Influencing Factors and Mechanism for Morphological Alterations of Aponogeton crispus and Their Genetic Comparison at the Dediyagala Stream, Sri Lanka

Streams are one of the main aquatic ecosystems which have a connectivity form head waters to oceans. Along this longitudinal gradient, streams exhibit huge faunal and floral diversity. Despite the well-observed variations with in the same floral species along the streams; information on factors behind such variations is scarce to our knowledge. Thus, the present study was designed to study the factors behind the observed morphological variations in Aponogeton crispus (Kekatiya in Sinhala) found in the Dediyagala stream located in Matara district. Five study sites were selected along a segment (~2 km) of the stream. For each site, plant density was detected in randomly selected quadrate (50×50 cm2) and plants were sampled for the subsequent morphological, anatomical, biochemical and genetic analysis. Physichochemical properties such as pH, temperature, and dissolved oxygen content, water flow velocity and conductivity of water were measured onsite while nutrients (NO3- and PO43- concentration in water and soil) was measured at the laboratory. Sediment core samples were brought to detect organic matter content and the particle size distribution. Molecular identification of selected plant samples was performed by DNA barcoding. Morphological variations such as length, width folds of plant leaves were observed and they significantly correlated to the abiotic factors including velocity, plant density, particle size, soil nitrate, water nitrate, dissolved oxygen, pH, and total suspended solids. However, irrespective of the observed morphological variations in the selected plants, the molecular composition of the DNA barcoding regions namely, MatK and psbA-trnH of the selected plants were found to be the same as wild type plant Apanogetton crispus. This was further confirmed by the phylogenetic tree analysis. Therefore, this study revealed that the morphological variations in Aponogeton crispus can be attributed to the function of abiotic factors. Together with these finding further studies on other factors that may influence the morphology variance and DNA barcode for another primers could assist better understanding and management of aquatic ecosystem.Keywords: Aponogeton crispus, Morphology, DNA barcoding, Aquatic macrophyte

Growth characteristics and meat quality atributes of Clam (Geloina vexans) and Oysters (Crassostrea madrasensis) harvested from two lagoons of Sri Lanka.

772-777The present study was focused to evaluate nutritional quality parameters, Ecophysiological and commercial quality indicators of clam (Geloina vexans) and oyster (Crassostrea madrasensis) at two growth stages (4 months and 6 months) in two different Sri Lankan sites, namely Bolgoda lagoon in Moratuwa area and Pitipana lagoon in Negombo area. According to the results of this study total saturated fatty acids (34.4%) as well as total mono saturated fatty acids are high in clams of Bolgoda lagoon. However, total polyunsaturated fatty acids are high in clams of Negambo lagoon. Results revealed that crude protein level is significantly (p<0.05) higher in both species grown in Bolgoda lagoon (CP% 70.8±1.42g/100g and 48.97±2.38g/100g clams in Bolgoda and Negombo respectively while CP% 34.12+1.18 and 45.2+2.23g/100g oysters in Bolgoda and Negambo respectively). However intervalval fluid contents of 6 months age clams and oysters in Negombo are significantly higher than Bolgoda oysters (P<0.05) which are 11.39±1.68 g/100g and 9.11± 1.68 g/100g respectively. Water salinity, temperature and pH are some values which are significantly higher in Negombo lagoon than Bolgoda (P<0.05) while dissolved oxygen and resistivity are significantly higher in Bolgoda than Negombo. Results revealed that availability of planktons is high in Bolgoda lagoon. And this may be the possible reason for high CP, ash, DM and meat content in clams and oysters of Bolgoda lagoon. Rich lagoon conditions in Bolgoda lagoon has caused to the high nutritional value and high demand of bivalves in Bolgoda lagoon in Sri Lanka