NOx and extremely low temperatures of charging

The basis of basic research consists in the influence monitoring of unconventional (extremely low temperatures) charging intercooling on the selected ecological and economical parameters of a turbocharged internal combustion engine in the specific operational conditions (periodic starts and stops). The paper deals with the influence of the mentioned temperatures from the point of view of the harmful gaseous emissions production withfocus on NOx. The theoretical analysis, consequential mathematical modelling (in Fluent) of the utilizing of the extremely low temperatures of charging after the intercooler in the range from 30°C to near above zero temperatures of the turbocharged engine should define the changes trends in its power parameters and exhaust gas emissions production mostly NOx. At the same time it should consider the influence of the extremely low temperatures of charging on the engine inner corrosion at the periodic engine starts and stops. The mathematical model should allow alternatively solving possible risks resulting from the proposal newness and originality. From the proposal it is expected to obtain more effective energy utilization, the decrease of the environment ecological load compared with the standard solution and the possibility of creation of the permanent sustainable energetic system. The possibilities of the design solution for the system of extremely low temperatures of air fuel mixture are also presented in the paper

Evaluation of alpha-methylacyl-CoA racemase, metallothionein and prostate specific antigen as prostate cancer prognostic markers. Neoplasma 59: 191

Current diagnostic techniques are inefficient in distinguishing latent and low-risk forms of prostate cancer from high-risk forms. The present study is focused on determination of putative tumor markers of aggressive high-grade forms of prostate cancer. Potential markers were determined in blood sera of 133 patients (82 cases and 51 controls) and in cell lines (Gleason score 9-derived 22Rv1 and normal tissue derived PNT1A) on mRNA and protein levels. Alpha-methylacyl-CoA racemase (AMACR), metallothionein classes 1A and 2A (MT1A and MT2A) were determined and compared to prostate specific antigen (PSA) levels. On mRNA level, significantly increased expression of MT2A (2.4-fold), PSA (2.6-fold) and AMACR (8.4-fold) and insignificantly (1.9-fold) elevated MT1A in 22Rv1 compared to non-tumor PNT1A were determined. On protein level, significant enhancement of free PSA and total PSA in tumor cell line was evident. AMACR protein was 1.5-fold elevated in tumor line (below the level of significance). Contrary to mRNA, significantly (p = 0.01) reduced level of MT protein in tumor lines was determined. In the case of serum level, significantly enhanced MT level (4.5-fold) in patients' sera was found. No significant changes were observed in the case of AMACR. These findings indicate possible alternative role of MT to PSA prostate cancer marker. In addition, level of AMACR is distinctly higher in the Gleason score 9 in serum of patients and MT shows a descending trend in relation to Gleason score