A Jump Ornstein-Uhlenbeck Bridge Based on Energy-optimal Control and Its Self-exciting Extension

We study a version of the Ornstein-Uhlenbeck bridge driven by a spectrally-positive subordinator. Our formulation is based on a Linear-Quadratic control subject to a singular terminal condition. The Ornstein-Uhlenbeck bridge, we develop, is written as a limit of the obtained optimally controlled processes, and is shown to admit an explicit expression. Its extension with self-excitement is also considered. The terminal condition is confirmed to be satisfied by the obtained process both analytically and numerically. The methods are also applied to a streamflow regulation problem using a real-life dataset.Comment: This is a revised versio

Large-scale stem cell production system by newly designed bioreactor

Large scale cell production system is required in drug discovery and cell based therapy. However there are technically challenges in maintaining high quality and low cost for practical biomanufacturing industry. 3D suspension culture is one of attractive process, especially static culture is ultimate free from shear force. Utilizing static 3D suspension culture, automated closed system is newly designed and demonstrated expansion culture over several subcultures. Cell growth curves of this system and conventional manual method have good accordance. The system is also compatible with good scalability for scale up and scale out use. Please click Additional Files below to see the full abstract

Effect of Primordial Magnetic Field on Seeds for Large Scale Structure

Magnetic field plays a very important role in many astronomical phenomena at various scales of the universe. It is no exception in the early universe. Since the energy density, pressure, and tension of the primordial magnetic field affect gravitational collapses of plasma, the formation of seeds for large scale structures should be influenced by them. Here we numerically investigate the effects of stochastic primordial magnetic field on the seeds of large scale structures in the universe in detail. We found that the amplitude ratio between the density spectra with and without PMF ($|P(k)/P_0(k)|$ at $k>0.2$ Mpc$^{-1}$) lies between 75% and 130% at present for the range of PMF strengths 0.5 nG $< B_\lambda < 1.0$ nG, depending on the spectral index of PMF and the correlation between the matter density and the PMF distributions.Comment: 20 pages, 5 figures, submitted to PRD 23 Jan 2006, Revised 02 Oct 2006, accepted for publication in PR

Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies

BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. RESULTS: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. CONCLUSIONS: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies

Applicability of radiocolloids, blue dyes and fluorescent indocyanine green to sentinel node biopsy in melanoma

Patients with primary cutaneous melanoma underwent sentinel node (SN) mapping and biopsy at 25 facilities in Japan by the combination of radiocolloid with gamma probe and dye. Technetium-99m (99mTc)-tin colloid, 99mTc-phytate, 2% patent blue violet (PBV) and 0.4% indigo carmine were used as tracers. In some hospitals, 0.5% fluorescent indocyanine green, which allows visualization of the SN with an infrared camera, was concomitantly used and examined. A total of 673 patients were enrolled, and 562 cases were eligible. The detection rates of SN were 95.5% (147/154) with the combination of tin colloid and PBV, 98.9% (368/372) with the combination of phytate and PBV, and 97.2% (35/36) with the combination of tin colloid or phytate and indigo carmine. SN was not detected in 12 cases by the combination method, and the primary tumor was in the head and neck in six of those 12 cases. In eight of 526 cases (1.5%), SN was detected by PBV but not by radiocolloid. There were 13 cases (2.5%) in which SN was detected by radiocolloid but not by PBV. In 18 of 36 cases (50%), SN was detected by radiocolloid but not by indigo carmine. Concomitantly used fluorescent indocyanine green detected SN in all of 67 cases. Interference with transcutaneous oximetry by PVB was observed in some cases, although it caused no clinical trouble. Allergic reactions were not reported with any of the tracers. 99mTc-tin colloid, 99mTc-phytate, PBV and indocyanine green are useful tracers for SN mapping.ArticleJOURNAL OF DERMATOLOGY. 39(4):336-338 (2012)journal articl

Risk factors for CAR-T cell manufacturing failure among DLBCL patients: A nationwide survey in Japan

CAR-T細胞製造を成功させるためのレシピ --アフェレーシス前の下ごしらえでの工夫--. 京都大学プレスリリース. 2023-04-27.For successful chimeric antigen receptor T (CAR-T) cell therapy, CAR-T cells must be manufactured without failure caused by suboptimal expansion. In order to determine risk factors for CAR-T cell manufacturing failure, we performed a nationwide cohort study in Japan and analysed patients with diffuse large B-cell lymphoma (DLBCL) who underwent tisagenlecleucel production. We compared clinical factors between 30 cases that failed (7.4%) with those that succeeded (n = 378). Among the failures, the proportion of patients previously treated with bendamustine (43.3% vs. 14.8%; p < 0.001) was significantly higher, and their platelet counts (12.0 vs. 17.0 × 10⁴/μL; p = 0.01) and CD4/CD8 T-cell ratio (0.30 vs. 0.56; p < 0.01) in peripheral blood at apheresis were significantly lower than in the successful group. Multivariate analysis revealed that repeated bendamustine use with short washout periods prior to apheresis (odds ratio [OR], 5.52; p = 0.013 for ≥6 cycles with washout period of 3–24 months; OR, 57.09; p = 0.005 for ≥3 cycles with washout period of <3 months), low platelet counts (OR, 0.495 per 105/μL; p = 0.022) or low CD4/CD8 ratios (<one third) (OR, 3.249; p = 0.011) in peripheral blood at apheresis increased the risk of manufacturing failure. Manufacturing failure remains an obstacle to CAR-T cell therapy for DLBCL patients. Avoiding risk factors, such as repeated bendamustine administration without sufficient washout, and risk-adapted strategies may help to optimize CAR-T cell therapy for DLBCL patients

ダイジョ DIOSCOREA ALATA L. ノ ソシキバイヨウ ニ ヨル マイクロチューバケイセイ

ダイジョ(Dioscorea alata L.)の地方品種`Nani Niyaka\u27を無菌培養した植物の節部切片培養を行い,マイクロチューバ(MT)形成に及ぼす植物成長調節物質,培地の種類,培地のショ糖濃度,ならびに照明時間の効果について検討した。その結果,NAA, ABA, KINおよびPDJなど4種類の植物成長調節物質は,MT形成に顕著な効果を示さなかった。特にNAAとPDJはMT形成に抑制する傾向が見られた。3種類の基本培地,4水準の照明時間,5水準のショ糖濃度について実験を行った結果,MS培地に10%程度のショ糖を添加し,16時間照明で培養するのがMT形成に最も効果的であることが分かった。ダイジョのMT形成には,培養植物体内の炭水化物が必要と考えられる。培地の窒素の種類と濃度がMT形成に影響し,窒素成分に富む培地でMT形成が促進された。これらの結果から,植物体内の炭水化物と窒素の絶対量がMT形成の促進要因となっていると考えられる。This study aims at finding factors affecting microtuberization of greater yam (Dioscorea alata L.). None of four plant growth regulators, NAA, ABA, kinetin and PDJ, were promotive to form microtubers. The MS medium adding 10% sucrose under 16 hours illumination remarkably promoted to form microtubers. But NAA and PDJ tended to inhibit tuberization. The three-way arrangement experiments with three different culture media, five levels of sucrose concentration, and four levels of illumination time were conducted and disclosed that tuberization was the most active on the MS medium with the addition of 10% sucrose under 16 hours period of illumination a day. The essential to microtuberization of greater yam is considered to be the concentration of carbohydrate in cultured plants. Sources and concentrations of nitrogen in the culture media affected the formation of microtubers. Nitrogen-rich media promoted microtuberization

Complications Associated With Spine Surgery in Patients Aged 80 Years or Older: Japan Association of Spine Surgeons with Ambition (JASA) Multicenter Study

Study Design:Retrospective study of registry data.Objectives:Aging of society and recent advances in surgical techniques and general anesthesia have increased the demand for spinal surgery in elderly patients. Many complications have been described in elderly patients, but a multicenter study of perioperative complications in spinal surgery in patients aged 80 years or older has not been reported. Therefore, the goal of the study was to analyze complications associated with spine surgery in patients aged 80 years or older with cervical, thoracic, or lumbar lesions.Methods:A multicenter study was performed in patients aged 80 years or older who underwent 262 spinal surgeries at 35 facilities. The frequency and severity of complications were examined for perioperative complications, including intraoperative and postoperative complications, and for major postoperative complications that were potentially life threatening, required reoperation in the perioperative period, or left a permanent injury.Results:Perioperative complications occurred in 75 of the 262 surgeries (29%) and 33 were major complications (13%). In multivariate logistic regression, age over 85 years (hazard ratio [HR] = 1.007, P = 0.025) and estimated blood loss ≥500 g (HR = 3.076, P = .004) were significantly associated with perioperative complications, and an operative time ≥180 min (HR = 2.78, P = .007) was significantly associated with major complications.Conclusions:Elderly patients aged 80 years or older with comorbidities are at higher risk for complications. Increased surgical invasion, and particularly a long operative time, can cause serious complications that may be life threatening. Therefore, careful decisions are required with regard to the surgical indication and procedure in elderly patients

Risk Factors for Delirium After Spine Surgery in Extremely Elderly Patients Aged 80 Years or Older and Review of the Literature: Japan Association of Spine Surgeons with Ambition Multicenter Study

Study Design:Retrospective database analysis.Objective:Spine surgeries in elderly patients have increased in recent years due to aging of society and recent advances in surgical techniques, and postoperative complications have become more of a concern. Postoperative delirium is a common complication in elderly patients that impairs recovery and increases morbidity and mortality. The objective of the study was to analyze postoperative delirium associated with spine surgery in patients aged 80 years or older with cervical, thoracic, and lumbar lesions.Methods:A retrospective multicenter study was performed in 262 patients 80 years of age or older who underwent spine surgeries at 35 facilities. Postoperative complications, incidence of postoperative delirium, and hazard ratios of patient-specific and surgical risk factors were examined.Results:Postoperative complications occurred in 59 of the 262 spine surgeries (23%). Postoperative delirium was the most frequent complication, occurring in 15 of 262 patients (5.7%), and was significantly associated with hypertension, cerebrovascular disease, cervical lesion surgery, and greater estimated blood loss (P < .05). In multivariate logistic regression using perioperative factors, cervical lesion surgery (odds ratio = 4.27, P < .05) and estimated blood loss ≥300 mL (odds ratio = 4.52, P < .05) were significantly associated with postoperative delirium.Conclusions:Cervical lesion surgery and greater blood loss were perioperative risk factors for delirium in extremely elderly patients after spine surgery. Hypertension and cerebrovascular disease were significant risk factors for postoperative delirium, and careful management is required for patients with such risk factors