A model for cofactor use during HIV-1 reverse transcription and nuclear entry.

Lentiviruses have evolved to infect and replicate in a variety of cell types in vivo whilst avoiding the powerful inhibitory activities of restriction factors or cell autonomous innate immune responses. In this review we offer our opinions on how HIV-1 uses a series of host proteins as cofactors for infection. We present a model that may explain how the capsid protein has a fundamental role in the early part of the viral lifecycle by utilising cyclophilin A (CypA), cleavage and polyadenylation specificity factor-6 (CPSF6), Nup358 and TNPO3 to orchestrate a coordinated process of DNA synthesis, capsid uncoating and integration targeting that evades innate responses and promotes integration into preferred areas of chromatin

The mass and radius of the M dwarf companion to GD 448

We present spectroscopy and photometry of GD 448, a detached white dwarf - M dwarf binary with a period of 2.47h. We find that the NaI 8200A feature is composed of narrow emission lines due to irradiation of the M dwarf by the white dwarf within broad absorption lines that are essentially unaffected by heating. Combined with an improved spectroscopic orbit and gravitational red shift measurement from spectra of the H-alpha line, we are able to derive masses for the white dwarf and M dwarf directly (0.41 +/- 0.01 solar masses and 0.096 +/- 0.004 solar masses, respectively). We use a simple model of the CaII emission lines to establish the radius of the M dwarf assuming the emission from its surface to be proportional to the incident flux per unit area from the white dwarf. The radius derived is 0.125 +/- 0.020 solar radii. The M dwarf appears to be a normal main-sequence star in terms of its mass and radius and is less than half the size of its Roche lobe. The thermal timescale of the M dwarf is much longer than the cooling age of the white dwarf so we conclude that the M dwarf was unaffected by the common-envelope phase. The anomalous width of the H-alpha emission from the M dwarf remains to be explained, but the strengh of the line may be due to X-ray heating of the M dwarf due to accretion onto the white dwarf from the M dwarf wind.Comment: 8 pages, 8 figure

NGC 602 Environment, Kinematics and Origins

The young star cluster NGC 602 and its associated HII region, N90, formed in a relatively isolated and diffuse environment in the Wing of the Small Magellanic Cloud. Its isolation from other regions of massive star formation and the relatively simple surrounding HI shell structure allows us to constrain the processes that may have led to its formation and to study conditions leading to massive star formation. We use images from Hubble Space Telescope and high resolution echelle spectrographic data from the Anglo-Australian Telescope along with 21-cm neutral hydrogen (HI) spectrum survey data and the shell catalogue derived from it to establish a likely evolutionary scenario leading to the formation of NGC 602. We identify a distinct HI cloud component that is likely the progenitor cloud of the cluster and HII region which probably formed in blister fashion from the cloud's periphery. We also find that the past interaction of HI shells can explain the current location and radial velocity of the nebula. The surrounding Interstellar Medium is diffuse and dust-poor as demonstrated by a low visual optical depth throughout the nebula and an average HI density of the progenitor cloud estimated at 1 cm^-3. These conditions suggest that the NGC 602 star formation event was produced by compression and turbulence associated with HI shell interactions. It therefore represents a single star forming event in a low gas density region.Comment: Accepted for publication in PASP. 25 pages, 10 figure

A Search for Distant Galactic Cepheids Toward l=60

We present results of a survey of a 6-square-degree region near l=60, b=0 to search for distant Milky Way Cepheids. Few MW Cepheids are known at distances >~ R_0, limiting large-scale MW disk models derived from Cepheid kinematics; this work was designed to find a sample of distant Cepheids for use in such models. The survey was conducted in the V and I bands over 8 epochs, to a limiting I~=18, with a total of ~ 5 million photometric observations of ~ 1 million stars. We present a catalog of 578 high-amplitude variables discovered in this field. Cepheid candidates were selected from this catalog on the basis of variability and color change, and observed again the following season. We confirm 10 of these candidates as Cepheids with periods from 4 to 8 days, most at distances > 3 kpc. Many of the Cepheids are heavily reddened by intervening dust, some with implied extinction A_V > 10 mag. With a future addition of infrared photometry and radial velocities, these stars alone can provide a constraint on R_0 to 8%, and in conjunction with other known Cepheids should provide good estimates of the global disk potential ellipticity.Comment: 18 pages, 4 tables, 13 figures (LaTeX / AASTeX

A photometric and spectroscopic study of NSVS 14256825: the second sdOB+dM eclipsing binary

We present an analysis of UBVR$_{\rm C}$I$_{\rm C}$JH photometry and phase-resolved optical spectroscopy of NSVS 14256825, an HW Vir type binary. The members of this class consist of a hot subdwarf and a main-sequence low-mass star in a close orbit ($P_{\rm orb} ~ 0.1$ d). Using the primary-eclipse timings, we refine the ephemeris for the system, which has an orbital period of 0.11037 d. From the spectroscopic data analysis, we derive the effective temperature, $T_1 = 40000 \pm 500$ K, the surface gravity, $\log g_1 = 5.50\pm0.05$, and the helium abundance, $n(\rm He)/n(\rm H)=0.003\pm0.001$, for the hot component. Simultaneously modelling the photometric and spectroscopic data using the Wilson-Devinney code, we obtain the geometrical and physical parameters of NSVS 14256825. Using the fitted orbital inclination and mass ratio (i = 82\fdg5\pm0\fdg3 and $q = M_2/M_1 = 0.260\pm0.012$, respectively), the components of the system have $M_1 = 0.419 \pm 0.070 M_{\odot}$, $R_1 = 0.188 \pm 0.010 R_{\odot}$, $M_2 = 0.109 \pm 0.023 M_{\odot}$, and $R_2 = 0.162 \pm 0.008 R_{\odot}$. From its spectral characteristics, the hot star is classified as an sdOB star.Comment: 8 pages, 7 figures, accepted for publication in MNRA

MxB sensitivity of HIV-1 is determined by a highly variable and dynamic capsid surface

The type one interferon induced restriction factor Myxovirus resistance B (MxB) restricts HIV-1 nuclear entry evidenced by inhibition of 2-LTR but not linear forms of viral DNA. The HIV-1 capsid is the key determinant of MxB sensitivity and cofactor binding defective HIV-1 capsid mutants P90A (defective for cyclophilin A and Nup358 recruitment) and N74D (defective for CPSF6 recruitment) have reduced dependency on nuclear transport associated cofactors, altered integration targeting preferences and are not restricted by MxB expression. This has suggested that nuclear import mechanism may determine MxB sensitivity. Here we have use genetics to separate HIV-1 nuclear import cofactor dependence from MxB sensitivity. We provide evidence that MxB sensitivity depends on HIV-1 capsid conformation, rather than cofactor recruitment. We show that depleting CPSF6 to change nuclear import pathway does not impact MxB sensitivity, but mutants that recapitulate the effect of Cyclophilin A binding on capsid conformation and dynamics strongly impact MxB sensitivity. We demonstrate that HIV-1 primary isolates have different MxB sensitivities due to cytotoxic T lymphocyte (CTL) selected differences in Gag sequence but similar cofactor dependencies. Overall our work demonstrates a complex relationship between cyclophilin dependence and MxB sensitivity likely driven by CTL escape. We propose that cyclophilin binding provides conformational flexibility to HIV-1 capsid facilitating simultaneous evasion of capsid-targeting restriction factors including TRIM5 as well as MxB

Past and present star formation in the SMC: NGC 346 and its neighborhood

In the quest of understanding how star formation occurs and propagates in the low metallicity environment of the Small Magellanic Cloud (SMC), we acquired deep F555W (~V), and F814W (~I) HST/ACS images of the young and massive star forming region NGC 346. These images and their photometric analysis provide us with a snapshot of the star formation history of the region. We find evidence for star formation extending from ~10 Gyr in the past until ~150 Myr in the field of the SMC. The youngest stellar population (~3 +/- 1 Myr) is associated with the NGC 346 cluster. It includes a rich component of low mass pre-main sequence stars mainly concentrated in a number of sub-clusters, spatially co- located with CO clumps previously detected by Rubio et al. (2000). Within our analysis uncertainties, these sub-clusters appear coeval with each other. The most massive stars appear concentrated in the central sub-clusters, indicating possible mass segregation. A number of embedded clusters are also observed. This finding, combined with the overall wealth of dust and gas, could imply that star formation is still active. An intermediate age star cluster, BS90, formed ~4.3 +/-0.1 Gyr ago, is also present in the region. Thus, this region of the SMC has supported star formation with varying levels of intensity over much of the cosmic time.Comment: 38 pages, 13 figures, 3 tables; AJ accepte

Subarcsecond Submillimeter Imaging of the Ultracompact HII Region G5.89-0.39

We present the first subarcsecond submillimeter images of the enigmatic ultracompact HII region (UCHII) G5.89-0.39. Observed with the SMA, the 875 micron continuum emission exhibits a shell-like morphology similar to longer wavelengths. By using images with comparable angular resolution at five frequencies obtained from the VLA archive and CARMA, we have removed the free-free component from the 875 micron image. We find five sources of dust emission: two compact warm objects (SMA1 and SMA2) along the periphery of the shell, and three additional regions further out. There is no dust emission inside the shell, supporting the picture of a dust-free cavity surrounded by high density gas. At subarcsecond resolution, most of the molecular gas tracers encircle the UCHII region and appear to constrain its expansion. We also find G5.89-0.39 to be almost completely lacking in organic molecular line emission. The dust cores SMA1 and SMA2 exhibit compact spatial peaks in optically-thin gas tracers (e.g. 34SO2), while SMA1 also coincides with 11.9 micron emission. In CO(3-2), we find a high-velocity north/south bipolar outflow centered on SMA1, aligned with infrared H2 knots, and responsible for much of the maser activity. We conclude that SMA1 is an embedded intermediate mass protostar with an estimated luminosity of 3000 Lsun and a circumstellar mass of ~1 Msun. Finally, we have discovered an NH3 (3,3) maser 12 arcsec northwest of the UCHII region, coincident with a 44 GHz CH3OH maser, and possibly associated with the Br gamma outflow source identified by Puga et al. (2006).Comment: 41 pages, 11 figures, published in The Astrophysical Journal (2008) Volume 680, Issue 2, pp. 1271-1288. An error in the registration of the marker positions in Figure 11 has been corrected in this versio

Large Numbers in Computing and Mathematics

We present an overview of large numbers within mathematics and computing. Particular emphasis is put on the problem of large number notation in the mathematical attempt to get closer to infinity