Multiconfiguration Loads Analysis for Missions with an Uncertain Rideshare Manifest

Rideshare or “multi-manifest” missions – where several smaller “rideshare” spacecraft are launched together, usually with a larger “forward” spacecraft – are becoming increasingly common. In many cases, the properties or configuration of the rideshare spacecraft are not well-defined during initial launch manifesting and may not be finalized until a few months before launch. In this paper, a multiconfiguration loads analysis (MLA) process is presented that can enable flexibility in the mission manifesting process by allowing for uncertainty in the final rideshare configuration, including late manifest changes or swaps, without requiring additional loads analyses to those specified in the Load Cycle Process. By applying the MLA process, a set of adequately conservative loads can be generated for the forward spacecraft, launch vehicle, and potential rideshare spacecraft that account for uncertainty in the rideshare manifest and minimize the potential for issues late in the process. The MLA process will also define a mission-specific dynamic properties envelope that would allow rideshare spacecraft that “fit” within the envelope to be swapped. If all parts of the system are verified to match the models used in analysis and designed to survive the bounding loads, the launch manifest can be changed after analyses are completed, much closer to launch, without incurring increased risk

LGR1 signalling is required in the development of the fruit fly, Drosophila melanogaster

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An evolutionary comparison of leucine-rich repeat containing G protein-coupled receptors reveals a novel LGR subtype

Leucine-rich repeat containing G protein-coupled receptors or LGRs are receptors with important functions in development and reproduction. Belonging to this evolutionarily conserved group of receptors are the well-studied glycoprotein hormone receptors and relaxin receptors in mammals, as well as the bursicon receptor, which triggers cuticle hardening and tanning in freshly enclosed insects. In this study, the numerous LGR sequences in different animal phyla are analyzed and compared. Based on these data a phylogenetic tree was generated. This information sheds new light on structural and evolutionary aspects regarding this receptor group. Apart from vertebrates and insects, LGRs are also present in early chordates (Urochordata, Cephalochordata and Hyperoartia) and other arthropods (Arachnida and Branchiopoda) as well as in Mollusca, Echinodermata, Hemichordata, Nematoda, and even in ancient animal life forms, such as Cnidaria and Placozoa. Three distinct types of LGR exist, distinguishable by their number of leucine-rich repeats (LRRs), their type-specific hinge region and the presence or absence of an LDLa motif. Type C LGRs containing only one LDLa (C1 subtype) appear to be present in nearly all animal phyla. We here describe a second subtype, C2, containing multiple LDLa motifs, which was discovered in echinoderms, mollusks and in one insect species (Pediculus humanis corporis). In addition, eight putative LGRs can be predicted from the genome data of the placozoan species Trichoplax adhaerens. They may represent an ancient form of the LGRs, however, more genomic data will be required to confirm this hypothesis.status: publishe

Tachykinin-related peptides and their receptors in invertebrates: a current view

Members of the tachykinin peptide family have been well conserved during evolution and are mainly expressed in the central nervous system and in the intestine of both vertebrates and invertebrates. In these animals, they act as multifunctional messengers that exert their biological effects by specifically interacting with a subfamily of structurally related G protein-coupled receptors. Despite the identification of multiple tachykinin-related peptides (TKRPs) in species belonging to the insects, crustaceans, mollusks and echiuroid worms, only five invertebrate receptors harboring profound sequence similarities to mammalian receptors for tachykinins have been functionally characterized to date. Three of these have been cloned from dipteran insect species, i.e. NKD (neurokinin receptor from Drosophila), DTKR (Drosophila tachykinin receptor) and STKR (tachykinin-related peptide receptor from the stable fly, Stomoxys calcitrans). In addition, two receptors from non-insect species, present in echiuroid worms and mollusks, respectively have been identified as well. In this brief review, we will survey some recent findings and insights into the signaling properties of invertebrate tachykinin-related peptides via their respective receptors. In this context, we will also point out the necessity to take into account differences in signaling mechanisms induced by distinct TKRP isoforms in insects.status: publishe

Neuroparsins, a family of conserved arthropod neuropeptides

Different neuroparsin variants were initially identified as anti-gonadotropic peptides from the pars intercerebralis-corpora cardiaca complex of the migratory locust, Locusta migratoria, and further studies revealed the pleiotropic activities of these peptides. Subsequently, additional neuroparsin-like peptides were discovered from other arthropod species. Studies in mosquitoes and locusts suggest that members of this conserved peptide family are involved in the regulation of insect reproduction and can even serve as molecular markers of the fascinating biological process of locust phase transition. Sequence analysis and multiple alignments revealed pronounced sequence similarities between arthropod neuroparsins and the N-terminal, growth factor binding region of vertebrate and mollusc insulin-like growth factor binding proteins (IGFBP). This observation led to the hypothesis that neuroparsins might interact with endogenous insulin-related peptides. The present paper gives an overview of several neuroparsin family members that have hitherto been described in insects, as well as of a number of newly identified neuroparsin precursors from other species.status: publishe

Neuropeptide Receptors as Possible Targets for Development of Insect Pest Control Agents

Vaious insect species have a severe impact on human welfare and environment and thus force us to continuously develop novel agents for pest control. Neuropeptides constitute a very versatile class of bioactive messenger molecules that initiate and/or regulate a wide array of vital biological processes in insects by acting on their respective receptors in the plasmamembrane of target cells. These receptors belong to two distinct categories of signal transducing proteins, i.e., heptahelical or G protein-coupled receptors (7TM, GPCR) and single transmembrane containing receptors. An increasing amount ofevidence indicates that insect neuropeptide-receptor couples play crucial roles in processes as diverse as development, metabolism, ecdysis and reproduction. As such, they gain growing interest as promising candidate targets for the development of a new generation of species- and receptor-specific insect control agents that may generate fewer side effects. In this chapter, we will present some examples of insect neuropeptide receptors and aim to demonstrate their fundamental importance in insect biology.Advances in Experimental Medicine and Biologystatus: publishe

Evolutionary conservation of bursicon in the animal kingdom.

Bursicon bioactivity is essential for tanning of the exoskeleton and for wing spreading behavior that occur in newly emerged adult insects. Previously, we demonstrated that in the fruit fly, Drosophila melanogaster, bursicon exists as a heterodimeric cystine knot protein that activates the leucine-rich repeats containing G protein-coupled receptor 2 (DLGR2). By performing similarity based in silico searches in genomic and complementary DNA databases, we identified bursicon homologous sequences in several protostomian as well as deuterostomian invertebrates. In the genome of the honeybee, Apis mellifera, the coding regions for bursicon cystine knot subunits are organized in a genomic locus of approximately 4 kilobase pairs. Reverse transcription PCR analysis indicates that this region likely codes for two distinct bursicon cystine knot subunits. Our results illustrate the remarkable conservation of bursicon in invertebrate species and provide an avenue for functional analyses of this hormone in a wide range of animal species.Comparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe

Myoinhibiting peptides are the ancestral ligands of the promiscuous Drosophila sex peptide receptor

Male insects change behaviors of female partners by co-transferring accessory gland proteins (Acps) like sex peptide (SP), with their sperm. The Drosophila sex peptide receptor (SPR) is a G protein-coupled receptor expressed in the female's nervous system and genital tract. While most Acps show a fast rate of evolution, SPRs are highly conserved in insects. We report activation of SPRs by evolutionary conserved myoinhibiting peptides (MIPs). Structural determinants in SP and MIPs responsible for this dual receptor activation are characterized. Drosophila SPR is also expressed in embryonic and larval stages and in the adult male nervous system, whereas SP expression is restricted to the male reproductive system. MIP transcripts occur in male and female central nervous system, possibly acting as endogenous SPR ligands. Evolutionary consequences of the promiscuous nature of SPRs are discussed. MIPs likely function as ancestral ligands of SPRs and could place evolutionary constraints on the MIP/SPR class.status: publishe

Comparative genomics of leucine-rich repeats containing G protein-coupled receptors and their ligands.

Leucine-rich repeats containing G protein-coupled receptors (LGRs) constitute a unique cluster of transmembrane proteins sharing a large leucine-rich extracellular domain for hormone binding. In mammals, LGRs steer important developmental, metabolic and reproductive processes as receptors for glycoprotein hormones and insulin/relaxin-related proteins. In insects, a receptor structurally related to human LGRs mediates the activity of the neurohormone bursicon thereby regulating wing expansion behaviour and remodelling of the newly synthesized exoskeleton. In the past decade, novel insights into the molecular evolution of LGR encoding genes accumulated rapidly due to comparative genome analyses indicating that the endocrine LGR signalling system likely emerged before the radiation of metazoan phyla and expanded throughout evolution. Here, we present a short survey on the evolution of LGRs and the hormones they interact with.Comparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tReviewSCOPUS: sh.jinfo:eu-repo/semantics/publishe