13 research outputs found
Verticillium longisporum induzierte Genexpression in Arabidopsis thaliana
Verticillium longisporum ist ein bodenbürtiger Pilz,
welcher die Rapswelke hervorrufen kann. Das Pathogen
dringt über die Wurzeln in die Wirtspflanze ein und
wächst in Form eines Myzels im Xylem. Im späteren
Infektionsverlauf befällt es schließlich die übrigen
Gewebe der Pflanze. Um die Reaktionen infizierter
Pflanzen zu charakterisieren wurde die Modelpflanze
Arabidopsis thaliana gewählt. Es wurden
Transkriptomprofile der Rosetten fünf und 18 Tage nach
Infektion (dpi) aufgenommen. Fünf dpi zeigt A. thaliana
noch keine Symptome. Es werden jedoch eine Reihe von
Genen mit Bezug zur Jasmonsäure (JA) biosynthese oder
singnaltransduktion induziert. Durch die Infektion von
Pflanzen mit einer Mutation im JA-Biosyntheseweg wurde
der Einfluss dieser Gene auf die Symptomentwicklung bei
Arabidopsis untersucht. Die Reaktion der Pflanze 5 dpi
beeinflusste den Pathophenotyp und die Expression der
Verticillium longisporum induzierten Gene (VliGs) 18
Tage nach Infektion nicht. Mehr als ein Viertel (5 dpi)
bzw. ein Drittel (18 dpi) der VliGs codiert für
apoplastisch lokalisierte Gene, welche an
Zellwandmodifikationen und potentiellen
Abwehrreaktionen beteiligt sind. Ihre Induktion war
Pilzisolat abhängig. Die identifizierten VliGs stellen
ein nützliches Mittel zur Aufklärung de
Influence of <i>Verticillium longisporum</i> VL43 infection on protein content of whole leaf extracts, in apoplastic washing fluid and on the number of protein spots after 2-D electrophoresis.
<p>Plants were analyzed 25 days post infection. Protein spots were determined by Proteomweaver software in silver stained gels (cf. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031435#pone-0031435-g001" target="_blank">Fig. 1</a>). Data indicate means (N ± SE).</p
Fungal DNA, stunting of the rosette, and relative transcript abundance of genes encoding apoplastic proteins from <i>Arabidopsis thaliana</i> that were either induced or repressed by treatment with <i>Verticillium longisporum</i> (VL43).
<p>(A) Typical time course of stunting white symbols) and VL43 DNA (black symbols) in Arabidopsis rosettes (n≥4 biological replicates ± SE, except 10 dpi, where pooled samples were analyzed due limited material), T = traces of VL43 DNA detected. Stunting was determined as projected rosette area of VL infected plants/projected rosette area of mock inoculated plants. (B) Transcript levels of <i>CILLP</i>, <i>SCPL20</i>, <i>AGAL2</i> and <i>GLP3</i> and (C) transcript levels of the peroxidases <i>PRX52</i> (right axis), <i>PRX34</i>, <i>P37</i> and <i>PA2</i> (left axis). Transcript levels were normalized to actin and expressed as transcript abundance in VL-infected plants/transcript abundance in mock-inoculated plants (n≥6 biological replicates ± SE). *indicate significant differences between mock and VL-infected plants.</p
<i>Verticillium longisporum</i> VL43 induced disease symptoms in <i>Arabidopsis thaliana</i>.
<p>Measurements were taken 24 or 25 days post inoculation on the indicated number (n) of plants. Data indicate means (± SE). Values of <i>p</i>≤0.05 indicate significant differences between mock-inoculated and <i>Verticillium longisporum</i> (VL)-infected plants.</p
Typical 2-D gels showing <i>Arabidopsis thaliana</i> apoplastic leaf proteins after mock (A) or <i>Verticillium longisporum</i>
<p><b>(VL43) infection (B).</b> Proteins were extracted 25 dpi and 80 µg were loaded for separation. Gels were stained with silver nitrate. Numbers in the gels indicate protein spots analyzed by ESI-LC/MS from preparative Coomassie-stained gels.</p
Fourier transform infrared spectra of cell walls of mock- and <i>Verticillium longisporum</i> VL43-infected <i>Arabidopsis thaliana</i> plants (A) and the difference spectrum (B).
<p>The difference spectrum was generated by subtracting the mean spectrum of cell walls from mock infected plants from that of VL-infected plants. Spectra were recorded with a resolution of 4 nm and are shown in the range from 800–1800 cm<sup>−1</sup>; data were baseline corrected and vector normalized. Mean spectra were calculated (n = 8 per treatment) and subtracted.</p
Purity of apoplastic washing fluid (AWF) from leaves of mock- and <i>Verticillium longisporum</i> VL43-infected leaves.
<p>Plants were harvested 25 days post infection and foliage of 10 plants was pooled to obtain AWF. Five independent pools were analyzed per treatment. Data indicate means (± SE) for malate dehydrogenase activity, which is a symplastic marker.</p
Infection markers identified in leaves of the apoplast of <i>Arabidopsis thaliana</i> infected with <i>Verticillium longisporum</i> VL43.
<p>AWF was obtained from <i>A. thaliana</i> leaves 21 days post infection and analyzed by UPLC-TOF-MS. Data are means of 3 biological and 3 technical replicates. SI = Signal intensity, Mode of identification refers to A) Exact mass measurement, B) retention time (RT) comparison to authentic standards, C) UV/VIS spectrum, and D) MS/MS fragmentation. <i>P</i>-values were calculated by Students t-test.</p
<em>Verticillium longisporum</em> Infection Affects the Leaf Apoplastic Proteome, Metabolome, and Cell Wall Properties in <em>Arabidopsis thaliana</em>
<div><p><em>Verticillium longisporum</em> (VL) is one of the most devastating diseases in important oil crops from the family of Brassicaceae. The fungus resides for much time of its life cycle in the extracellular fluid of the vascular system, where it cannot be controlled by conventional fungicides. To obtain insights into the biology of VL-plant interaction in the apoplast, the secretome consisting of the extracellular proteome and metabolome as well as cell wall properties were studied in the model Brassicaceae, <em>Arabidopsis thaliana</em>. VL infection resulted in increased production of cell wall material with an altered composition of carbohydrate polymers and increased lignification. The abundance of several hundred soluble metabolites changed in the apoplast of VL-infected plants including signalling and defence compounds such as glycosides of salicylic acid, lignans and dihydroxybenzoic acid as well as oxylipins. The extracellular proteome of healthy leaves was enriched in antifungal proteins. VL caused specific increases in six apoplast proteins (three peroxidases PRX52, PRX34, P37, serine carboxypeptidase SCPL20, α-galactosidase AGAL2 and a germin-like protein GLP3), which have functions in defence and cell wall modification. The abundance of a lectin-like, chitin-inducible protein (CILLP) was reduced. Since the transcript levels of most of the induced proteins were not elevated until late infection time points (>20 dpi), whereas those of <em>CILLP</em> and <em>GLP3</em> were reduced at earlier time points, our results may suggest that VL enhances its virulence by rapid down-regulation and delay of induction of plant defence genes.</p> </div
Proteins significantly affected in the apoplast of <i>Verticillilum longisporum</i> VL43-infected compared with that of mock-infected <i>Arabidopsis thaliana</i> leaves.
<p>*Factor: protein abundance in samples of VL-treated plants/protein abundance in mock-inoculated plants,</p><p>**92% homology to PRX At437520.</p><p>Only those spots were analyzed that were reproducibly observed in two independent experiments. In each experiment three biological replicates were analyzed. For further details, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031435#pone-0031435-t004" target="_blank">Table 4</a>. Spot identification numbers refer to those shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031435#pone-0031435-g001" target="_blank">Figure 1</a>. Peptides are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031435#pone.0031435.s003" target="_blank">Table S1</a>.</p