the step of POT and quantization. To solve this problem, the use of a “uniform matrix” is proposed. After POT (encoding) and before inverse POT (decoding), an extra step is added to multiply this uniform matrix. Results suggest that it is an easy and effective way to decrease chequerboard, border and corner artefacts, thereby improving the image quality of lossy encoding JPEG XR than the original DPK program with no increased calculation complexity or file size

Bamboo vinegar decreases inflammatory mediator expression and NLRP3 inflammasome activation by inhibiting reactive oxygen species generation and protein kinase C-α/δ activation.

Bamboo vinegar (BV), a natural liquid derived from the condensation produced during bamboo charcoal production, has been used in agriculture and as a food additive, but its application to immune modulation has not been reported. Here, we demonstrated that BV has anti-inflammatory activities both in vitro and in vivo. BV reduced inducible nitric oxide synthase expression and nitric oxide levels in, and interleukin-6 secretion by, lipopolysaccharide-activated macrophages without affecting tumor necrosis factor-α secretion and cyclooxygenase-2 expression. The mechanism for the anti-inflammatory effect of BV involved decreased reactive oxygen species production and protein kinase C-α/δ activation. Furthermore, creosol (2-methoxy-4-methylphenol) was indentified as the major anti-inflammatory compound in BV. Impaired cytokine expression and NLR family, pyrin domain-containing 3 (NLRP3) inflammasome activation was seen in mice treated with creosol. These findings provide insights into how BV regulates inflammation and suggest that it may be a new source for the development of anti-inflammatory agents or a healthy supplement for preventing and ameliorating inflammation- and NLRP3 inflammasome-related diseases, including metabolic syndrome

Citral is renoprotective for focal segmental glomerulosclerosis by inhibiting oxidative stress and apoptosis and activating Nrf2 pathway in mice.

The pathogenesis of focal segmental glomerulosclerosis (FSGS) is considered to be associated with oxidative stress, mononuclear leukocyte recruitment and infiltration, and matrix production and/or matrix degradation, although the exact etiology and pathogenic pathways remain to be determined. Establishment of a pathogenesis-based therapeutic strategy for the disease is clinically warranted. Citral (3,7-dimethyl-2,6-octadienal), a major active compound in Litseacubeba, a traditional Chinese herbal medicine, can inhibit oxidant activity, macrophage and NF-κB activation. In the present study, first, we used a mouse model of FSGS with the features of glomerular epithelial hyperplasia lesions (EPHLs), a key histopathology index of progression of FSGS, peri-glomerular inflammation, and progressive glomerular hyalinosis/sclerosis. When treated with citral for 28 consecutive days at a daily dose of 200 mg/kg of body weight by gavage, the FSGS mice showed greatly reduced EPHLs, glomerular hyalinosis/sclerosis and peri-glomerular mononuclear leukocyte infiltration, suggesting that citral may be renoprotective for FSGS and act by inhibiting oxidative stress and apoptosis and early activating the Nrf2 pathway. Meanwhile, a macrophage model involved in anti-oxidative and anti-inflammatory activities was employed and confirmed the beneficial effects of citral on the FSGS model