Pengaruh Komunikasi Terapeutik Perawat Terhadap Kepuasan Pasien Di Rawat Jalan RSUD Jogja

The Objective of this study is to know influence of nurse therapeutic communication to satisfaction of patients satisfaction in RSUD Yogyakarta. The study was a quantitative research methods such as surveys of descriptive inferential research with cross sectional approach. Number of samples in this research is 285 sample in inpatient and 140 in emergency room. The instrument used a questionnaire. Analysis of data using multiple linear regression. This study show that there is the influence of therapeutic communication nurse to satisfaction of outpatients and Emergency room in RSUD Yogyakarta, and orientation phase is a phase that most influence on patient satisfaction. The most influential to therapeutic communication is termination stage

Bioactive Maleic Anhydrides and Related Diacids from the Aquatic Hyphomycete <i>Tricladium castaneicola</i>

Four maleic anhydride derivatives, tricladolides A–D (<b>1</b>–<b>4</b>), and three alkylidene succinic acid derivatives, tricladic acids A–C (<b>5</b>–<b>7</b>), were isolated from the aquatic hyphomycete <i>Tricladium castaneicola</i>. The structures of these compounds were determined by spectroscopic analysis, and all were found to be novel. The compounds exhibited inhibitory activity against fungi, particularly <i>Phytophthora</i> sp., a plant pathogen of oomycetes. The inhibitory activity of these metabolites revealed the importance of the cyclic anhydride structure and the lipophilicity of the alkyl side chain. On the other hand, the cytotoxicity of the compounds against B16 melanoma cells indicated that the cyclic anhydride structure was not essential

The ultrastructural characteristics of porcine hepatocytes donated after cardiac death and preserved with warm machine perfusion preservation

<div><p>The effects of warm machine perfusion preservation of liver grafts donated after cardiac death on the intracellular three-dimensional ultrastructure of the organelles in hepatocytes remain unclear. Here we analyzed comparatively the ultrastructure of the endomembrane systems in porcine hepatocytes under warm ischemia and successive hypothermic and midthermic machine perfusion preservation, a type of the warm machine perfusion. Porcine liver grafts which had a warm ischemia time of 60 minutes were perfused for 4 hours with modified University of Wisconsin gluconate solution. Group A grafts were preserved with hypothermic machine perfusion preservation at 8°C constantly for 4 hours. Group B grafts were preserved with rewarming up to 22°C by warm machine perfusion preservation for 4 hours. An analysis of hepatocytes after 60 minutes of warm ischemia by scanning electron microscope revealed the appearance of abnormal vacuoles and invagination of mitochondria. In the hepatocytes preserved by subsequent hypothermic machine perfusion preservation, strongly swollen mitochondria were observed. In contrast, the warm machine perfusion preservation could preserve the functional appearance of mitochondria in hepatocytes. Furthermore, abundant vacuoles and membranous structures sequestrating cellular organelles like autophagic vacuoles were frequently observed in hepatocytes after warm machine perfusion preservation. In conclusion, the ultrastructure of the endomembrane systems in the hepatocytes of liver grafts changed in accordance with the temperature conditions of machine perfusion preservation. In addition, temperature condition of the machine perfusion preservation may also affect the condition of the hepatic graft attributed to autophagy systems, and consequently alleviate the damage of the hepatocytes.</p></div

The ultrastructural alteration in porcine hepatocytes preserved by HMP.

<p>(A) Representative hepatocytes were observed by SEM in the osmium-macerated porcine liver graft samples preserved by HMP for 4 h after 60 minutes of warm ischemia. Nucleus was colored blue and abnormal vacuoles were colored red. The partial area indicated in A was further photographed at a higher magnification (B). Mitochondria were colored green and abnormal vacuoles were colored red. Bars = 1 μm.</p

The ultrastructural characteristics in porcine hepatocytes preserved by MMP.

<p>(A) Representative hepatocytes were observed by SEM in the osmium-macerated porcine liver graft samples preserved by MMP for 4 h after 60 minutes of warm ischemia. Nucleus was colored blue and abnormal vacuoles were colored red. Asterisks indicated the vacuoles include the vesicles and mitochondria. The partial area indicated in A was further photographed at a higher magnification (B). Abnormal vacuoles were colored red. Cytoplasmic mitochondria were colored green and intravacuolar mitochondria were colored purple. (C) Mitochondria areas were measured in SEM images (9–11 views of each groups, N = 3). Data represents as the means ± SEM. Unpaired two-tailed t-tests were used (p < 0.0001). Bars = 1 am.</p

The ultrastructure of the endomembrane structure in the hepatocytes of the control liver.

<p>(A and C) Typical hepatocytes were identified in the ultrathin sections of the Epon 812-embedded control liver tissue (A). Nucleus was colored blue. The partial area indicated in A was further photographed at a higher magnification (C). Nucleus was colored blue and mitochondria were colored green. Bars = 1 μm. (B and D) Similar typical hepatocytes in osmium-macerated control porcine liver tissues were viewed with a scanning electron microscope (B). Nucleus was colored blue. The p area indicated in B was further photographed at a higher magnification (D). Nucleus was colored blue and mitochondria were colored green. Open arrows indicated cristae of mitochondria. Bars = 1 μm.</p

Schematic representation of the continuous machine perfusion system.

<p>Schematic representation of the continuous machine perfusion system.</p

Additional file 4: Figure S3. of Predictive biomarkers for the efficacy of peptide vaccine treatment: based on the results of a phase II study on advanced pancreatic cancer

Frequency of CD4+ CD45RA- CD25high cells and CD11b + CD33+ cells. (a), (c) There were no differences in the percentages of CD4+ CD45RA- CD25high cells and CD11b + CD33+ cells in the 46 patients before and after treatment. (b), (e) Before and after treatment, there were no differences in the percentages of CD4+ CD45RA- CD25high cells and CD11b + CD33+ cells between the patients with a long survival (n = 6) and the patients with a short survival (n = 19) in the HLA-A*2402-matched group. (c), (f) Before and after treatment, there were no differences in the percentages of CD4+ CD45RA- CD25high cells and CD11b + CD33+ cells between the patients with a long survival (n = 6) and the patients with a short survival (n = 15) in the HLA-A*2402-unmatched group. (TIF 60 kb

Additional file 2: Table S1. of Predictive biomarkers for the efficacy of peptide vaccine treatment: based on the results of a phase II study on advanced pancreatic cancer

Comparison of prognostic factors according to the numbers of peptide-specific responses (n = 36 HLA-A*2402-matched patients). (DOCX 28 kb