10 research outputs found
Raw labeled reads from faecal samples of Calidris alpina, C. alba and Plectrophenax nivalis
Collected while handling the birds or from the field while observing the birds. DNA extracted from individual pellets using Zymo Research Faecal Mini Kit. Sequenced using Ion Torrent PGM. FASTQ file header stands for:
@READNUMBER;barcodelabel=SAMPLEID_COLLECTIONLOCALITY_DAY-MONTH-YEAR_BIRDSPECIES_BIRDAGE
The final readmap
This readmap contains the final read counts for each sample. The difference to raw readmap is that some OTU's in this version have been clumped together. The information in this sheet was used as presense/absence data in the study
Command pipeline used for bioinformatics
The file contains all the commandds used in this study to generate the final data. The bioinformatics was carried out at servers on CSC - IT Center for SCience in Finland
Raw labeled reads from samples of Pardosa glacialis, Xysticus deichmanni, X. labradorensis, Erigone arctica, and Emblyna borealis
Collected from the field. DNA extracted from half individuals using Qiagen Animla Tissue Kit. Sequenced using Ion Torrent PGM. FASTQ file header stands for: @READNUMBER;barcodelabel=SAMPLEID. SampleID: Xd
OTU sequences for Spider data2
Clustered to OTUs using USEARCH algorithm with default (97%) similarity. This way there is a little bit of oversplitting, and OTUs belonging to same biological species are clustered subsequently
Technical data sheet for IonTorrent PGM runs
The data generated by Ion Torrent PGM for each run in the study
Readmap linking trimmed reads and samples
The readmap shows read count for each sample. The readmap is constructed using USEARCH software
Q20 reads from Spider samples for Spider data2
Collected from the field. DNA extracted from half individuals. Sequenced using 454 pyrosequencing
Command pipeline used for bioinformatics for Spider data2
The file contains all the commands used in this study to generate the final data. The bioinformatics was carried out at servers on CSC - IT Center for SCience in Finland
Readmap linking trimmed reads and samples for Spider data2
The readmap shows read count for each sample. The readmap is constructed using USEARCH software