Carpenter Ants

(Camponotus) Reference work entry</p

Study ponds assigned according to the results of the k-means clustering.

<p>Ponds are plotted in the first two principal components using 16 abiotic habitat characteristics, explaining 40.53 % of data variance. Ponds with emigrating <i>Rana temporaria</i> metamorphs are indicated by filled, non-emigration ponds by open triangles. Loadings of the components are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089982#pone.0089982.s003" target="_blank">Table S3</a>.</p

Summary of metamorphing <i>Rana temporaria</i> juveniles and predator- and tadpole-density in the respective ponds in 2007.

<p>Survival rate was based on an average of 1117 eggs per clutch. For metamorphic traits, mean, coefficient of variance (CV), as well as minimum and maximum size and mass of individuals for each pond is given. For predator- and tadpole-density, mean and SD of all samplings, number of sampling events and total number of box samplings summed up for realized samplings are given (see methods for detailed description). For pond abbreviations, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089982#pone.0089982-Grzinger1" target="_blank">[31]</a>.</p><p>cl.  = clutches; meas.  = measured, devel.time = development time, samp.  = samplings: events/total samplings.</p

Pond characteristics and metamorphic traits.

<p>Visualization of environmental parameters in relation to <i>Rana temporaria</i> metamorphic mass (median) and development time (median). Values are colour-coded, warm colours indicating higher values. Ponds (bottom) are ordered according to their values for the respective trait, the values for the respective environmental parameter are given in the column above. There is no correlation between traits and environmental parameter visible (rows).</p

ANOVA for mass, size, and development time of metamorphosing <i>Rana temporaria</i>.

<p>Given are the data for five ponds and three years. df  =  degrees of freedom.</p

<i>Rana temporaria</i> metamorphs from two different ponds in the study area.

<p>Although being in the same developmental stage and emigrating at the same date, the differences in size, proportion and vitality are obvious. Individual left: 10.2 mm, 0.09 g, right: 17.2 mm, 0.56 g, 23 July 2009.</p

Metamorphic mass <i>of Rana temporaria</i> for five ponds in the years 2007, 2009 and 2010.

<p>For values of size and development time, see Table S7.</p

Most important parameters (see Table S1) describing the pattern of <i>Rana temporaria</i> metamorphosis mass in study ponds.

<p>Emigration mass in study ponds (n = 10, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089982#pone-0089982-g004" target="_blank">Figure 4</a>) is described according to: log(mass) ∼x × development-time +z × development-time². Given are the parameter combinations for the coefficients of slope (x) and bend (z) of the curve in the linear mixed model, which showed the lowest AICc (Akaike Information Criterion for finite sample sizes) after model selection (i.e. ΔAICc = AICc_i−AICc_min, <200). ‘Pond’ was used as random factor. Akaike mass  =  normalized relative likelihood of the model given the data.</p

PCR amplification of full length <i>hymenoptaecin</i> gene and cDNA.

<p>The PCR-products from gDNA (lane 1) and cDNA (lane 2) were separated on a 1.2% agarose gel alongside molecular size markers (lane M, GeneRuler 1 kb DNA Ladder, Fermentas) and analyzed with EtBr staining. The major bands correspond to the full length <i>hymenoptaecin</i> gene- (3356 bp lane 1) and cDNA-product (2536 bp, lane 2). The minor bands are technical artefacts with variable repeat numbers caused by the tandem repeats.</p

data_Rana_temporaria_mating_speed

Contains raw data of all trials of the mating speed experiment (n=44). Given are snout-vent-length in mm for female, small male and large male per trial; wich male was faster in grabbing the female first (Male_ampl); the size difference of female and non-amplexed male in mm; the size difference of female and male in amplexus in mm