Evaluation of pneumonitis and sialadenitis in Treg cell- and CCR2-Treg cell-transferred MRL/lpr mice

<p><b>Copyright information:</b></p><p>Taken from "Therapy for pneumonitis and sialadenitis by accumulation of CCR2-expressing CD4CD25regulatory T cells in MRL/lpr mice"</p><p>http://arthritis-research.com/content/9/1/R15</p><p>Arthritis Research & Therapy 2007;9(1):R15-R15.</p><p>Published online 7 Feb 2007</p><p>PMCID:PMC1860074.</p><p></p> The degrees of pneumonitis and sialadenitis of control and treated 17-week-old MRL/lpr mice were scored as described in Materials and methods. Values are presented as the mean and standard deviation (= 5 to 7 mice per group). Similar results were observed in two independent experiments. *< 0.01 versus control by Student's test. CCR2-Treg cell, CD4CD25Foxp3CCR2-transfected T cell; MRL/lpr, MRL/MpJ-/; Treg cell, CD4CD25Foxp3T cell

Kinetics of expression in the tissues after transfer of Treg and CCR2-Treg cells

<p><b>Copyright information:</b></p><p>Taken from "Therapy for pneumonitis and sialadenitis by accumulation of CCR2-expressing CD4CD25regulatory T cells in MRL/lpr mice"</p><p>http://arthritis-research.com/content/9/1/R15</p><p>Arthritis Research & Therapy 2007;9(1):R15-R15.</p><p>Published online 7 Feb 2007</p><p>PMCID:PMC1860074.</p><p></p> Five hundred thousand Treg or CCR2-Treg cells were injected into the retro-orbital vein of 14-week-old MRL/lpr mice. Then, the lungs and submandibular glands were harvested at 3 hours, 24 hours, 72 hours, 5 days, and 7 days after transfer. Quantitative real-time polymerase chain reaction analysis was performed on total RNA prepared from the above tissues. Results are calculated as a ratio of expression to the expression of . Analysis of the mononuclear cells in the lungs and submandibular glands of MRL/lpr mice after transfer of Treg and CCR2-Treg cells. The mononuclear cells in the lungs and submandibular glands were harvested at 3 hours and 24 hours after transfer as described in Materials and methods and analyzed by immunofluorescence on an Olympus IX70 inverted microscope. CCR2-Treg cells contain green fluorescent protein and red fluorescent protein (DsRed), whereas Treg cells contain only DsRed. Results are shown as a percentage of staining cells (Treg or CCR2-Treg cells) (= 3 or 4 mice per group). CCR2-Treg cell, CD4CD25Foxp3CCR2-transfected T cell; Foxp3, forkhead box p3; MRL/lpr, MRL/MpJ-/; Treg cell, CD4CD25Foxp3T cell

Comparison of pneumonitis and sialadenitis in Treg cell- and CCR2-Treg cell-transferred MRL/lpr mice

<p><b>Copyright information:</b></p><p>Taken from "Therapy for pneumonitis and sialadenitis by accumulation of CCR2-expressing CD4CD25regulatory T cells in MRL/lpr mice"</p><p>http://arthritis-research.com/content/9/1/R15</p><p>Arthritis Research & Therapy 2007;9(1):R15-R15.</p><p>Published online 7 Feb 2007</p><p>PMCID:PMC1860074.</p><p></p> Representative photographs are shown. Treg and CCR2-Treg cells were transferred via retro-orbital injection in 12-week-old MRL/lpr mice. One hundred thousand or 5 × 10cells were injected into mice twice every 2 weeks, and the pathological changes were evaluated 3 weeks after the last injection (that is, when the mice were 17 weeks old). Non-treated 17-week-old MRL/lpr mice were used as control. CCR2-Treg cell, CD4CD25Foxp3CCR2-transfected T cell; HE, hematoxylin and eosin; MRL/lpr, MRL/MpJ-/; Treg cell, CD4CD25Foxp3T cell

Monocyte chemoattractant protein-1 (MCP-1) expression in the lungs and submandibular glands of MRL/lpr mice

<p><b>Copyright information:</b></p><p>Taken from "Therapy for pneumonitis and sialadenitis by accumulation of CCR2-expressing CD4CD25regulatory T cells in MRL/lpr mice"</p><p>http://arthritis-research.com/content/9/1/R15</p><p>Arthritis Research & Therapy 2007;9(1):R15-R15.</p><p>Published online 7 Feb 2007</p><p>PMCID:PMC1860074.</p><p></p> Quantitative real-time polymerase chain reaction analysis was performed on total RNA prepared from lungs and submandibular glands of five mice at ages 8, 10, 12, 16, and 20 weeks during the development of pneumonitis and sialadenitis as described in Materials and methods. Results are calculated as a ratio of expression to the expression of . Representative immunohistochemistry results are shown. Formalin-fixed sections were deparaffinized and incubated with biotin-labeled goat anti-mouse MCP-1 polyclonal antibody, and the expression of MCP-1 was detected with avidin-biotin-peroxidase. The sections were counterstained with hematoxylin. MRL/lpr, MRL/MpJ-/

Prisma Groot Woordenboek Afrikaans en Nederlands

Bottom of a bed at 171.24 meters with Arenicolites

S1. Correlation of Arenicolites horizons and ratio of dolomite/calcite of the carbonate beds analyzed by XRD analysis. from Penetrative trace fossils from the late Ediacaran of Mongolia: early onset of the agronomic revolution

Correlation of Arenicolites horizons and ratio of dolomite/calcite of the carbonate beds analyzed by XRD analysis. Note that most of the Arenicolites horizons are located in the limestone, not in heavily dolomitized parts

Serum levels of anti-RRP8 (A) and anti-TNP1 (B) antibodies in patients with rheumatic diseases.

<p>Cut-off values for positivity (9.1 units for anti-RRP8 antibody and 10.6 units for anti-TNP1 antibody) are indicated by the dotted lines. LN, lupus nephrits; SLE, systemic lupus erythematosus without LN; DM, dermatomyositis; PM, polymyositis; SSc, systemic sclerosis; MCTD, mixed connective tissue disease; RA, rheumatoid arthritis; SS, Sjögren syndrome; BD, Behçet’s disease; AAV, anti-neutrophil cytoplasmic antibody-associated vasculitis.</p

Relationship between anti-RRP8 or anti-TNP1 antibody and clinical findings in SLE patients.

<p><b>(A)</b> anti-RRP8 antibody and anti-dsDNA antibodies, <b>(B)</b> Anti-RRP8 antibody and SLEDAI, <b>(C)</b> anti-TNP1 antibody and anti-dsDNA antibodies, <b>(D)</b> anti-TNP1 antibody and SLEDAI. SLEDAI, systemic lupus erythematosus disease activity index. Correlations were expressed as Spearman rank correlation coefficients. P values of <0.05 were considered significant.</p

Screening of 17 autoantigens associated with LN using a biotinylated human autoantigen library.

<p>Luminescence signal was calculated as the signals/noise ratio. Expected isoelectric point was estimated using an online calculator (<a href="http://isoelectric.ovh.org/" target="_blank">http://isoelectric.ovh.org/</a>).</p><p>Screening of 17 autoantigens associated with LN using a biotinylated human autoantigen library.</p

Double immunofluorescence of RRP8 or TNP1 and IgG or C3 in renal sections from patients with LN.

<p>Formalin-fixed, paraffin-embedded sections of biopsy or autopsy specimens from LN patients were processed for double immunofluorescence staining. Representative photographs are shown. The specimens were stained with Alexa 546-conjugated anti-RRP8 or anti-TNP1 antibodies (red) and with FITC-conjugated anti-IgG or anti-C3 antibodies (green). <b>(A)</b> The same glomeruli were stained with periodic acid-Schiff. <b>(B)</b> Co-presence of RRP8 and C3 was detected along the sub-epithelial area of the glomeruli showing a dotted pattern (arrowhead) in LN1. Also in Autopsy1, both RRP8 and IgG signals were detected in the mesangial area as well as in the sub-epithelial area (arrowhead). <b>(C)</b> Co-presence of TNP1 and IgG was revealed along the basement membrane showing a linear pattern in LN2, as was the case for TNP1 and C3 in Autopsy2. The bold and strong autofluorescence in each of the panels is mainly due to red blood cells.</p