19,133 research outputs found
Intestinal epithelial responses to Salmonella enterica serovar Enteritidis: Effects on intestinal permeability and ion transport
Salmonella infection of chickens that leads to potential human foodborne salmonellosis continues to be a major concern. Chickens serve as carriers but, in contrast to humans, rarely show any clinical signs including diarrhea. The present investigations aimed to elucidate whether the absence of diarrhea during acute Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) infection may be linked to specific changes in the electrophysiological properties of the chicken gut. Immediately after slaughter, intestinal pieces of the mid-jejunum and cecum of either commercial broiler or specific pathogen-free (SPF) chickens were mounted in Ussing chambers in 2 separate experimental series. Living Salmonella Enteritidis (3 Ă— 109) or Salmonella Enteritidis endotoxin (20 mg/L), or both, were added to the mucosal side for 1 h. In both experimental series, the Salmonella infection decreased the trans-epithelial ion conductance Gt (P < 0.05). In the jejunum of SPF chickens, there was also a marked decrease in net charge transfer across the epithelium, evidenced by decreased short-circuit current (Isc, P < 0.05). Interestingly, the mucosal application of Salmonella endotoxin to the epithelial preparations from jejunum and cecum of SPF chicken had an effect similar to living bacteria. However, the endotoxin had no additional effect on the intestinal function in the presence of bacteria. The decreasing effect of Salmonella and or its endotoxin on Gt could be partly reversed by serosal addition of histamine. To our knowledge, this is the first study to address the functional response of native intestinal epithelium of chicken to an in vitro Salmonella infection. For the first time, it can be reported that intestinal ion permeability of chicken decreases acutely by the presence of Salmonella. This type of response could counteract ion and fluid secretion and may thus, at least in part, explain why chickens do not develop overt diarrhea after Salmonella infection
Campylobacter jejuni colonization promotes the translocation of Escherichia coli to extra-intestinal organs and disturbs the short-chain fatty acids profiles in the chicken gut
For a long time Campylobacter was only considered as a commensal microorganism in avian hosts restricted to the ceca, without any pathogenic features. The precise reasons for the symptomless chicken carriers are still unknown, but investigations of the gastrointestinal ecology of broiler chickens may improve our understanding of the microbial interactions with the host. Therefore, the current studies were conducted to investigate the effects of Campylobacter jejuni colonization on Escherichia coli translocation and on the metabolic end products (short-chain fatty acids, SCFAs). Following oral infection of 14 day old broiler chickens with 1 Ă— 108 CFU of Campylobacter jejuni NCTC 12744 in two independent animal trials, it was found that C. jejuni heavily colonized the intestine and disseminate to extra-intestinal organs. Moreover, in both animal trials, the findings revealed that C. jejuni promoted the translocation of E. coli with a higher number encountered in the spleen and liver at 14 days post infection (dpi). In addition, Campylobacter affected the microbial fermentation in the gastrointestinal tract of broilers by reducing the amount of propionate, isovalerate, and isobutyrate in the cecal digesta of the infected birds at 2 dpi and, at 7 and 14 dpi, butyrate, isobutyrate, and isovalerate were also decreased. However, in the jejunum, the C. jejuni infection lowered only butyrate concentrations at 14 dpi. These data indicated that C. jejuni may utilize SCFAs as carbon sources to promote its colonization in the chicken gut, suggesting that Campylobacter cannot only alter gut colonization dynamics but might also influence physiological processes due to altered microbial metabolite profiles.
Finally, the results demonstrated that C. jejuni can cross the intestinal epithelial barrier and facilitates the translocation of Campylobacter itself as well as of other enteric microorganisms such as E. coli to extra-intestinal organs of infected birds. Altogether, our findings suggest that the Campylobacter carrier state in chicken is characterised by multiple changes in the intestinal barrier function, which supports multiplication and survival within the host
Regge Behaviour from an Environmentally Friendly Renormalization Group
The asymptotic behaviour of cubic field theories is investigated in the Regge
limit using the techniques of environmentally friendly renormalization,
environmentally friendly in the present context meaning asymmetric in its
momentum dependence. In particular we consider the crossover between large and
small energies at fixed momentum transfer for a model scalar theory of the type
phi^2 psi. The asymptotic forms of the crossover scaling functions are
exhibited for all two particle scattering processes in this channel to one loop
in a renormalization group improved perturbation theory.Comment: 9 pages text, one figure, LaTeX, uses psfig.sty. Revised version
submitted to Phys. Lett. B., besides minor changes a figure to illustrate the
conventions and a discussion of the full crossover function have been adde
Investigation of the potentialities of photochemical laser systems. Part I - Survey and analysis Final report, 1 Feb. 1966 - 31 Jan. 1967
Photodissociative laser systems used to convert solar radiation to monochromatic coherent emission - excitation mechanisms, spectroscopy of gases absorbing light, and chemical processe
Using variograms to detect and attribute hydrological change
There have been many published studies aiming to identify temporal changes in river flow time series, most of which use monotonic trend tests such as the Mann–Kendall test. Although robust to both the distribution of the data and incomplete records, these tests have important limitations and provide no information as to whether a change in variability mirrors a change in magnitude. This study develops a new method for detecting periods of change in a river flow time series, using temporally shifting variograms (TSVs) based on applying variograms to moving windows in a time series and comparing these to the long-term average variogram, which characterises the temporal dependence structure in the river flow time series. Variogram properties in each moving window can also be related to potential meteorological drivers. The method is applied to 91 UK catchments which were chosen to have minimal anthropogenic influences and good quality data between 1980 and 2012 inclusive. Each of the four variogram parameters (range, sill and two measures of semi-variance) characterise different aspects of the river flow regime, and have a different relationship with the precipitation characteristics. Three variogram parameters (the sill and the two measures of semi-variance) are related to variability (either day-to-day or over the time series) and have the largest correlations with indicators describing the magnitude and variability of precipitation. The fourth (the range) is dependent on the relationship between the river flow on successive days and is most correlated with the length of wet and dry periods. Two prominent periods of change were identified: 1995–2001 and 2004–2012. The first period of change is attributed to an increase in the magnitude of rainfall whilst the second period is attributed to an increase in variability of the rainfall. The study demonstrates that variograms have considerable potential for application in the detection and attribution of temporal variability and change in hydrological systems
Subwavelength localization and toroidal dipole moment of spoof surface plasmon polaritons
We experimentally and theoretically demonstrate subwavelength scale localization of spoof surface plasmon polaritons at a point defect in a two-dimensional groove metal array. An analytical expression for dispersion relation of spoof surface plasmon polaritons substantiates the existence of a band gap where a defect mode can be introduced. A waveguide coupling method allows us to excite localized spoof surface plasmon polariton modes and measure their resonance frequencies. Numerical calculations confirm that localized modes can have a very small modal volume and a high Q factor both of which are essential in enhancing light-matter interactions. Interestingly, we find that the localized spoof surface plasmon polariton has a significant toroidal dipole moment, which is responsible for the high Q factor, as well as an electric quadrupole moment. In addition, the dispersion properties of spoof surface plasmon polaritons are analyzed using a modal expansion method and numerical calculations
Local density of states in the vortex lattice in a type II superconductor
Local density of states (LDOS) in the triangular vortex lattice is
investigated based on the quasi-classical Eilenberger theory. We consider the
case of an isotropic s-wave superconductor with the material parameter
appropriate to NbSe_2. At a weak magnetic field, the spatial variation of the
LDOS shows cylindrical structure around a vortex core. On the other hand, at a
high field where the core regions substantially overlap each other, the LDOS is
sixfold star-shaped structure due to the vortex lattice effect. The orientation
of the star coincides with the experimental data of the scanning tunneling
microscopy. That is, the ray of the star extends toward the nearest-neighbor
(next nearest-neighbor) vortex direction at higher (lower) energy.Comment: 10 pages, RevTex, 32 figure
X-ray absorption spectroscopy on layered cobaltates Na_xCoO_2
Measurements of polarization and temperature dependent soft x-ray absorption
have been performed on Na_xCoO_2 single crystals with x=0.4 and x=0.6. They
show a deviation of the local trigonal symmetry of the CoO_6 octahedra, which
is temperature independent in a temperature range between 25 K and 372 K. This
deviation was found to be different for Co^{3+} and Co^{4+} sites. With the
help of a cluster calculation we are able to interpret the Co L_{23}-edge
absorption spectrum and find a doping dependent energy splitting between the
t_{2g} and the e_g levels (10Dq) in Na_xCoO_2.Comment: 7 pages, 8 figure
Absence of Dipole Transitions in Vortices of Type II Superconductors
The response of a single vortex to a time dependent field is examined
microscopically and an equation of motion for vortex motion at non-zero
frequencies is derived. Of interest are frequencies near ,
where is the bulk energy gap and is the fermi energy. The low
temperature, clean, extreme type II limit and maintaining of equilibrium with
the lattice are assumed. A simplification occurs for large planar mass
anisotropy. Thus the results may be pertinent to materials such as and
high temperature superconductors. The expected dipole transition between core
states is hidden because of the self consistent nature of the vortex potential.
Instead the vortex itself moves and has a resonance at the frequency of the
transition.Comment: 12 pages, no figure
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