Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors

This research was originally published in the Journal of Biological Chemistry. F Oyama, S Hirohashi, Y Shimosato, K Titani and K Sekiguchi. Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors. J. Biol. Chem. 1989; 264: 10331-10334 © the American Society for Biochemistry and Molecular Biolog

Genome-wide DNA methylation profiles in both precancerous conditions and clear cell renal cell carcinomas are correlated with malignant potential and patient outcome

To clarify genome-wide DNA methylation profiles during multistage renal carcinogenesis, bacterial artificial chromosome array-based methylated CpG island amplification (BAMCA) was performed. Non-cancerous renal cortex tissue obtained from patients with clear cell renal cell carcinomas (RCCs) (N) was at the precancerous stage where DNA hypomethylation and DNA hypermethylation on multiple bacterial artificial chromosome (BAC) clones were observed. By unsupervised hierarchical clustering analysis based on BAMCA data for their N, 51 patients with clear cell RCCs were clustered into two subclasses, Clusters AN (n = 46) and BN (n = 5). Clinicopathologically aggressive clear cell RCCs were accumulated in Cluster BN, and the overall survival rate of patients in Cluster BN was significantly lower than that of patients in Cluster AN. By unsupervised hierarchical clustering analysis based on BAMCA data for their RCCs, 51 patients were clustered into two subclasses, Clusters AT (n = 43) and BT (n = 8). Clinicopathologically aggressive clear cell RCCs were accumulated in Cluster BT, and the overall survival rate of patients in Cluster BT was significantly lower than that of patients in Cluster AT. Multivariate analysis revealed that belonging to Cluster BT was an independent predictor of recurrence. Cluster BN was completely included in Cluster BT, and the majority of the BAC clones that significantly discriminated Cluster BN from Cluster AN also discriminated Cluster BT from Cluster AT. In individual patients, DNA methylation status in N was basically inherited by the corresponding clear cell RCC. DNA methylation alterations in the precancerous stage may generate more malignant clear cell RCCs and determine patient outcome

Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors

This research was originally published in the Journal of Biological Chemistry. F Oyama, S Hirohashi, Y Shimosato, K Titani and K Sekiguchi. Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors. J. Biol. Chem. 1989; 264: 10331-10334 © the American Society for Biochemistry and Molecular Biolog

INCREASED EXPRESSION OF c-myc GENE

High levels of c-myc mRNA were observed in two human tumor cell lines, a giant cell carcinoma of the lung (C-Lu99) and a colon cancer (C1). The increased expression of c-myc in these cell lines, which was comparable with those in cell lines in which the c-myc gene is amplified, was not due to gene amplification. Run-on transcription revealed that the transcriptional rate of the c-myc gene was greatly increased in these cell lines