28 research outputs found

    Outcomes from elective colorectal cancer surgery during the SARS-CoV-2 pandemic

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    This study aimed to describe the change in surgical practice and the impact of SARS-CoV-2 on mortality after surgical resection of colorectal cancer during the initial phases of the SARS-CoV-2 pandemic

    TPK and cultivated species

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    The dataset includes the different taxa reported in each survey. This information served as a reference for the analyses of plant use in the community. The following sheets include the presence of cultivated species for both those plots that participated in the PAR project and those that did not. A summary of the descriptive statistics of the comparison of species richness between the different plots analysed is also included

    TPK and cultivated species

    No full text
    The dataset includes the different taxa reported in each survey. This information served as a reference for the analyses of plant use in the community. The following sheets include the presence of cultivated species for both those plots that participated in the PAR project and those that did not. A summary of the descriptive statistics of the comparison of species richness between the different plots analysed is also included

    Savinase, the most suitable enzyme for releasing peptides from lentil (Lens culinaris var. Castellana) protein concentrates with multifunctional properties

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    The aim of this study was to produce multifunctional hydrolysates from lentil protein concentrates. Four different proteases (Alcalase, Savinase, Protamex, and Corolase 7089) and different hydrolysis times were evaluated for their degree and pattern of proteolysis and their angiotensin I-converting enzyme (ACE) inhibitory and antioxidant activities. Alcalase and Savinase showed the highest proteolytic effectiveness (P ¿ 0.05), which resulted in higher yield of peptides. The hydrolysate produced by Savinase after 2 h of hydrolysis (S2) displayed the highest ACE-inhibitory (IC50 = 0.18 mg/mL) and antioxidant activity (1.22 ¿mol of Trolox equiv/mg of protein). Subsequent reverse-phase HPLC-tandem mass spectrometric analysis of 3 kDa permeates of S2 showed 32 peptides, mainly derived from convicilin, vicilin, and legumin containing bioactive amino acid sequences, which makes them potential contributors to ACE-inhibitory and antioxidant activities detected. The ACE-inhibitory and antioxidant activities of S2 were significantly improved after in vitro gastrointestinal digestion (P ¿ 0.05). Multifunctional hydrolysates could encourage value-added utilization of lentil proteins for the formulation of functional foods and nutraceuticals. © 2014 American Chemical Society.This work was funded by the AGL2010-16310 project from the MINECO (Ministerio de Economía y Competitividad from Spain).Peer Reviewe

    Novel Antihypertensive Lactoferrin-Derived Peptides Produced by Kluyveromyces marxianus

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    Novel antihypertensive peptides released by Kluyveromyces marxianus from bovine lactoferrin (LF) have been identified. K. marxianus LF permeate was fractionated by semipreparative high performance liquid chromatography and 35 peptides contained in the angiotensin I-converting enzyme (ACE)-inhibitory fractions were identified by using an ion trap mass spectrometer. On the basis of peptide abundance and common structural features, six peptides were chemically synthesized. Four of them (DPYKLRP, PYKLRP, YKLRP, and GILRP) exerted in vitro inhibitory effects on ACE activity and effectively decreased systolic blood pressure after oral administration to spontaneously hypertensive rats (SHRs). Stability against gastrointestinal enzymes suggested that the sequence LRP could contribute to the in vivo effects of parental peptides. Finally, there were reductions in circulating ACE activity and angiotensin II level in SHRs after either DPYKLRP or LRP intake, thus confirming ACE inhibition as the in vivo mechanism for their antihypertensive effect.This work was supported by grant AGL2010–21009 from ‘Ministerio de Educación y Ciencia - FEDER’, Consolider Ingenio 2010, Fun-C-Food, CSD2007–00063 and RETICS INVICTUS RD12/0014/0004 from ‘Instituto de Salud Carlos III’. A.G.-T. is the recipient of a predoctoral fellowship from ‘Ministerio de Educación y Ciencia’ (BES-2011–044424).Peer reviewe
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