Feeding Tests with Indigofera endecaphylla Jacq. (Creeping Indigo) and Some Observations on Its Poisonous Effects on Domestic Animals

Leguminous plants have long been considered important as livestock feeds in Hawaii. They decrease the need for costly oil cakes and protein rich concentrates in milk and meat production. Indigofera endecapliylla Jacq. (creeping indigo or trailing indigo) seemed promising for a time as a high-rainfall-zone legume. Early experiments proved that it would grow well with a wide variety of associated grasses, and grazing tests showed that it was palatable and quite persistent under pasture conditions. Very little was known, on the other hand, of its feeding value for livestock. In 10 years of short-interval pasture trials with relatively small proportions of the legume, no adverse effects were noted on young cattle. However, when the concentration of the legume exceeded about 50 per cent of the forage, toxicity symptoms began to appear. A study of the effect of a strain of Indigofera endecapliylla Jacq. grown in Hawaii and tested as a feed for cows, heifers, sheep, and rabbits is presented

The validity, responsiveness, and score interpretation of the PROMISnq Physical Function – Multiple Sclerosis 15a short form in multiple sclerosis

© 2022 Published by Elsevier B.V.Background A valid, sensitive patient-reported outcome (PRO) measure of physical function (PF) for people with multiple sclerosis (MS) would have substantial value in routine care and clinical research. We now describe development of the PROMISnq Short Form v2.0 PF – Multiple Sclerosis 15a [PROMISnq PF(MS)15a] for assessing PF in relapsing and progressive MS. Also, the validity, reliability, and responsiveness of the PROMISnq PF(MS)15a is evaluated, minimal important difference (MID) thresholds for score change estimated and a score interpretation guide developed. Methods A mixed-methods sequential design was employed. Relevant PF concepts were elicited through semi-structured interviews with people with relapsing MS, and then mapped to the PROMIS PF item bank. Measurement experts integrated results from interviews with people with MS and input from a panel of neurologists to generate a draft short form. Relevance and comprehensiveness of the draft short form were assessed in cognitive debriefing interviews with people with relapsing or progressive MS. Subsequently, item reduction and evaluation of psychometric properties were performed in two observational studies: a cross-sectional study in the US (n = 296), and a 96-week longitudinal study in the UK MS Register cohort (n = 558). The main outcomes and measures are estimates of: known-groups validity, convergent validity, reliability, responsiveness; MID for worsening. Results Factor analyses supported the unidimensionality of the newly derived 15-item short form. Cronbach's alpha (≥ 0.97) and intraclass correlation coefficient (≥ 0.97) of test-retest scores (5–27 days) indicated strong reliability. Convergent validity was demonstrated by moderate-to-strong correlations with scores on related PRO measures. Scores discriminated among patient groups classified by levels of physical health and other criteria. Score changes of 2.3–2.7 points are proposed as MID criteria for minimal worsening in PF. Conclusion PROMISnq PF(MS)15a demonstrated reliability, validity and sensitivity to change. Input from patients and clinicians ensured the content is comprehensive and relevant for people with MS.Peer reviewe

Presence of genes for type III secretion system 2 in Vibrio mimicus strains

<p>Abstract</p> <p>Background</p> <p>Vibrios, which include more than 100 species, are ubiquitous in marine and estuarine environments, and several of them e.g. <it>Vibrio cholerae</it>, <it>V. parahaemolyticus</it>, <it>V. vulnificus </it>and <it>V. mimicus</it>, are pathogens for humans. Pathogenic <it>V. parahaemolyticus </it>strains possess two sets of genes for type III secretion system (T3SS), T3SS1 and T3SS2. The latter are critical for virulence of the organism and be classified into two distinct phylogroups, T3SS2α and T3SS2β, which are reportedly also found in pathogenic <it>V. cholerae </it>non-O1/non-O139 serogroup strains. However, whether T3SS2-related genes are present in other <it>Vibrio </it>species remains unclear.</p> <p>Results</p> <p>We therefore examined the distribution of the genes for T3SS2 in vibrios other than <it>V. parahaemolyticus </it>by using a PCR assay targeting both T3SS2α and T3SS2β genes. Among the 32 <it>Vibrio </it>species tested in our study, several T3SS2-related genes were detected in three species, <it>V. cholerae</it>, <it>V. mimicus </it>and <it>V. hollisae</it>, and most of the essential genes for type III secretion were present in T3SS2-positive <it>V. cholerae </it>and <it>V. mimicus </it>strains. Moreover, both <it>V. mimicus </it>strains possessing T3SS2α and T3SS2β were identified. The gene organization of the T3SS2 gene clusters in <it>V. mimicus </it>strains was fundamentally similar to that of <it>V. parahaemolyticus </it>and <it>V. cholerae </it>in both T3SS2α- and T3SS2β-possessing strains.</p> <p>Conclusions</p> <p>This study is the first reported evidence of the presence of T3SS2 gene clusters in <it>V. mimicus </it>strains. This finding thus provides a new insight into the pathogenicity of the <it>V. mimicus </it>species.</p

High gamma oscillations in medial temporal lobe during overt production of speech and gestures

The study of the production of co-speech gestures (CSGs), i.e., meaningful hand movements that often accompany speech during everyday discourse, provides an important opportunity to investigate the integration of language, action, and memory because of the semantic overlap between gesture movements and speech content. Behavioral studies of CSGs and speech suggest that they have a common base in memory and predict that overt production of both speech and CSGs would be preceded by neural activity related to memory processes. However, to date the neural correlates and timing of CSG production are still largely unknown. In the current study, we addressed these questions with magnetoencephalography and a semantic association paradigm in which participants overtly produced speech or gesture responses that were either meaningfully related to a stimulus or not. Using spectral and beamforming analyses to investigate the neural activity preceding the responses, we found a desynchronization in the beta band (15-25 Hz), which originated 900 ms prior to the onset of speech and was localized to motor and somatosensory regions in the cortex and cerebellum, as well as right inferior frontal gyrus. Beta desynchronization is often seen as an indicator of motor processing and thus reflects motor activity related to the hand movements that gestures add to speech. Furthermore, our results show oscillations in the high gamma band (50-90 Hz), which originated 400 ms prior to speech onset and were localized to the left medial temporal lobe. High gamma oscillations have previously been found to be involved in memory processes and we thus interpret them to be related to contextual association of semantic information in memory. The results of our study show that high gamma oscillations in medial temporal cortex play an important role in the binding of information in human memory during speech and CSG production

Effects of anthropogenic structures and a wetland on macroinvertebrate diversity and water chemistry along the Maple River.

General EcologyAquatic systems play an integral role in wide ranging ecological contexts by providing an outlet of nutrient and chemical buildup. Rivers are particularly vulnerable to deleterious anthropogenic effects given their popularity as transit and recreation waterways. The health of a stream can accurately be assessed using an index of macroinvertebrate diversity as well as a battery of chemical concentration tests. Our study found that the Maple River shows few negative effects of anthropogenic structures on macroinvertebrate health and on overall stream health. The presence of a wetland may also have served to mitigate the possible human effects.http://deepblue.lib.umich.edu/bitstream/2027.42/96208/1/Alanouf_Cote_Henke_Hsieh_2012.pd

Formal verification for fault-tolerant architectures: Prolegomena to the design of PVS

Abstract-- PVS is the most recent in a series of verification systems developed at SRI. Its design was strongly influenced, and later refined, by our experiences in developing formal specifications and mechanically checked verifications for the fault-tolerant architecture, algorithms, and implementations of a model &quot;reliable computing platform &quot; (RCP) for life-critical digital flight-control applications, and by a collaborative project to formally verify the design of a commercial avionics processor called AAMP5. Several of the formal specifications and verifications performed in support of RCP and AAMP5 are individually of considerable complexity and difficulty. But in order to contribute to the overall goal, it has often been necessary to modify completed verifications to accommodate changed assumptions or requirements, and people other than the original developer have often needed to understand, review, build on, modify, or extract part of an intricate verification. In this paper, we outline the verifications performed, present the lessons learned, and describe some of the design decisions taken in PVS to better support these large, difficult, iterative, and collaborative verifications

[12] Sam Owre, John Rushby, Natarajan Shankar, and Friedrich von Henke. Formal verification for fault-tolerant architectures: Some lessons learned. In J. C. P. Woodcock and P. G. Larsen, editors,

Introduction, volume 121 and volume 123 of Studies in Logic and the Foundations of Mathematics. North-Holland, Amsterdam, Holland, 1988. In two volumes. Reference