The Use of Grape Seed Byproducts Rich in Flavonoids to Improve the Antioxidant Potential of Red Wines

The influence of adding seeds from grape pomace during Syrah wine fermentation in a warm climate has been studied. Seeds of Pedro Ximenez variety were rich in phenolic compounds, mainly flavonoids such as catechin and procyanidins. Changes in total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of red wines were observed. These changes depended on the vinification stage and the amount of seeds (SW: 450 g or DW: 900 g seeds/150 kg grapes) applied. In general, antioxidant activity was greater when a simple dose (SW) was considered. Results indicate that seeds rich in flavonoids could be used as wine additives, which could improve the antioxidant potential of red wines in a warm climate.Ministerio de Economía y Competitividad AGL2014-58486-C2-2-

Upgrade of goniospectrophtometer GEFE for near-field scattering and fluorescence radiance measurements

Proceedings of SPIE 9398; San Francisco, California, United States | February 8-9, 2015The goniospectrophotometer GEFE, designed and developed at IO¿CSIC (Instituto de ¿ Optica, Agencia Estatal Consejo Superior de Investigaciones Cient¿¿ficas), was conceived to measure the spectral Bidirectional Reflectance Distribution Function (BRDF) at any pair of irradiation and detection directions. Although the potential of this instrument has largely been proved, it still required to be upgraded to deal with some important scattering features for the assessment of the appearance. Since it was not provided with a detector with spatial resolution, it simply could not measure spectrophotometric quantites to characterize texture through the Bidirectional Texture Function (BTF) or translucency through the more complex Bidirectional Scattering¿Surface Reflectance Distribution Function (BSSRDF). Another requirement in the GEFE upgrading was to provide it with the capability of measuring fluorescence at different geometries, since some of the new pigments used in industry are fluorescent, which can have a non¿negligible impact in the color of the product. Then, spectral resolution at irradiation and detection had to be available in GEFE. This paper describes the upgrading of the goniospectrophotometer GEFE, and its new capabilities through the presentation of sparkle and goniofluorescence measurements. In addition, the potential of the instrument to evaluate translucency by the measurement of the BSSRDF is briefly discussed.Authors are grateful to EMRP for funding the project “Multidimensional reflectometry for industry”. The EMRP is jointly funded by the EMRP participating countries within EURAMET and the European Union. Authors are also grateful to Comunidad de Madrid for funding the project SINFOTON-CM: S2013/MIT-2790.Peer Reviewe

Applications of Voltammetric Analysis to Wine Products

Wine contains polyphenols that are responsible for its quality. Moreover, phenolic compounds have antioxidant properties and benefits on human health. Cyclic voltammetry (CV) was the first electrochemical method used for polyphenols characterization and determination of polyphenols content in wine products. Electrochemical behaviour of standard solutions of phenolic compounds has been investigated and evaluated the importance of the phenolic concentration and pH. The electrochemical parameters extracted from the voltammograms have been correlated with the antioxidant potential in wine products. In addition, CV allowed establishing differences in the antioxidant activity of wines with different addition of grape seeds. In winemaking by-products, different Ipa and Q500 values were found depending on the state of maturation of the grape pomace. On the other hand, the total flavonoids and phenolic acids contents were significantly correlated to the electrochemical parameters. Differences for the electrochemical parameters were found between by-products, being pomace and seeds which presented the greatest values of Q500. Simple regression analyses showed that voltammetric parameters are correlated to their values of lipid peroxidation inhibition by thiobarbituric acid reactive substances method. Our results open the possibility of CV as a promising technique to estimate the global antioxidant potential of wine products rich in phenolic compounds

Potential of Cooperage Byproducts Rich in Ellagitannins to Improve the Antioxidant Activity and Color Expression of Red Wine Anthocyanins

Cooperage byproducts are an important source of phenolic compounds that could be used for wine technology applications. The effects of the addition of two types of oak wood shavings (American, AOW, and Ukrainian, UOW) on the antioxidant activity and color of red wine anthocyanins, in a wine model solution, were evaluated by spectrophotometric and colorimetric analyses. Phenolic compounds from shavings, mainly ellagitannins, were determined by ultra-high-performance liquid chromatography/mass spectrometry (UHPLC/MS). Antioxidant and copigmentation effects varied depending on the type of shavings (AOW and UOW) and the phenolic concentration (100, 400, and 500 mg/L). Phenolic compounds from shavings improved the color characteristics (darker and more bluish color) and the copigmentation effect of red wine anthocyanins, being UOW a better source of copigments than AOW shavings. The best antioxidant activity was found for the 400 and 500 mg/L model solutions for both types of shavings. Results show a winemaking technological application based on the repurposing of cooperage byproducts, which could improve color and antioxidant characteristics of red wines.España, Ministerio de Economía y Competitividad Project AGL2017-84793-C2-2-REspaña, Universidad de Sevilla VI Plan Propio de Investigación y Transferencia (Acción I.5

Determination of phenolic substances of seeds, skins and stems from white grape marc by near-infrared hyperspectral imaging

Background and Aims:Winemaking generates a large quantity of grape marc that causes environmental and economic problems, which could be minimised by exploiting and adding value to these by-products. Marc is composed of seeds, skins and stems that are an important source of phenolic substances, which have antioxidant properties and potential benefits to human health. It is necessary to develop novel methods for assessing the composition of marc because conventional analyses are destructive and require lengthy preparation procedures and chemical reagents. Methods and Results: Near-infrared hyperspectral imaging has been used for evaluating 27 individual phenolic substances in the components of grape marc after freeze-drying, the only sample preparation step. By applying partial least squares regression to the spectral data, values of R2 up to 0.98 were obtained even for estimating some minor compounds. Conclusions: This methodology arises as an attractive alternative to analyse the phenolic composition of grape marc because of its speed and simplicity. Significance of the Study: The use of this technique will allow wine producers to quickly determine the phenolic composition and decide the destination of this by-produc

Parámetros relevantes en el estudio de la no-linealidad de fotodiodos

OPTOEL 2015, Salamanca 13 a 15 de julio de 2015; http://optoel2015.usal.es/El comportamiento lineal en la respuesta de un fotodiodo es una condición fundamental en medidas radiométricas, espectrofotométricas y de radiación pulsada o continua. Por tanto, se requiere que la respuesta eléctrica del fotodiodo sea proporcional al flujo radiante incidente y que se mantenga estable en el tiempo y en un rango de irradiancia. Sin embargo, diversas medidas publicadas evidencian una falta de linealidad dependiente de la estructura interna del fotodiodo, de la potencia y del tamaño del haz incidente. Este trabajo, para caracterizar la no-linealidad en la respuesta de fotodiodos como función de estos mismos factores, adopta el modelo de eficiencia cuántica interna desarrollado por Ferrero et al. en el Instituto de Óptica (CSIC). Además, mediante simulaciones para distintos supuestos, evalúa la influencia de cada parámetro en la no-linealidad de respuesta comparando resultados de las simulaciones con datos experimentales seleccionados en una recopilación bibliográfica.Los autores agradecen al EMRP la subvención del proyecto NEWSTAR. El programa EMRP está financiado conjuntamente por los países de EURAMET participantes y la Unión Europea.Peer Reviewe

Effect of the time of cold maceration on the evolution of phenolic compounds and colour of Syrah wines elaborated in warm climate

The effect of different cold maceration times on Syrah red wines from warm climate has been evaluated. Differential colorimetry and tristimulus colorimetry were applied to colour data at different points of the vinification stage. Virtually, all the phenolic compounds showed significantly higher content in cold macerated wines, even more when longer contact time was used. Long cold macerated (LCM) wines had the significantly (P < 0.05) highest chroma values (C*ab) and lowest of lightness (L*) and hue (hab). Unexpectedly, wines submitted to short-time cold maceration (SCM) presented the lowest chroma values, even significantly inferior to that observed in traditional macerated (TM) wines. Moreover, colour changes (DE*ab) between TM and SCM were mainly quantitative (%D2L and %D2C), whereas they were also qualitative when compared TM with LCM (%D2C and %D2H). Cold maceration in warm climates characterised by common colour losses must be carefully employed because undesirable consequences on colour may occur.Consejería de Innovación, Ciencia y Empresa (Junta de Andalucía, Spain) project P-10- AGR-06331Ministerio de Ciencia y Tecnología (Spain) project AGL2003-0297

Spanish facilities and fisrst results in measuring thermodynamic temperature using the radiance method

Newport Pagnell, Buckinghamshire, May 18-19 2015; https://royalsociety.org/science-events-and-lectures/2015/05/new-kelvin/Currently, the dissemination of the kelvin, according to the International Temperature Scale (ITS-90), at high temperatures is realised at the Centro Español de Metrología (CEM) by using the fixed points of Ag and Cu and a standard radiation thermometer. Recently, absolute radiometry has been proposed by the CCT Working Group 5 [1] to be included in future revisions of the Mise-en-Practique for the kelvin (MeP-K). The Centro Español de Metrologia (CEM) in collaboration with the Instituto de Óptica of the Consejo Superior de Investigaciones Científicas (IO-CSIC) has been working in the following lines linked to this new alternative to disseminate the kelvin: ¿ Absolute calibration, i.e., calibration in terms of the thermodynamic temperature, of commercial radiation thermometers, by using a facility based on a monochromator and a high stability lamp [2]. At present, the uncertainties of these measurements, obtained with a KE-LP2 radiation thermometer, are about the ones obtained with the ITS-90 method, i. e. from 0,3 K to 1,1 K (from 1084,62 °C to 2474 °C, k = 2). Some improvements in the facilities described in [2] have been implemented in order to reduce uncertainty. ¿ Development of a filter radiometer to measure the thermodynamic temperature. It has been designed by CEM based in NPL previous work [3]. This filter radiometer can be calibrated with a laser based experimental setup, which improves the above measurement uncertainty [4]: from 0,2 K to 0,7 K (from 1084,62 °C to 2474 °C, k = 2). Because of some problems with the radiometer stability at 2500 °C, some changes in the design have been done. The facilities used in [2] and [4] are currently been developed at CEM site as the transportation of the instruments for calibration has caused significant drifts. A complete description of IO-CSIC and new CEM facilities will be presented, together with the results obtained on the determination of the WP1 InK cells thermodynamic temperatures by using both, a radiation thermometer with an absolute calibration, and a filter radiometer.Peer Reviewe

Assessment of white grape pomace from winemaking as source of bioactive compounds, and its antiproliferative activity

The antiproliferative effects of a purified white grape pomace extract (PWGPE), as well as of some phenolic standards on colon cancer cells were examined. The phenolic composition of the PWGPE was determined by rapid resolution liquid chromatography/mass spectrometry (RRLC/MS). The PWGPE had 92.6, 43.3 and 6.01 mg/g of flavanols, flavonols and phenolic acids, respectively and, along with pure catechin, epicatechin, quercetin and gallic acid, they were all found capable of inhibiting cellular proliferation. PWGPE (100 lg/ml) inhibited the proliferation of cells by 52.1% at 48 h, whilst catechin, epicatechin, quercetin and gallic acid (60 lg/ml) inhibited growth by 65.2%, 62.2%, 81.0% and 71.0%, respectively, at 72 h. The PWGPE is an interesting source of phenolic compounds with antiproliferative properties, that could be of interest in the food and pharmaceutical industries

Multi dimensional comparisons

Consultative Committee for Photometry and Radiometry (CCPR) Workshop. China 26 October 2015How to asses measurement comparisons of multidimensional quantities? An approach is presented in this workPeer Reviewe