Orientations fonctionnelles de cellules adhérentes (mélanomes B16 et préostéoblastes MCT3) cultivées sur un support cellulosique

Les biomatériaux, en fonction de leur nature et de leurs propriétés de surface, peuvent donner aux cellules placées à leur contact, différentes morphologies qui seront déterminantes pour leurs orientations fonctionnelles. L'objectif de cette thèse est l'étude des réactions de cellules cultivées au contact d'un support original pour culture cellulaire. Les lignées de cellules adhérentes utilisées lors de cette étude ont été trois lignées des cellules de mélanomes murins B16 de pouvoir métastatique croissant et des préostéoblastes murins MC3T3. Les principaux résultats obtenus sont les suivants : une morphologie cellulaire arrondie et une agrégation des cellules ; une inhibition de la prolifération cellulaire ; une induction de la différenciation et/ou de l'apoptose. Ce revêtement nous a permis d'obtenir des résultats significatifs et reproductibles qui ouvrent la perspective de son utilisation comme outil de recherche fondamentale et d'aide au diagnostic de malignité.Biomaterials according to their nature and their surface properties can give to cells in their contact, different morphologies which will be determinant to their functional orientations. This thesis airn is to study the reactions of cells cultivated on an original coating for cell culture. Adherent cells lines used during this study are three cells lines of murine melanoma cells B 16 with growing metastatic power and MC3T3 preosteoblasts. Principal results obtained are : a cellular morphology change which is manifested by cells aggregation ; a cellular proliferation inhibition ; a differentiation induction and/or apoptosis This coating permits us to obtain significant and reproducible results which open new perspectives for its utilization as a fundamental research tool and as a malignity diagnostic help tool.COMPIEGNE-BU (601592101) / SudocSudocFranceF

Interaction of cell culture with composition effects on the mechanical properties of polycaprolactone-hydroxyapatite scaffolds fabricated via selective laser sintering (SLS)

In the current study PCL/HA composites were fabricated using SLS as two- and three-dimensional lattice structures and exposed to a cellular component (MC 313 osteoblast-like cells). The main aims were to determine the mechanical differences due to powder composition and to observe the physical and mechanical changes pertaining to cell presence. These structures were characterized by compressive mechanical testing, and the effects of cell culturing and degradation on mechanical properties of the scaffolds with different PCL/HA compositions were determined. Moreover, changes in the scaffold morphology due to the cell culture conditions were determined by mu-CF analysis.Cells steadily grew on the scaffolds for 21 days with preferential distribution around the macropores and initially PCL/HA(15%) composites had higher cell numbers. Removal of loosely sintered parts was observable during the culturing period. Cell culture conditions did not change the compressive moduli significantly but had a distinct effect on compressive strength. For PCL/HA(15%) composites, an initial loss in strength caused by cell culture was reversed by longer cell exposure, with compressive strength of the structures restored to the initial properties (p <= 0.05). mu-CT measurements showed widespread morphological changes in the scaffolds, such as a decrease in the roughness of the struts. In general, in the initial period composites with lower HA content (15 wt.%) showed better metabolic activity compared to the higher HA content, however by day 14 the performance of the two compositions was equal. These results suggest that changes in sintering due to the differences in powder composition can have profound effects on the short and long term mechanical properties of the scaffold particularly:under cell culture conditions, and this should be closely considered for SLS processing of scaffolds. (C) 2012 Elsevier B.V. All rights reserved.peer-reviewe

Interaction of cell culture with composition effects on the mechanical properties of polycaprolactone-hydroxyapatite scaffolds fabricated via selective laser sintering (SLS)

In the current study PCL/HA composites were fabricated using SLS as two- and three-dimensional lattice structures and exposed to a cellular component (MC 313 osteoblast-like cells). The main aims were to determine the mechanical differences due to powder composition and to observe the physical and mechanical changes pertaining to cell presence. These structures were characterized by compressive mechanical testing, and the effects of cell culturing and degradation on mechanical properties of the scaffolds with different PCL/HA compositions were determined. Moreover, changes in the scaffold morphology due to the cell culture conditions were determined by mu-CF analysis.Cells steadily grew on the scaffolds for 21 days with preferential distribution around the macropores and initially PCL/HA(15%) composites had higher cell numbers. Removal of loosely sintered parts was observable during the culturing period. Cell culture conditions did not change the compressive moduli significantly but had a distinct effect on compressive strength. For PCL/HA(15%) composites, an initial loss in strength caused by cell culture was reversed by longer cell exposure, with compressive strength of the structures restored to the initial properties (p <= 0.05). mu-CT measurements showed widespread morphological changes in the scaffolds, such as a decrease in the roughness of the struts. In general, in the initial period composites with lower HA content (15 wt.%) showed better metabolic activity compared to the higher HA content, however by day 14 the performance of the two compositions was equal. These results suggest that changes in sintering due to the differences in powder composition can have profound effects on the short and long term mechanical properties of the scaffold particularly:under cell culture conditions, and this should be closely considered for SLS processing of scaffolds. (C) 2012 Elsevier B.V. All rights reserved

Effects of Fibronectin Coating on Bacterial and Osteoblast Progenitor Cells Adherence in a Co-culture Assay

Bacterial adherence to the surface of implants functionalized with cell-adhesive biomolecules is a critical first step of infection development. This study was designed to determine how the immobilization of human plasmatic fibronectin (pFN) could impact bacterial and osteoblast cells interaction with the surface during concomitant exposition to the two cell-types. Calibrated suspensions of P. aeruginosa PAOI or S. aureus CIP4.83 bacteria and STRO-1+A osteoblast progenitor cells were mixed, co-seeded on glass coverslips coated or not with pFN and incubated at 37 degrees C. After 3 h of co-culture, the presence of bacteria did not modify the STRO-1+A cells adherence to glass. pFN coating significantly enhanced STRO-1+A cells, CIP4.83 and PAOI adherence to glass and bacterial interaction with STRO-1+A cells. Confocal laser scanning microscopy observations revealed that cells on the pFN-coated substrate exhibited a greater spreading, better organized network of cytoskeletal filaments, and an increased cellular FN expression than cells on the uncoated substrate. The use of fluorescently labeled pFN showed that adherent STRO-1+A cells were able to remodel and to concentrate coated pFN at the cells surface. Thus, the use of FN coating could increase the risk of bacterial adherence to the material surface, acting either directly onto the coating layer or indirectly on adherent osteoblastic cells. This may increase the infection risk in the presence of bacterial contaminatio

Characterization of Breast Implant Surfaces, Shapes, and Biomechanics: A Comparison of High Cohesive Anatomically Shaped Textured Silicone, Breast Implants from Three Different Manufacturers

International audienc

New strategies of fibronectin grafting onto polystyrene supports

International audienc

New strategies for immobilization of vegetal adhesion protein

BIOMAT 2017 réunira chercheurs, étudiants, industriels et le monde socio-économique du 12 au 15 juin 2017 à Ambleteuse (Hauts-de-France) au sein du VVF « le Cap D’opale ». Ce colloque sera organisé par l’Association Française BIOMAT (Association pour le développement des biomatériaux, de l’ingénierie tissulaire et de la médecine régénératrice) en lien étroit avec la Fédération Régionale des Biomatériaux (SFR TS-M). Il sera associé avec des sessions communes à la 3ème journée de la Commission mixte SF2M – GFC – CEFRACOR – Titane “Matériaux pour la Santé” (du 14 au 16 Juin). Cette manifestation aura une envergure internationale. Le programme sera riche et varié, couvrant les domaines des biomatériaux et dispositifs médicaux à la médecine régénératrice, depuis la recherche jusqu’aux validations cliniques et industrielles. Sont concernées en particulier les thématiques : Matériaux avancés, Modélisation et caractérisation multi-échelles, Interface cellules-environnement, (Bio)fonctionalisations et ciblages, Suppléance et contrôle. Les considérations éthiques et d’intégration à l’humain seront également abordées. Les présentations seront illustrées par des experts de ces thématiques avec une large place réservée aux présentations orales par les plus jeunes chercheurs. Les meilleures présentations orales et affichées seront récompensées par des Prix

New strategies for immobilization of vegetal adhesion protein

BIOMAT 2017 réunira chercheurs, étudiants, industriels et le monde socio-économique du 12 au 15 juin 2017 à Ambleteuse (Hauts-de-France) au sein du VVF « le Cap D’opale ». Ce colloque sera organisé par l’Association Française BIOMAT (Association pour le développement des biomatériaux, de l’ingénierie tissulaire et de la médecine régénératrice) en lien étroit avec la Fédération Régionale des Biomatériaux (SFR TS-M). Il sera associé avec des sessions communes à la 3ème journée de la Commission mixte SF2M – GFC – CEFRACOR – Titane “Matériaux pour la Santé” (du 14 au 16 Juin). Cette manifestation aura une envergure internationale. Le programme sera riche et varié, couvrant les domaines des biomatériaux et dispositifs médicaux à la médecine régénératrice, depuis la recherche jusqu’aux validations cliniques et industrielles. Sont concernées en particulier les thématiques : Matériaux avancés, Modélisation et caractérisation multi-échelles, Interface cellules-environnement, (Bio)fonctionalisations et ciblages, Suppléance et contrôle. Les considérations éthiques et d’intégration à l’humain seront également abordées. Les présentations seront illustrées par des experts de ces thématiques avec une large place réservée aux présentations orales par les plus jeunes chercheurs. Les meilleures présentations orales et affichées seront récompensées par des Prix

Nouvelle stratégie de bio-fonctionnalisation des matériaux basée sur un greffage via les glycosylations

International audienc

Nouvelle stratégie de bio-fonctionnalisation des matériaux basée sur un greffage via les glycosylations

International audienc