134 research outputs found
Introgression and Long-Term Naturalization of Archaeophytes into Native Plants - Underestimated Risk of Hybrids
The expression of PI3K/Akt/mTOR signaling pathway in early-stage small cell carcinoma of the uterine cervix
Femtosecond X-ray emission study of the spin cross-over dynamics in haem proteins
In haemoglobin (consisting of four globular myoglobin-like subunits), the
change from the low-spin (LS) hexacoordinated haem to the high spin (HS)
pentacoordinated domed form upon ligand detachment and the reverse process upon
ligand binding, represent the transition states that ultimately drive the
respiratory function. Visible-ultraviolet light has long been used to mimic the
ligand release from the haem by photodissociation, while its recombination was
monitored using time-resolved infrared to ultraviolet spectroscopic tools.
However, these are neither element- nor spin-sensitive. Here we investigate the
transition state in the case of Myoglobin-NO (MbNO) using femtosecond Fe Kalpha
and Kbeta non-resonant X-ray emission spectroscopy (XES) at an X-ray
free-electron laser upon photolysis of the Fe-NO bond. We find that the
photoinduced change from the LS (S = 1/2) MbNO to the HS (S = 2)
deoxy-myoglobin (deoxyMb) haem occurs in ca. 800 fs, and that it proceeds via
an intermediate (S = 1) spin state. The XES observables also show that upon NO
recombination to deoxyMb, the return to the planar MbNO ground state is an
electronic relaxation from HS to LS taking place in ca. 30 ps. Thus, the entire
ligand dissociation-recombination cycle in MbNO is a spin cross-over followed
by a reverse spin cross-over process
Femtosecond time-resolved X-ray absorption spectroscopy of anatase TiO2 nanoparticles using XFEL
光触媒ナノ粒子における光照射後10兆分の1秒での電子の動きをX線自由電子レーザーで観測. 京都大学プレスリリース. 2017-07-06.The charge-carrier dynamics of anatase TiO2 nanoparticles in an aqueous solution were studied by femtosecond time-resolved X-ray absorption spectroscopy using an X-ray free electron laser in combination with a synchronized ultraviolet femtosecond laser (268 nm). Using an arrival time monitor for the X-ray pulses, we obtained a temporal resolution of 170 fs. The transient X-ray absorption spectra revealed an ultrafast Ti K-edge shift and a subsequent growth of a pre-edge structure. The edge shift occurred in ca. 100 fs and is ascribed to reduction of Ti by localization of generated conduction band electrons into shallow traps of self-trapped polarons or deep traps at penta-coordinate Ti sites. Growth of the pre-edge feature and reduction of the above-edge peak intensity occur with similar time constants of 300–400 fs, which we assign to the structural distortion dynamics near the surface
Two types of antigorite serpentinite controlling heterogeneous slow-slip behaviours of slab–mantle interface
The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force
「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection
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