Vocal universals and geographic variations in the acoustic repertoire of the common bottlenose dolphin

Acoustical geographic variation is common in widely distributed species and it is already described for several taxa, at various scales. In cetaceans, intraspecific variation in acoustic repertoires has been linked to ecological factors, geographical barriers, and social processes. For the common bottlenose dolphin (Tursiops truncatus), studies on acoustic variability are scarce, focus on a single signal type-whistles and on the influence of environmental variables. Here, we analyze the acoustic emissions of nine bottlenose dolphin populations across the Atlantic Ocean and the Mediterranean Sea, and identify common signal types and acoustic variants to assess repertoires' (dis)similarity. Overall, these dolphins present a rich acoustic repertoire, with 24 distinct signal sub-types including: whistles, burst-pulsed sounds, brays and bangs. Acoustic divergence was observed only in social signals, suggesting the relevance of cultural transmission in geographic variation. The repertoire dissimilarity values were remarkably low (from 0.08 to 0.4) and do not reflect the geographic distances among populations. Our findings suggest that acoustic ecology may play an important role in the occurrence of intraspecific variability, as proposed by the 'environmental adaptation hypothesis'. Further work may clarify the boundaries between neighboring populations, and shed light into vocal learning and cultural transmission in bottlenose dolphin societies.Fundação para a Ciência e Tecnologia - FCTinfo:eu-repo/semantics/publishedVersio

Aromaticity in a Surface Deposited Cluster: Pd4_4 on TiO2_2 (110)

We report the presence of \sigma-aromaticity in a surface deposited cluster, Pd$_4$ on TiO$_2$ (110). In the gas phase, Pd$_4$ adopts a tetrahedral structure. However, surface binding promotes a flat, \sigma-aromatic cluster. This is the first time aromaticity is found in surface deposited clusters. Systems of this type emerge as a promising class of catalyst, and so realization of aromaticity in them may help to rationalize their reactivity and catalytic properties, as a function of cluster size and composition.Comment: 4 pages, 3 figure

Hydrochlorination of acetylene using supported bimetallic Au-based catalysts

A detailed study of the hydrochlorination of acetylene and higher alkynes using a supported gold catalyst is described and discussed. A series of reactions using sequential exposure of the catalysts to C2H2 and HCl demonstrate that exposure to HCl prior to reaction of C2H2/HCl leads to enhanced activity whereas exposure to C2H2 leads to deactivation. The reaction of higher alkynes is affected by steric factors with the trend in activity being: acetylene (ca. 40 % conversion)>> hex-1-yne (10%)>phenylacetylene (7 %) > hex-2-yne (2 %) under standard reaction conditions. Using 1H-NMR spectroscopy we have found that for hex-1-yne and phenyl acetylene the anti-Markovnikov product is formed by anti addition of HCl. However, the Markovnikov products are equivalent for syn- and antiaddition of HCl, and hence we investigated the reaction using deuterated substrates and confirmed the products are formed by the anti addition of HCl. The reaction mechanism is discussed in detail

Microscopic investigation of single-wall carbon nanotube uptake by Daphnia magna

The objectives of this study were to determine the extent of absorption of functionalized single-wall carbon nanotubes (SWCNTs) across the gut epithelial cells in Daphnia magna. Several microscopic techniques were utilized, including micro-Raman spectroscopy, high-resolution transmission electron microscopy (HRTEM) and selective area diffraction (SAD). In an effort to examine the variation in uptake due to surface properties, four groups of differently functionalized SWCNTs were used: hydroxylated (OH-SWCNTs), silicon dioxide (SiO₂-SWCNTs), poly aminobenzenesulfonic acid (PABS-SWCNTs) and polyethylene glycol (PEG-SWCNTs). Raman spectroscopy was able to detect OH-SWCNTs within the gut, but lacked the spatial resolution that is needed to identify lower concentrations of SWCNTs that may have been absorbed by body tissues. Initially, low-magnification imaging of exposed D. magna sections in the TEM revealed several features, which suggested absorption of SWCNTs. However, subsequent analysis with additional techniques (HRTEM, X-ray energy-dispersive spectroscopy and SAD) indicated that these features were either artifacts produced via the specimen staining process or consisted of non-graphitic, organic structures. This latter observation emphasizes the inherent difficulty in resolving SWCNTs embedded within a complex, organic matrix, as well as the care with which imaging results must be interpreted and supplemented with other, more analytical techniques.Keywords: Nanomaterials, Electron microscopy, Absorptio

Do Porpoises Choose Their Associates? A New Method for Analyzing Social Relationships among Cetaceans

BACKGROUND: Observing and monitoring the underwater social interactions of cetaceans is challenging. Therefore, previous cetacean studies have monitored these interactions by surface observations. However, because cetaceans spend most of their time underwater, it is important that their underwater behavior is also continuously monitored to better understand their social relationships and social structure. The finless porpoise is small and has no dorsal fin. It is difficult to observe this species in the wild, and little is known of its sociality. METHODOLOGY/PRINCIPAL FINDINGS: The swim depths of 6 free-ranging finless porpoises were simultaneously recorded using a time-synchronized bio-logging system. Synchronous diving was used as an index of association. Two pairs, #27 (an immature female estimated to be 3.5 years old) and #32 (an adult male), #28 (a juvenile male estimated to be 2 years old) and #29 (an adult male), tended to participate in long periods of synchronized diving more frequently than 13 other possible pairs, indicating that the 4 porpoises chose their social partners. The adult males (#32, #29) tended to follow the immature female (#27) and juvenile male (#28), respectively. However, during synchronized diving, the role of an initiator often changed within the pair, and their body movements appeared to be non-agonistic, e.g., rubbing of bodies against one another instead of that on one-side, as observed with chasing and escaping behaviors. CONCLUSIONS/SIGNIFICANCE: The present study employed a time-synchronized bio-logging method to observe the social relationships of free-ranging aquatic animals based on swimming depth. The results suggest that certain individuals form associations even if they are not a mother and calf pair. Long synchronized dives occurred when particular members were reunited, and this suggests that the synchronized dives were not a by-product of opportunistic aggregation

Sex bias in biopsy samples collected from free-ranging dolphins

Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in European Journal of Wildlife Research 56 (2010): 151-158, doi:10.1007/s10344-009-0299-7.Biological samples of free-ranging dolphins are increasingly used to gain information on population structure and ecology. In small cetaceans, the gender of individuals usually cannot be determined at sea, and population sex ratio has to be inferred indirectly. We used molecular sexing to determine the gender of 340 biopsy samples of bottlenose dolphins, Tursiops truncatus, spotted dolphins, Stenella frontalis, and common dolphins, Delphinus delphis, collected around the Azores and Madeira. Sex ratio was globally skewed in favor of males, and differed between species and archipelagos. Skew was probably influenced by the selectivity of biopsy collectors and seasonal or year-round predominance of males in natural populations. Skew was also influenced by sampling duration and intensity. In the Azores, when several samples were successively collected within the same group, the proportion of female samples decreased as a function of sample order. This trend indicated a tendency for females to increasingly avoid the boat while samples were being collected. It showed that males and females reacted differently to the perturbation caused by the biopsy sampling process (i.e. sample collection and driving style).Portuguese Foundation for Science and Technology (FCT) and the FEDER program for funding the CETAMARH (POCTI/BSE/38991/01) and the GOLFINICHO (POCI/BIA-BDE/61009/2004) projects, S.Q.'s post-doctoral grants (IMAR/FCT- PDOC-006/2001-MoleGen and SFRH/BPD/19680/2004), M.A.S.'s doctoral (SFRH/BD/8609/2002) and post-doctoral (SFRH/BPD/29841/2006) grants, S.M.'s investigation assistant grant (CETAMARHII/POCTI/BSE/38991/2001) and I.C.'s investigation assistant grants (IMAR/FCT/GOLFINICHO/001/2005 and IMAR/FCT/GOLFINICHO/004/2006). FCT for its pluri-annual funding to Research Unit #531 and the EU funded program Interreg IIIb for funding the MACETUS project (MAC/4.2/M10) as well as R.P. and S.M.’s grants (IMAR/INTERREGIIIb/MACETUS/MAC1/2)