Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle

© 2008 Pringle et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

Testing With Bars From Dynamic to Quasi-static

International audienceThe numerical calculation of the dynamic loading of a structure includes a great number of steps in which various fundamental or engineering problems are involved. Most of them are addressed in the present course at CISM. In this paper, we discuss the testing of materials in order to model their behaviour

Metal levels in the bones and livers of globally threatened marbled teal and white-headed duck from El Hondo, Spain

El Hondo is a key area for marbled teal and white-headed duck. We present Pb, Cu, Zn, Se, and As data for bone and liver in birds found dead between 1996 and 2001. Several metals were higher in adult white-headed ducks than in marbled teal. They were higher in female than in male white-headed ducks, and did not differ with sex in marbled teal, but did by age. Lead in liver of adults was influenced by Pb shot ingestion, which was detected in 21% of marbled teal and in 71% of white-headed duck. No marbled teal had liver levels indicative of Pb poisoning, while 86% of white-headed ducks did. Selenium, Zn, and Cu were elevated in 13%, 7%, and 39% of birds, respectively. Whilst Pb shot poses the greatest threat to these species, further work should assess exposure via plants, invertebrates, water, and sediments for other metals, and investigate possible sub-lethal effects.Peer reviewe

Lead isotopes and lead shot ingestion in the globally threatened marbled teal (Marmaronetta angustirostris) and white-headed duck (Oxyura leucocephala)

Lead isotope ratios (206Pb/207Pb and 208Pb/207Pb) and concentrations in the livers and bones of marbled teal and white-headed duck found dead or moribund were determined in order to establish the main lead source in these waterfowl species. Lead concentrations in bone (dry weight) and liver (wet weight) were found to be very high in many of the white-headed ducks (bone: geometric mean = 88.9 ppm, maximum = 419 ppm; liver: geometric mean = 16.8 ppm, maximum = 57.0 ppm). Some of the marbled teal had high lead levels in the bones but liver lead levels were all low (bone: geometric mean = 6.13 ppm, maximum = 112 ppm; liver: geometric mean = 0.581 ppm, maximum = 4.77 ppm). Ingested lead shot were found in 71% of the white-headed duck and 20% of the marbled teal. The 206Pb/207Pb ratio in livers and bones of white-headed ducks and marbled teals showed no significant differences compared to the ratios obtained from lead shot. The 206Pb/207Pb ratio in bones of marbled teal ducklings with the highest lead concentrations tended to resemble the ratios of lead shot, which supports our hypothesis that the lead was derived from the hens. We also found that the lead ratios of lead shot and lead ratios described for soils in the area overlapped, but also that the isotopic ratio 206Pb/207Pb in lead shot used in Spain has a narrow range compared with those used in North America. The principal source of lead in many of these birds was, however, most likely lead shot, as supported by the similar isotopic ratios, high lead concentrations in tissues and evidence of ingested shotPeer reviewe

Missense mutation in PFKM associated with muscle-type phosphofructokinase deficiency in the Wachtelhund dog

Hereditary muscle-type phosphofructokinase (PFK) deficiency causing intermittent hemolytic anemia and exertional myopathy due to a single nonsense mutation in PFKM has been previously described in English Springer and American Cocker Spaniels, Whippets, and mixed breed dogs. We report here on a new missense mutation associated with PFK deficiency in Wachtelhunds. Coding regions of the PFKM gene were amplified from genomic DNA and/or cDNA reverse-transcribed from RNA of EDTA blood of PFK-deficient and clinically healthy Wachtelhunds and control dogs. The amplicons were sequenced and compared to the published canine PFKM sequence. A point mutation (c.550C>T, in the coding sequence of PFKM expressed in blood) was found in all 4 affected Wachtelhunds. This missense mutation results in an amino acid substitution of arginine (Arg) to tryptophan (Trp) at position 184 of the protein expressed in blood (p.Arg184Trp). The mutation is located within an alpha-helix, and based on the SIFT analysis, this amino acid substitution is not tolerated. Amplifying the region around this mutation and digesting the PCR fragment with the restriction enzyme MspI, produces fragments that readily differentiate between PFK-deficient, carrier, and normal animals. Furthermore, we document 2 additional upstream PFKM exons expressed in canine testis but not in blood. Despite their similar phenotypic appearance and use for hunting, Wachtelhunds and English Springer Spaniels are not thought to have common ancestors. Thus, it is not surprising that different mutations are responsible for PFK deficiency in these breeds. Knowledge of the molecular basis of PFK deficiency in Wachtelhunds provides an opportunity to screen and control the spread of this deleterious trait

Hunting as a source of alien species: a European review

Hunting activities are responsible for the translocation and restocking of millions of animals throughout Europe, including the introduction of alien species. In a context of the growing use of game translocations and of increasing concern about the impact of biological invasions, our goal is to review the role of alien species introduced primarily for hunting purposes on the European scale. In particular, we explore: (1) the relative importance of game species in the context of alien species introductions; (2) the temporal evolution of the number of species introduced for hunting purposes; (3) the contribution of different taxa; (4) the pattern of introduced game species composition across countries (in terms of similarity), and (5) the underlying human demographic factors driving the diversity of introduced game species per country. According to our results, 24.3% of the mammals and 30.2% of the birds introduced into Europe during the last century were released primarily for hunting purposes, in total, 93 species (63 birds and 36 mammals), the most important taxa being Artiodactyls, Anseriformes and Galliformes. The species composition differed among countries, with a higher diversity of introduced game species in larger countries and in those with a higher human population density and proportion of hunters. This review stresses that hunting was a significant pathway for the introduction of invasive species into Europe in the last century. Since some of the game species introduced have had severe environmental impacts on many European regions, and introductions of non-native game species are still occurring, it is essential to improve regulations and increase public awareness regarding invasive game animals. This will help to preserve biodiversity and improve the sustainability of current hunting schemes in increasingly managed European ecosystems.This study received financial support from the research Project AGL2012-40128-C03-01 and EU-FEDER funds. AJC holds an FPI pre-doctoral scholarship. M. Delibes-Mateos is supported by V Plan Propio de Investigación of the University of Sevilla.Peer reviewe