Mutations other than 103N in human immunodeficiency virus type 1 reverse transcriptase (RT) emerge from K103R polymorphism under non-nucleoside RT inhibitor pressure

AbstractK103N mutation in human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) confers high-level resistance against non-nucleoside RT inhibitors (NNRTIs) and it easily occurs partly because it arises by a single nucleotide substitution from wild-type K103. There are polymorphisms at codon 103 of HIV-1 RT. We found K103R polymorphic mutation in 3.3% of treatment-naive HIV-1-infected patients. R103N does not seem to occur as easily as K103N because R103N requires two nucleotide substitutions. To induce NNRTI resistance-associated mutations, HIV-1K103R was propagated in the presence of increasing concentrations of efavirez (EFV) or nevirapine (NVP). V179D emerged in all three EFV cultures and in two of four NVP cultures. R103G emerged by a single nucleotide substitution in one of three EFV cultures. R103N did not emerge in any of 7 NNRTI cultures. Analysis of recombinant HIV-1s showed that HIV-1K103R/V179D was significantly resistant and HIV-1K103G was moderately resistant against EFV and NVP

Pseudoacromegaly with acromegalic features in radiography

Pseudoacromegaly is a condition characterized by acromegalic physical features without growth hormone excess, for which radiographic observation has seldom been reported. This is a rare case of pseudoacromegaly

タンパク質間相互作用ネットワークからシグナル伝達パスウェイを検出する手法の開発

We developed a new method to searchfunctionally similar protein interaction networks witha glVen query Slgnal transduction pathway from protein interaction networks.This method consists of two main parts: 1)a backtracking search algorithm to find topologically identical subgraphs and 2) a measurement of similaritybetween proteins by using Gene Ontology [1]. For validation of our method, we implemented a software tool and compared its performancewiththat of PathBLAST [2] onthe search of MAPK signal transduction cascade[3]. The result showed that our software tool detects correct answers with low false-positive rate. This tool wi11 provide better performance when combined with computational method of predicting proteinfunctions

変形性膝関節症単純X線画像における重症度自動定量評価システムの開発

学位の種別: 論文博士審査委員会委員 : （主査）東京大学教授 芳賀 信彦, 東京大学講師 門野 夕峰, 東京大学特任准教授 林 直人, 東京大学准教授 小川 純人, 東京大学講師 有田 淳一University of Tokyo(東京大学

A Sensorimotor Signature of the Transition to Conscious Social Perception: Co-regulation of Active and Passive Touch

It is not yet well understood how we become conscious of the presence of other people as being other subjects in their own right. Developmental and phenomenological approaches are converging on a relational hypothesis: my perception of a “you” is primarily constituted by another subject’s attention being directed toward “me.” This is particularly the case when my body is being physically explored in an intentional manner. We set out to characterize the sensorimotor signature of the transition to being aware of the other by re-analyzing time series of embodied interactions between pairs of adults (recorded during a “perceptual crossing” experiment). Measures of turn-taking and movement synchrony were used to quantify social coordination, and transfer entropy was used to quantify direction of influence. We found that the transition leading to one’s conscious perception of the other’s presence was indeed characterized by a significant increase in one’s passive reception of the other’s tactile stimulations. Unexpectedly, one’s clear experience of such passive touch was consistently followed by a switch to active touching of the other, while the other correspondingly became more passive, which suggests that this intersubjective experience was reciprocally co-regulated by both participants

Distinct associations of different sedentary behaviors with health-related attributes among older adults

Objective: Leisure-time sedentary behaviors (LTSBs) have been associated adversely with health outcomes. However, limited research has focused on different categories of LTSB. We aimed at identifying categories of LTSBs and examining their separate associations with indices of health among Japanese older adults. Methods: A postal survey collected data on self-reported health, psychological distress, body mass index, moderate-to-vigorous physical activity (MVPA), LTSBs (five behaviors) and socio-demographic characteristics from 1,580 Japanese older adults (67% response rate; 65-74 years) in 2010. Exploratory factor analysis was used to classify LTSBs. Odds ratios (ORs) were calculated for associations of LTSB categories with self-reported health, psychological distress, overweight, and lower MVPA. Data were analyzed in 2013. Results: Two categories of LTSB: passive sedentary time (consisting of TV time, listening or talking while sitting, and sitting around) and mentally-active sedentary time (consisting of computer-use and reading books or newspapers) were identified. Higher passive sedentary time was associated with a higher odds of being overweight (OR: 1.39, [95% CI: 1.08-1.80]), and lower MVPA (1.26, [1.02-1.54]). Higher mentally-active sedentary time was associated with lower odds of lower MVPA (0.70, [0.57-0.86]). Conclusions: Two types of sedentary time-passive and mentally-active-may play different roles in older adults' well-being

Eosinophilic Gastritis in a Patient Previously Treated with Dupilumab

A 77-year-old Japanese man with bronchial asthma was treated with dupilumab. Dupilumab treatment was discontinued at the patient's request after two injections separated by a 2-week interval. The blood eosinophil count was elevated, and an esophagogastroduodenoscopy performed 3 months after dupilumab treatment revealed gastric ulcers; subsequently, eosinophilic gastritis was diagnosed from biopsy examinations. The gastric lesions were resolved by steroid administration. This case report underscores that eosinophil-associated gastrointestinal diseases should be considered in the differential diagnosis of gastric lesions occurring in patients who were treated with dupilumab

Laboratory changes during adrenocorticotropic hormone therapy associated with renal calcified lesions

Background Renal calcified lesions are known as one of the complications during adrenocorticotropic hormone (ACTH) therapy for intractable epilepsy. However, laboratory changes during the therapy or laboratory features of high‐risk cases with renal calcified lesions are yet to be clarified. Methods In this study, 43 patients with West syndrome aged ≤2 years were included. We retrospectively reviewed age and body mass index at the beginning of ACTH therapy, as well as the amount of fluid intake, daily urinary volume, and laboratory data during therapy. In addition, we studied the urinary sediment of the cases with renal calcified lesions diagnosed by computed tomography. Results After initiating ACTH treatment, urinary calcium (Ca)/creatinine ratio and urinary pH increased within 2 weeks. Urinary crystals and renal tubular epithelial cells (RTECs) in urinary sediment were frequently found in most cases. Urinary Ca levels, proteinuria or frequency of urinary crystals, and number of RTECs in the urinary sediment were significantly higher in patients with epithelial casts (ECs) or hematuria than in patients without these findings. Among the seven patients who underwent abdominal CT, ECs or hematuria were found only in those with renal calcified lesions. These findings suggested that patients with ECs or hematuria were more likely to have calcified lesions. Conclusions The risk of renal calcified lesions increased after 2 weeks of ACTH treatment. Abnormal findings in urinary sediments might be an early sign of renal calcification during ACTH therapy

Local lattice instability and stripes in the CuO 2 plane of the La 1.85 Sr 0.15 CuO 4 system by polarized XANES and EXAFS

Temperature-dependent polarized Cu K-edge x-ray absorption has been used to investigate local structural distortions in the ${\mathrm{CuO}}_{2}$ plane of the ${\mathrm{La}}_{1.85}$${\mathrm{Sr}}_{0.15}$${\mathrm{CuO}}_{4}$ system. The Cu-O pair distribution shows the presence of a minority phase containing ${\mathrm{CuO}}_{6}$ octahedra characterized by a shorter Cu-O(apical) bond (\ensuremath{\Delta}R\ensuremath{\sim}-0.1 \AA{}) and two longer Cu-O(planar) bonds (\ensuremath{\Delta}R\ensuremath{\sim}+0.08 \AA{}) and a tilting angle of 16\ifmmode^\circ\else\textdegree\fi{}\ifmmode\pm\else\textpm\fi{}2\ifmmode^\circ\else\textdegree\fi{}. The temperature-dependent distortions show a maximum around T (\ensuremath{\sim}1.${6\mathrm{T}}_{\mathrm{c}}$) and a minimum at ${\mathrm{T}}_{\mathrm{c}}$ (\ensuremath{\sim}35 K). The data show the coexistence of two types of doped charges in different stripes in the superconducting phase