<p><b>Copyright information:</b></p><p>Taken from "Therapy for pneumonitis and sialadenitis by accumulation of CCR2-expressing CD4CD25regulatory T cells in MRL/lpr mice"</p><p>http://arthritis-research.com/content/9/1/R15</p><p>Arthritis Research & Therapy 2007;9(1):R15-R15.</p><p>Published online 7 Feb 2007</p><p>PMCID:PMC1860074.</p><p></p> Quantitative real-time polymerase chain reaction analysis was performed on total RNA prepared from lungs and submandibular glands of five mice at ages 8, 10, 12, 16, and 20 weeks during the development of pneumonitis and sialadenitis as described in Materials and methods. Results are calculated as a ratio of expression to the expression of . Representative immunohistochemistry results are shown. Formalin-fixed sections were deparaffinized and incubated with biotin-labeled goat anti-mouse MCP-1 polyclonal antibody, and the expression of MCP-1 was detected with avidin-biotin-peroxidase. The sections were counterstained with hematoxylin. MRL/lpr, MRL/MpJ-/
