Additional file 1: of Long-term Staphylococcus aureus decolonization in patients on home parenteral nutrition: study protocol for a randomized multicenter trial

Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) Checklist. (DOC 122 kb

Additional file 3: of Reduced rate of intensive care unit acquired gram-negative bacilli after removal of sinks and introduction of ‘water-free’ patient care

Colonization with Gram-negative bacilli. (DOCX 28 kb

Additional file 1: of Challenges in detection and treatment of multidrug resistant tuberculosis patients in Vietnam

Definitions and Interview guide (DOCX 28 kb

Presentation signs and symptoms of 15 measles acute encephalitis patients.

<p>+ and – symbols indicate present and absent, respectively.</p>§<p>Other symptoms: 006 and 008 had lymphadenopathy; 011 had papilloedema; 031 had an abnormal abdominal exam.</p>†<p>These patients developed encephalitis signs after admission and CSF was collected at that time.</p

Lymphocyte subset counts in AME patients versus controls.

<p>Results for CD8 (A), CD4 (B) and CD19 (C) counts during early illness (day 5–13) are shown for 13 controls (blue circles) and 6 AME patients (red filled circles). Results are also shown for AME patients in late illness (n = 8) and following recovery (n = 6) with lines connecting multiple time points for the same patient. Horizontal lines represent the lower +/− upper limits of the normal range.</p

Relationship between age and serum concentration and avidity of measles-reactive IgG in AME patients and controls.

<p>Results are shown for 15 AME patients (top panels) and 13 controls (bottom panels). Results are categorized as being tested before (○) or from day 7 since fever onset (•) because titers increased from day 7. Results for tests done before and after day 7 since fever for two AME patients are joined by a line. One patient tested more than 19 days after fever onset is indicated (*). Sera that were tested after administration of IVIG are also indicated (#). Vertical lines differentiate patients born before and after measles vaccine was widely available in Vietnam. Horizontal lines in panel B indicate avidity cut-offs for identifying secondary (upper) and primary (lower) type antibody responses with intermediate levels being indeterminate.</p

Acute Measles Encephalitis in Partially Vaccinated Adults

<div><p>Background</p><p>The pathogenesis of acute measles encephalitis (AME) is poorly understood. Treatment with immune-modulators is based on theories that post-infectious autoimmune responses cause demyelination. The clinical course and immunological parameters of AME were examined during an outbreak in Vietnam.</p><p>Methods and Findings</p><p>Fifteen measles IgM-positive patients with confusion or Glasgow Coma Scale (GCS) score below 13, and thirteen with uncomplicated measles were enrolled from 2008–2010. Standardized clinical exams were performed and blood collected for lymphocyte and measles- and auto-antibody analysis. The median age of AME patients was 21 years, similar to controls. Eleven reported receiving measles vaccination when aged one year. Confusion developed a median of 4 days after rash. Six patients had GCS <8 and four required mechanical ventilation. CSF showed pleocytosis (64%) and proteinorrhachia (71%) but measles virus RNA was not detected. MRI revealed bilateral lesions in the cerebellum and brain stem in some patients. Most received dexamethasone +/− IVIG within 4 days of admission but symptoms persisted for ≥3 weeks in five. The concentration of voltage gated calcium channel-complex-reactive antibodies was 900 pM in one patient, and declined to 609 pM ∼ 3 months later. Measles-reactive IgG antibody avidity was high in AME patients born after vaccine coverage exceeded 50% (∼ 25 years earlier). AME patients had low CD4 (218/µl, p = 0.029) and CD8 (200/µl, p = 0.012) T-cell counts compared to controls.</p><p>Conclusion</p><p>Young adults presenting with AME in Vietnam reported a history of one prior measles immunization, and those aged <25 years had high measles-reactive IgG avidity indicative of prior vaccination. This suggests that one-dose measles immunization is not sufficient to prevent AME in young adults and reinforces the importance of maintaining high coverage with a two-dose measles immunization schedule. Treatment with corticosteroids and IVIG is common practice, and should be assessed in randomized clinical trials.</p></div

Timing of confusion in relation to rash and treatment with steroids and IVIG for 15 AME patients.

<p>Rash (purple); confusion (green); steroids (orange); IVIG (dark green).</p

Sensitivity of MODS, LJ culture, and smear against clinical reference standard.

<p>The sensitivities of MODS, LJ culture, and smear were calculated against the clinical reference standard. Sensitivities were calculated by all samples (some patients provided more than one sample), by patient, and by sample type. Exact binomial 95% confidence intervals were calculated.</p>*<p>Comparison of sensitivities using Exact McNemar's Test: MODS vs LJ and MODS vs Smear.</p

Demographic characteristics of patients.

<p>Summary measure for categorical data is n (%). * P is used to compare across all three groups (confirmed, probable, and unlikely). For continuous variables a Kruskal-Wallis rank sum test was used. When P<0.05 Fisher's Exact test was used to compare confirmed with probable (P1), probable with unlikely (P2), and confirmed with unlikely (P3). For continuous variables a Wilcoxon rank sum test was used for P1, P2, and P3 calculations.</p