PENGARUH MADU TERHADAP GAMBARAN MIKROSKOPIS HEPAR PADA TIKUS WISTAR JANTAN YANG DIINDUKSI MONOSODIUM GLUTAMAT

Background: Monosodium glutamate (MSG) has been consumed widely as flavor enhancer. The effect of MSG is reported causing Chinese Restaurant Sindrome, hyperlipidemia, hyperglicemia, and oxydative stress. The toxic effect of liver is reported increasing lipid peroxydation, hepatocytes damage, degeneration and necrosis of liver cells. Honey has potential benefit in decreasing lipid peroxydation in liver as exogenous antioxydant. Honey has a hepatoprotective effect and is expected to repair liver damage caused by MSG. Objective: To prove the effect of multilevel doses of honey administration in liver microscopics appearance on Wistar rats induced by MSG. Method: Experimental laboratory research with a Post Test-Only Control Group Design. Sample consists of 18 male Wistar rats divided by simple random sampling into 3 groups. Group P1, P2 and P3 were given MSG orally with dose 6mg/g/day. After sixty minutes, P2 and P3 were treated orally with honey at a dose of 2g/200g/day (P2) and 4g/200g/day (P3). After 30 days intervention, all samples were terminated, livers were taken for microscopic. Analysed by Kruskal- Wallis test and Mann Whitney test. Result: Histopathologycal examination showed a dominant necrosis 2nd zone in liver induced by MSG and mild damage (parenchym degeneration) in liver microscopic treated by graded doses honey. Kruskal-Wallis test result obtained significant differences between P1,P2, and P3 (p=0.000). Mann Whitney test result obtained significant differences for P1-P2 (p=0.000), P1-P3 (p=0.000) but obtained unsignificant differences P2-P3 (p=0,277). Conclusion: The multilevel doses of honey administration gives a significant difference for liver microscopic appearance on Wistar rats which is induced by MSG. Keywords: Monosodium glutamate, honey, antioxidant, liver microscopic appearance