Mechanical properties and cytotoxic evaluation of the Ti-3Nb-13Zr alloy

Ti-13Nb-13Zr is a new titanium alloy that was originally developed for medical implant applications. This alloy combines a low elastic modulus, high strength, excellent hot and cold workability, and superior corrosion resistance. Research on this alloy has shown that the mechanical properties can be controlled over a significant range through hot working, heat treatment and cold-working. The present study describes the mechanical properties and cytotoxic evaluation of the Ti-13Nb-13Zr alloy, which was produced by furnace arc melting in argon atmosphere. The elemental constituents were unalloyed Ti, Nb and Zr sheets. The obtained ingots, which initial diameter were about 15 mm, have undergone sequences of cold-working and heat treatments in order to achieve a final diameter of 6 mm. The tensile strength of Ti-13Nb-13Zr achieved 1270 MPa (cold-worked – 60% reduction in area) and 860 MPa after heat treatment (60% reduction in area + 1000 o C/1h + water quenched). The elastic module were 52 GPa and 60 GPa respectively. Furthermore, the toxic effect of this alloy on cells was evaluated by a cytotoxicity test, a quantitative method of colony suppression assay using Chinese Hamster Ovary (CHO) cultured cells in contact with diluted extracts of the biomaterials. The results showed that Ti-13Nb-13Zr alloy obtained by furnace arc melting isn’t cytotoxic.Peer Reviewe

Mechanical properties and cytotoxic evaluation of the Ti-3Nb-13Zr alloy

Ti-13Nb-13Zr is a new titanium alloy that was originally developed for medical implant applications. This alloy combines a low elastic modulus, high strength, excellent hot and cold workability, and superior corrosion resistance. Research on this alloy has shown that the mechanical properties can be controlled over a significant range through hot working, heat treatment and cold-working. The present study describes the mechanical properties and cytotoxic evaluation of the Ti-13Nb-13Zr alloy, which was produced by furnace arc melting in argon atmosphere. The elemental constituents were unalloyed Ti, Nb and Zr sheets. The obtained ingots, which initial diameter were about 15 mm, have undergone sequences of cold-working and heat treatments in order to achieve a final diameter of 6 mm. The tensile strength of Ti-13Nb-13Zr achieved 1270 MPa (cold-worked – 60% reduction in area) and 860 MPa after heat treatment (60% reduction in area + 1000 o C/1h + water quenched). The elastic module were 52 GPa and 60 GPa respectively. Furthermore, the toxic effect of this alloy on cells was evaluated by a cytotoxicity test, a quantitative method of colony suppression assay using Chinese Hamster Ovary (CHO) cultured cells in contact with diluted extracts of the biomaterials. The results showed that Ti-13Nb-13Zr alloy obtained by furnace arc melting isn’t cytotoxic.Peer Reviewe

Dimensional Change Assessment Of Lyophilized Bovine Pericardium

[No abstract available]21141Maizato, M.J.S., Higa, O.Z., Mathor, M.B., Camillo, M.A.P., Spencer, P.J., Pitombo, R.N., Zavaglia, C.A.C., Leirner, A.A., Glutaraldehyde-treated bovine pericardium: Effects of lyophilization on citotoxicity and residual aldehydes (2003) Artificial Organs, 27, p. 69

α-tricalcium Phosphate Cement: "in Vitro" Cytotoxicity

Calcium phosphate-based bioceramics have revolutionized orthopedic and dental repair of damaged parts of the bone system. Among these materials, calcium phosphate-based cements, with hydraulic setting, stand out due to their biocompatibility and in situ hardening, which allow easy manipulation and adaptation to the shape and dimensions of bone defects. An investigation was made of the in vitro cytotoxic effect of calcium phosphate cement based on α-tricalcium phosphate, immersed for different lengths of time in simulated body fluid (SBF), based on the ISO-10993 "Biological Evaluation of Medical Devices" standard. The culture medium was Chinese hamster ovary (CHO) cells in contact with diluted cement extracts. The results revealed that the calcium phosphate cement used was cytotoxic and that the material's cytotoxicity decreased the longer the cement was immersed in SBF. © 2002 Elsevier Science Ltd. All rights reserved.23920352042Gruninger, S.E., Siew, C., Chow, L.C., O'Young, A., Ts'ao, N.K., Brown, W.E., Evaluation of the biocompatibility of a new calcium-phosphate setting cement (1984) J Dent Res, pp. 63-200Yu, D., Wong, J., Matsuda, Y., Fox, J.L., Higuchi, W.I., Otsuka, M., Self-setting hydroxyapatite cement: A novel skeletal drug delivery system for antibiotics (1992) J Pharm Sci, 81 (6), pp. 529-531Driessens, F.C.M., Fernández, E., Ginebra, M.P., Boltong, M.G., Planell, J.A., Calcium phosphates and ceramic bone cements vs. acrylic cements (1997) Anal Quim Int Ed, 93, pp. S38-S43Monma, H., Goto, M., Kohmura, T., Effect of additives on hydration and hardness of tricalcium phosphate (1984) Gypsum lime, 188, pp. 11-16Ginebra, M.P., Fernández, E., Driessens, F.C.M., Planell, J.A., The effect of Na2HPO4 addition on the setting reaction kinetics of an α-TCP cement (1998) Biomaterials, 11, pp. 243-246Jenkins, R., Vries, J.L., (1971) An introduction to X-ray powder diffractometry, , Eindhoven, Holanda: N.V. Philips Gloeilampenfabrieken(1992) International standard: Biological evaluation of medical devices - Part 5: tests for cytotoxicity: in vitro methods, , ISO 10993-5Nakamura, A., Ikarashi, Y., Tshuchiya, T., Kaniwa, M., Radiation vulcanized natural rubber latex is not cytotoxic (1989) Proceedings of the International Symposium on Radiation Vulcanization of Natural Rubber Latex, pp. 79-87. , Japan Atomic Research Institute JAERI-M 89-228, Takasaki, JapanDriessens, F.C.M., Boltong, M.G., Bermudez, O., Planell, J., Efective formulations for the preparation of calcium phosphate bone cements (1994) J Mater Sci Mater Med, 5, pp. 164-170Fernández, E., Ginebra, M.P., Bermudez, O., Boltong, M.G., Driessens, F.C.M., Dimensional and thermal behaviour of calcium phosphate cements during setting compared to PMMA bone cements (1995) J Mater Sci Lett, 14, pp. 4-5Carrodéguas, R.G., Rigo, E.C., Oliveira, L.C., Santos, L.A., Boschi, A.O., Recubrimiento de hidroxiapatita sobre ceramica de titanato de bario BIOMAT'97 - Congresso Internacional de Biomateriales, , 4 de Maio/1997, Universidad de La Habana, CubaLi, P., Ohtswki, C., Kokubo, T., Apatite formation induced by silica gel in a simulated body fluid (1992) J Am Ceram Soc, 75, pp. 2094-2097Fresa, R., Constantini, A., Buri, A., Apatite formation on (2-x)CaO·x/3M2O3·2SiO2 glasses (M=La, Y0<x<0.6) in a simulated body fluid (1995) Biomaterials, 16, pp. 849-854Oliveira, J.M., Correia, R., Fernandes, M.H., Surface modifications of a glass and a glass-ceramic of the MgO-3CaO·P2O5-SiO2 system in a simulated body fluid (1995) Biomaterials, 16, pp. 849-854Santos, L.A., Oliveira, L.C., Rigo, E.C.S., Carrodéguas, R.G., Boschi, A.O., Arruda, A.C.F., Influence of polymeric additives on the mechanical properties of α-tricalcium phosphate cement (1999) Bone, 25 (2), pp. 99S-102SSantos, L.A., Oliveira, L.C., Rigo, E.C.S., Carrodéguas, R.G., Boschi, A.O., Arruda, A.C.F., Fiber reinforced calcium phosphate cement (2000) Artif Organs, 24 (3), pp. 212-216Carrodéguas, R.G., Santos, L.A., Rigo, E.C.S., Mondéjar, S.P., Arruda, A.C.F., Boschi, A.O., Improvement of mechanical strength of calcium phosphate cement by dual-setting principle J Mater Sci Mater Med, , submitted for publicationBermúdez, O., Boltong, M.G., Driessens, F.C.M., Planell, J.A., Development of an octacalcium phosphate cement (1994) J Mater Sci Mater Med, 5, pp. 144-146Tampieri, A., Celotti, G., Szontagh, F., Landi, E., Sintering and characterization of HA and TCP bioceramics with control of their strength and phase purity (1997) J Mater Sci Mater Med, 8, pp. 29-37Famery, R., Richard, N., Boch, P., Preparation of α- and β-tricalcium phosphate ceramics with and without magnesium addition (1994) Ceram Int, 20, pp. 327-336Fernández, E., Ginebra, M.P., Boltong, M.G., Verbeeck, R.M.H., Planell, J.A., Kinetic study of the setting reaction of a calcium phosphate bone cement (1996) J Biomed Mater Res, 32, pp. 367-374Ginebra, M.P., Fernández, E., De Maeyer, E.A.P., Verbeeck, R.M.H., Boltong, M.G., Ginebra, J., Driessens, F.C.M., Planell, J.A., Setting reaction and hardening of an apatitic calcium phosphate cement (1997) J Dent Res, 76 (4), pp. 905-912Driessens, F.C.M., Boltong, M.G., Bermúdez, O., Planell, J.A., Ginebra, M.P., Fernández, E., Effective formulations for the preparation of calcium phosphate bone cements (1994) J Mater Sci Mater Med, 5, pp. 164-170Bermúdez, O., Boltong, M.G., Driessens, F.C.M., Planell, J.A., Development of some calcium phosphate cements from combinations of α-TCP, MCPM and CaO (1994) J Mater Sci Mater Med, 5, pp. 160-163Fernández, E., Gil, J.F., Ginebra, M.P., Driessens, F.C.M., Planell, J.A., Production and characterization of new calcium phosphate bone cements in the CaHPO4-α-Ca3(PO4)2 system: pH, workability and setting times (1999) J Mater Sci Mater Med, 10, pp. 223-230Chow, L.C., Development of self-setting calcium phosphate cements (1991) J Ceram Soc Jpn (The Centennial Memorial Issue), 99 (10), pp. 954-964Ishikawa, K., Miyamoto, Y., Suzuki, K., Nagayama, M., Mechanism of inflamatory response to calcium phosphate cement (1998) J Dent Res, 1446, p. 812Miyamoto, Y., Ishikawa, K., Takechi, M., Toh, T., Yuasa, T., Nagayama, M., Suzuki, K., Histological and composicional evaluations of three types of calcium phosphate cements when implanted in subcutaneous tissue immediately after mixing (1999) J Biomed Mater Res, 48, pp. 36-4

Lyophilized Bovine Pericardium Coated With Silk Fibroin Solution As An Alternative To Prevent Calcification Of Cardiovascular Bioprosthesis: Preliminary Calcification Results

[No abstract available]1509Pettenazzo, E., Dynamic in vitro calcification of bioprosthetic porcine valves: Evidence of apatite crystallization (2001) The Journal of Thoracic and Cardiovascular Surgery, 121 (3), pp. 500-509Aimoli, C.G., Lyophilized bovine pericardium treated with a phenethylaminediepoxide as an alternative to preventing calcification of cardiovascular bioprosthesis: Preliminary calcification results (2007) Artificial Organs, 31 (4), pp. 278-28

Adherence And Growth Of Endothelial Cells On Silk Fibroin Dense Membranes

[No abstract available]2839Altman, G.H., Diaz, F., Jakuba, C., Calabro, T., Horan, R.L., Chen, J., Lu, H., Kaplan, D.L., Silkbased biomaterials (2003) Biomaterials, 24, p. 401Servoli, E., Maniglio, D., Motta, A., Predazzer, R., Migliaresi, C., Surface properties of silk fibroin films and their interaction with fibroblasts (2005) Macromol Biosci., 5, p. 1175Gupta, M.K., Khokhar, S.K., Phillips, D.M., Sowards, L.A., Drummy, L.F., Kadakia, M.P., Naik, R.R., Patterned silk films cast from ionic liquid solubilized fibroin as scaffolds for cell growth (2007) Langmuir, 23, p. 1315Fuchs, S., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Outgrowth endothelial cells isolated and expanded from human peripheral blood progenitor cells as a potential source of autologous cells for endothelialization of silk fibroin biomaterials (2006) Biomaterials, 27, p. 5399Feugier, P., Black, R.A., Hunt, J.A., How, T.V., Attachment, morphology and adherence of human endothelial cells to vascular prosthesis materials under the action of shear stress (2005) Biomaterials, 26, p. 145