6 research outputs found
Electronically Type-Sorted Carbon Nanotube-Based Electrochemical Biosensors with Glucose Oxidase and Dehydrogenase
An electrochemical enzyme biosensor
with electronically type-sorted
(metallic and semiconducting) single-walled carbon nanotubes (SWNTs)
for use in aqueous media is presented. This research investigates
how the electronic types of SWNTs influence the amperometric response
of enzyme biosensors. To conduct a clear evaluation, a simple layer-by-layer
process based on a plasma-polymerized nano thin film (PPF) was adopted
because a PPF is an inactive matrix that can form a well-defined nanostructure
composed of SWNTs and enzyme. For a biosensor with the glucose oxidase
(GOx) enzyme in the presence of oxygen, the response of a metallic
SWNT-GOx electrode was 2 times larger than that of a semiconducting
SWNT-GOx electrode. In contrast, in the absence of oxygen, the response
of the semiconducting SWNT-GOx electrode was retained, whereas that
of the metallic SWNT-GOx electrode was significantly reduced. This
indicates that direct electron transfer occurred with the semiconducting
SWNT-GOx electrode, whereas the metallic SWNT-GOx electrode was dominated
by a hydrogen peroxide pathway caused by an enzymatic reaction. For
a biosensor with the glucose dehydrogenase (GDH; oxygen-independent
catalysis) enzyme, the response of the semiconducting SWNT-GDH electrode
was 4 times larger than that of the metallic SWNT-GDH electrode. Electrochemical
impedance spectroscopy was used to show that the semiconducting SWNT
network has less resistance for electron transfer than the metallic
SWNT network. Therefore, it was concluded that semiconducting SWNTs
are more suitable than metallic SWNTs for electrochemical enzyme biosensors
in terms of direct electron transfer as a detection mechanism. This
study makes a valuable contribution toward the development of electrochemical
biosensors that employ sorted SWNTs and various enzymes
Characterization of Nitrogen-Rich Coating Films with Atmospheric-Pressure Plasma Generated by Re-Entrant Microwave Cavity
The deposition and characterization
of nitrogen-rich coating films
using atmospheric-pressure plasma generated with a re-entrant cylindrical
microwave cavity is presented. This system enables simple matching,
stable plasma, and free space under the orifice of plasma steam. Allylamine
and acetonitrile are employed as monomers, whereas argon is used as
the carrier gas. The effective area of the hydrophilic coating film
is 55 mm in diameter and the deposition rate is 10 nm min<sup>–1</sup>. X-ray photoelectron spectroscopy measurements show that the surfaces
of these films contain a high concentration of nitrogen atoms and
primary amine groups. Matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry shows that the coating films have
a large molecular weight (>200 kDa). The surface morphology is
very
flat (ca. 1 nm). The experimental results indicate that a highly cross-linked
three-dimensional polymer matrix is formed and atmospheric-pressure
plasma deposition is successfully achieved
Electrochemical Study of Dopamine at Electrode Fabricated by Cellulose-Assisted Aqueous Dispersion of Long-Length Carbon Nanotube
A long-length
(hundred micrometers) carbon nanotube is successfully
dispersed in aqueous solution with surfactant cellulose while maintaining
its length. An electrochemical study of the synthetic pathway of dopamine
(DA), dopamine-<i>o</i>-quinone (DAQ), leucodopaminechrome
(LDAC), and dopaminechrome (DAC) at the electrode fabricated by the
long-length carbon nanotube dispersed solution is presented. The sequence
DA ⇌ DAQ ⇀ LDAC ⇌ DAC for the reaction is electron
transfer-chemical reaction-electron transfer (ECE)-type, which is
a chemical reaction (DAQ ⇀ LDAC, C) interposed between two
electron transfer reactions (DA ⇌ DAQ and LDAC ⇌ DAC,
E). The salient electrochemical signals due to both DA ⇌ DAQ
and LDAC ⇌ DAC can be obtained at the long-length carbon nanotube
electrode, unlike other carbon electrodes such as carbon paste, graphene,
fullerene, nanofiber, and graphite. The overall reaction is dominated
by step DAQ ⇀ LDAC and is sensitive to pH. With cyclic voltammetry
in acidic media, the peak current due to LDAC ⇀ DAC disappeared
at a higher scan rate because the reaction rate for DAQ ⇀ LDAC
was so slow that DAQ was completely consumed in the electron transfer
of DAQ ⇀ DA before the chemical reaction of DAQ ⇀ LDAC
could go forward. In alkaline media, the peak height due to DAC ⇀
LDAC became as high as that due to DA ⇀ DAQ because the DAQ
⇀ LDAC rate became fast enough that a sufficient amount of
LDAC was generated for the subsequent reaction of LDAC ⇀ DAC.
Concomitantly, the reaction DAQ + LDAC ⇌ DA + DAC was generated.
Quantitative and selective detection of dopamine based on the signal
due to LDAC ⇀ DAC is possible just as in the conventional strategy
of direct oxidation of dopamine (DA ⇀ DAQ)
Mediatorless Direct Electron Transfer between Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase and Single-Walled Carbon Nanotubes
The
flavoenzymes flavin adenine dinucleotide-dependent glucose
dehydrogenase (FAD-GDH) and oxidase (FAD-GOx) do not undergo direct
electron transfer (DET) at conventional electrodes, because the flavin
adenine dinucleotide (FAD) cofactor is buried deeply (∼1.4
nm) below the protein surface. We present a mediator-less DET between
oxygen-insensitive FAD-GDH and single-walled carbon nanotubes (SWCNTs).
A glucose-concentration-dependent current (GCDC) is observed at the
electrode with the combination of glycosylated FAD-GDH and debundled
SWCNTs; the GCDC, because of an increase in the polarized potential
during potential sweep voltammetry, increases steeply (+0.1 V of onset,
1.2 mA cm<sup>–2</sup> at +0.6 V 48 mM glucose) without the
appearance of the FAD redox peak at −0.45 V. In the control
experiment, the GCDC is not observed at the counterpart with either
bundled SWCNTs or debundled multiwalled carbon nanotubes (MWCNTs).
In the control experiment, the GCDC is observed at an analogous electrode
based on oxygen-sensitive FAD-GOx with all CNT types (bundled SWCNTs,
debundled SWCNTs, and debundled MWCNTs) in the presence of oxygen
because oxygen acts as a natural and mobile mediator. Therefore, observation
of the GCDC at the electrode with oxygen-insensitive FAD-GDH and debundled
SWCNTs provides evidence of mediator-less DET, even though oxygen
is present. Details of the DET are discussed with respect to the recently
reported crystallographic model of FAD-GDH. The three-dimensional
globular FAD-GDH molecule is 4.5 nm × 5.6 nm × 7.8 nm, which
is larger than the 1.2 nm diameter of an individual SWCNT and smaller
than the 10 nm diameter of an individual MWCNT and the 1 μm
size of a SWCNT bundle. Only individual SWCNTs can be plugged into
the groove of FAD-GDH, which is close to and within 1.0 nm of FAD,
while maintaining their catalytic activity. Images obtained using
transmission electron and atomic force microscopies support the stated
configuration of FAD-GDH molecules and debundled SWCNTs. We demonstrate
that DET can be explained by quantum tunneling theory. Electrochemical
experiments with various FAD-GDHs suggest that (i) DET with debundling
SWCNT can be applied to any type of FAD-GDH, (ii) the electrode with
various types of FAD-GDH implements superior functions (compared to
an analogous electrode with FAD-GOx and nicotineamide adenine dinucleotide-GDH),
and (iii) glycan chains present on FAD-GDH prevent denaturation when
the SWCNT is close to FAD
Thermophilic <i>Talaromyces emersonii</i> Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase Bioanode for Biosensor and Biofuel Cell Applications
Flavin adenine dinucleotide (FAD)-dependent
glucose dehydrogenase
(GDH) was identified and cloned from thermophilic filamentous fungi Talaromyces emersonii using the homology cloning
method. A direct electron transfer bioanode composed of T. emersonii FAD-GDH and a single-walled carbon nanotube
was produced. Enzymes from thermophilic microorganisms generally have
low activity at ambient temperature; however, the T.
emersonii FAD-GDH bioanode exhibits a large anodic
current due to the enzymatic reaction (1 mA cm<sup>–2</sup>) at ambient temperature. Furthermore, the T. emersonii FAD-GDH bioanode worked at 70 °C for 12 h. This is the first
report of a bioanode with a glucose-catalyzing enzyme from a thermophilic
microorganism that has potential for biosensor and biofuel cell applications.
In addition, we demonstrate how the glycoforms of T.
emersonii FAD-GDHs expressed by various hosts influence
the electrochemical properties of the bioanode
Erratum: Yoshimi, Y., et al. Size of Heparin-Imprinted Nanoparticles Reflects the Matched Interactions with the Target Molecule. Sensors 2019, 19, 2415
The authors wish to make the following erratum to this paper [1]: The affliation 5 of co-author Ewa Moczko should be corrected into: “Departamento de Química Ambiental, Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Concepción 4090541, Chile”. The authors would like to apologize for any inconvenience caused to the readers by these changes