Removal of nutrients (N and P) and heavy metals (Fe, Al, Mn and Ni) from industrial wastewaters by phytoremediation using water hyacinth (Eichhornia crassipes) under different nutritional conditions

This study was investigated the utilization of phytoremediation strategies to remove nitrogen, phosphorus and heavy metals (Fe, Al, M n and Ni) from wastewaters by water hyacinth {Eichhornia crassipes [Mart.] Solms). Batch studies were conducted for 15 weeks using fiberglass tanks in which healthy young plants were grown for a period of 15 weeks under different nutrient concentrations o f 2-fold (56 T N mg/1 a nd 15.4 T P mg/1), 1-fold (28 T N mg/1 a n d 7.7 T P mg/1), 1/2-fold (14 T N mg/1 a n d 3.85 TP mg/1), 1/4-fold (7 T N mg/1 a n d 1.93 T P mg/1), 1/8-fold (3.5 T N mg/1 a n d 0.96 TP mg/1) and control (without nutrients). In each week plants, water and sediments were analysed for TN and TP. The phytoremediation potential of heavy metal removal was determined at above nutrient concentrations with the addition of the constant heavy metal concentrations (Fe-9.27mg/1, Al-5.62mg/1, Mn- 0.92 mg/1and Ni-0.21mg/1) in fiberglass tanks. Plant, water and sediment were analyzed for heavy metals during the 15 weeks of culture period. A mass balance was conducted to investigate the phytoremediation efficiencies and to determine the different mechanisms governing nutrient and heavy metal removal from the wastewaters. Our results manifested that hyacinth could be effectively utilized in constructed wetlands to phytoremediate N rich wastewaters than P. Plant uptake was the major TN and TP removal mechanism during the initial periods. Accumulation of a high content of nitrogen in plant tissues due to plant uptake and denitrification was found to be the key mechanisms involved in the efficient removal of nitrogen at the latter part of the study period. Plant uptake of phosphorus and chemical precipitation together with adsorption on to the detritus are the key mechanisms of phosphorus removal. However the phosphorus removal seems to be not high with that of nitrogen indicating that hyacinth systems are not ideal for phosphorus removal from wastewaters. In conclusion, very young plants having seems to be ideal to commence a constructed wetland after a period of acclimatization and approximately 56-63 days of hydraulic retention time is recommended for optimum phytoremediation of nitrogen as well as phosphorus. Phytoremediation of Fe largely due to the process of rhizofiltration and the chemical precipitation followed by flocculation and sedimentation were the key Fe removal mechanisms during the first few weeks of the study. Plants grown in the control set-up showed a highest phytoremediation efficiency of 47% during optimum growth at the 6th week with a highest accumulation of 6707 Fe mg/kg dry weight. Root effluxing of Fe to the waste waters at intermittent periods and with time was a key mechanism of avoiding Fe phytotoxicity in water hyacinth. It can be concluded from this study that water hyacinth is an ideal plant for a batch removal of low polluting Fe rich industrial wastewaters under completely nutrient poor conditions. Very young plants are ideal to commence a constructed wet land after a period of acclimatization and approximately 42 days hydraulic retention time is recommended for optimum phytoremediation. Phytoextraction was the key Mn removal mechanism and root effluxing of Mn was observed intermittently possibly to avoid any phytotoxicity caused by an excessive accumulation of Mn in hyacinths. Hyacinths cultured in the 1/8-fold set-up showed a highest accumulation of 1133 Mn mg/kg dry weight with an optimum removal of 79% at the 9th week. Hence very young plants inhabiting waterbodies containing approximately 3.5 TN mg/1 a n d 0.96 TP mg/1 seems to be more ideal for a batch removal of low polluting Mn rich wastewaters in constructed wetlands. Acclimatization of the plants is necessary for at least 1 week prior to the removal of Mn and then approximately 63 days hydraulic retention time is recommended to optimize phytoremediation. Chemical precipitation followed by flocculation and sedimentation with phytoremediation mainly due to rhizofiltration were the key Al removal mechanisms Control and 1/8-fold set-ups showed higher phytoremediation efficiencies of 63% and 54%, respectively with maximum accumulations of 4278 Al mg/kg dry weight and 4224 Al mg/kg dry weight, respectively. Therefore young plants of completely nutrient starved adult hyacinths seems to be more ideal for a batch removal of low polluting Al rich industrial wastewaters in pilot scale constructed wetlands. A hydraulic retention time of approximately 28 days is recommended for optimum removal after a period of acclimatization of the young plants. The results manifested that hyacinths are essentially Ni excluders since higher levels of Ni were detected in water throughout the study

Histological analysis of plant regeneration from plumule explants of Cocos nucifera

Plant regeneration was achieved from plumules excised from mature zygotic embryos of a local coconut cultivar (Sri Lanka Tall). A detailed histological study was undertaken to gain a better understanding of the cellular changes that occur during plant regeneration from plumule tissues. This study led to the identification of the cellular origin, specific cell characterization and development pattern of embryogenic calluses. It also revealed that abscisic acid induces plant regeneration through somatic embryogenesis. The presence of incomplete somatic embryos that lacked shoot poles was also observed. (Résumé d'auteur

Removal of nitrogen and phosphorus from wastewaters by phytoremediation using water hyacinth ieichhornia crassipiesy

Water hyacinth (Eichhomia crassipes [Mart.] Solms.) is one of the most prominent freefloating aquatic weeds found throughout the tropical and subtropical areas of the world. In Sri Lanka the water hyacinth was first introduced in 1904 to the Botanical Gardens. Since then it has been recognized as one of the most troublesome aquatic weeds in many freshwater bodies. However it has been documented that water hyacinth has a unique ability to remove nutrients and heavy metals from polluted waters