1,134 research outputs found
rRNA gene activity and control of expression mediated by methylation and imprinting during embryo development in wheat x rye hybrids
Ribosomal RNA genes originating from one
parent are often suppressed in interspecific hybrids. We
show that treatments during germination with the
cytosine analogue 5-azacytidine stably reactivate the
expression of the suppressed rRNA genes of rye origin in
the wheat x rye amphiploid, triticale, by preventing
methylation of sites in the rye rDNA. When 5-azacytidine
is applied to embryos of triticale and
wheat x rye F1 hybrids nine, or more, days after fertilization,
rye rRNA gene expression is stably reactivated in
the resulting seedling. Earlier treatments have no effect
on rye rRNA gene expression, indicating that undermethylation
of DNA early in embryo development is
reversible. After 9 days, the methylation status of rRNA
genes in maintained throughout development. Since the
change in expression follows a methylation change at
particular restriction-enzyme sites, the data establish a
clear correlation between gene activity and methylation
in plants
The meaning of maxima and minima in first order reversal curves: Determining the interaction between species in a sample
First-order reversal curves (FORCs) are a characterization technique for magnetic materials used in a wide range of research fields. Since their first application in the Earth Sciences two decades ago, their importance in science has been continuously growing and new experimental techniques have been subsequently designed based on the original idea of FORCs. Nonetheless, very recent experimental works on very well designed and simple magnetic structures demonstrate that even for the most simple cases the interpretation of FORC data lacks understanding. In this work, we address this problem analytically, explaining the meaning of maxima, minima and noisy tails and set a strategy to extract the interaction field between magnetic structures. The origin of this interaction field is often the magnetostatic energy, however, we propose that this strategy could be applied for estimating exchange interactions too
ECONOMIC BENEFITS OF WEATHER PROTECTION FOR LARGE ROUND BALES
. 1986. Economic benefits of weather protection for large round bales. Can. An economic evaluation ofthe storage losses ofvarious haying systems isreported. This information was necessary to augment a study of the effect of storage characteristicson round bale quality in which round bales from two different round baler types (fixed and variable bale chamber) were stored in five different manners. In addition, the costs of harvesting and storing hay by several methods were evaluated. These costs were translated into costs per "useable" tonneof hay. Values of spoiledhay were compared with the values of weather protection for round bales. The values ofhay spoilage indifferent types of storage were combined with detailed costs of growing, harvesting and storing hay and compared. Economic comparison with traditional rectangular bale harvesting methods is also included. It was found that round bale harvesting was the least expensive method at all volumes. Round bale harvesting with inside storage or plastic wrap protection provided a higher value than outside storage of round bales because of the increased spoilage when not protected. The cost of inside storage was equal to 14% spoilage of round bales
New methods for unmixing sediment grain size data
Grain size distribution (GSD) data are widely used in Earth sciences and although large data sets are regularly generated, detailed numerical analyses are not routine. Unmixing GSDs into components can help understand sediment provenance and depositional regimes/processes. End-member analysis (EMA), which fits one set of end-members to a given data set, is a powerful way to unmix GSDs into geologically meaningful parts. EMA estimates end-members based on covariability within a data set and can be considered as a nonparametric approach. Available EMA algorithms, however, either produce suboptimal solutions or are time consuming. We introduce unmixing algorithms inspired by hyperspectral image analysis that can be applied to GSD data and which provide an improvement over current techniques. Nonparametric EMA is often unable to identify unimodal grain size subpopulations that correspond to single sediment sources. An alternative approach is single-specimen unmixing (SSU), which unmixes individual GSDs into unimodal parametric distributions (e.g., lognormal). We demonstrate that the inherent nonuniqueness of SSU solutions renders this approach unviable for estimating underlying mixing processes. To overcome this, we develop a new algorithm to perform parametric EMA, whereby an entire data set can be unmixed into unimodal parametric end-members (e.g., Weibull distributions). This makes it easier to identify individual grain size subpopulations in highly mixed data sets. To aid investigators in applying these methods, all of the new algorithms are available in AnalySize, which is GUI software for processing and unmixing grain size data
Addressing School Related Gender Based Violence in Zambia: A Scoping Study
This report presents findings from a scoping study of policy, practice and evidence on school-related
gender-based violence (SRGBV) in Zambia, which was carried out in 2016. The main objective of the
study was to analyse responses to gender-based violence in and around schools in Zambia, in order to
inform future planning of policy and practice initiatives. The study was a collaboration between the
government of Zambia, UNICEF, and researchers at the UCL Institute of Education working alongside
consultant, Romana Maumbu. Its core elements consist of: analysis of legislation and policy; analysis
of programming on SRGBV; mapping of stakeholders working on SRGBV; and the identification and
evaluation of research and data sets. The findings presented here will be used to guide decision
making for phase two of the initiative which will take place during 2017, as well as longer term
planning and action on SRGBV in Zambia. The findings will provide the basis for reflection and the
development of the action plan for the next phase of the EGVS initiative
What Brown saw and you can too
A discussion is given of Robert Brown's original observations of particles
ejected by pollen of the plant \textit{Clarkia pulchella} undergoing what is
now called Brownian motion. We consider the nature of those particles, and how
he misinterpreted the Airy disc of the smallest particles to be universal
organic building blocks. Relevant qualitative and quantitative investigations
with a modern microscope and with a "homemade" single lens microscope similar
to Brown's, are presented.Comment: 14.1 pages, 11 figures, to be published in the American Journal of
Physics. This differs from the previous version only in the web site referred
to in reference 3. Today, this Brownian motion web site was launched, and
http://physerver.hamilton.edu/Research/Brownian/index.html, is now correc
Enseignement de l'échographie au chevet à l'aide d'une technique de visioconférence
Implication Statement
Point-of-care ultrasound (POCUS) has usually been taught using a hands-on, in-person approach. We present a novel approach to delivering POCUS virtually using a dual image videoconferencing technique. We outline an easily implementable approach and summarize medical studentsâ experience and feedback. This form of delivery has potential to improve instructional delivery in resource restricted settings or during pandemic restrictions where a hands-on approach may not be possible.ĂnoncĂ© des implications de la recherche
L'Ă©chographie au chevet fait gĂ©nĂ©ralement lâobjet dâun enseignement pratique, en personne. Nous prĂ©sentons une nouvelle approche, virtuelle, pour son enseignement, par visioconfĂ©rence Ă double flux vidĂ©o. Lâapproche que nous dĂ©crivons est facile Ă mettre en Ćuvre. Nous rĂ©sumons l'expĂ©rience et les commentaires des Ă©tudiants en mĂ©decine sur cette modalitĂ© qui est susceptible d'amĂ©liorer l'enseignement dans des contextes oĂč les ressources sont limitĂ©es ou en cas de pandĂ©mie, lorsque lâapproche pratique n'est pas possible
Fermi surface and order parameter driven vortex lattice structure transitions in twin-free YBa2Cu3O7
We report on small-angle neutron scattering studies of the intrinsic vortex
lattice (VL) structure in detwinned YBa2Cu3O7 at 2 K, and in fields up to 10.8
T. Because of the suppressed pinning to twin-domain boundaries, a new distorted
hexagonal VL structure phase is stabilized at intermediate fields. It is
separated from a low-field hexagonal phase of different orientation and
distortion by a first-order transition at 2.0(2) T that is probably driven by
Fermi surface effects. We argue that another first-order transition at 6.7(2)
T, into a rhombic structure with a distortion of opposite sign, marks a
crossover from a regime where Fermi surface anisotropy is dominant, to one
where the VL structure and distortion is controlled by the order-parameter
anisotropy.Comment: 4 pages, 3 figures (2 color), minor change
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