Ibactericidal and bacteriostatic in vitro effects of teucrium chamaedrys hydroalcoholic extract on two bacterial causative agents of tooth decay

Tooth decay is a prevalent dental disease and Streptococcus mutans and Lactobacillus rhamnosis are the most important bacterial causes of tooth decay. Because of the increased antibiotic resistance of bacteria and the side effects of antibiotics, this study was conducted to investigate the antibacterial effect of hydroalcoholic Teucrium Chamaedrys extraction S.mutans and L.rhamnosis. In this experimental-laboratory study, T.chamaedrys was extracted by maceration, and the standard strains of lyophilized S.mutans and L.rhamnosis were provided from Iranian Research Organization for Science and Technology for investigation of T.chamaedrys effect on their growth. The antibacterial effect of hydroalcoholic T.chamaedrys was investigated on S.mutans and L.rhamnosis and then minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The MIC of T. chamaedrys was obtained 128 and 32 μg/L against S.mutans and L.rhamnosis, respectively. The MBC of T.chamaedrys was obtained 256 and 64 μg/L against S.mutans and L.rhamnosis, respectively. T.chamaedrys bactericidal and bacteriostatic effects on L.rhamnosis and S.mutans, two bacterial causative agents of dental cavity show that it might be used for prevention of dental cavity due to these patogens

Identification of a Novel Mutation in an Iranian Family with 17-β Hydroxysteroid Dehydrogenase Type 3 Deficiency; A case series

Background: To present the clinical and genetic features of a male ambiguity due to 17beta-hydroxysteroid dehydrogenase 3 (17B-HSD3) deficiency. Case presentation: The proposita was an 11-year-old girl and the first child of a consanguineous family. The external genitalia were completely female and had a short vaginal pouch. She had palpable gonads in her inguinal area and underwent bilateral gonadectomy at the age of two. At age 10, she was referred to our clinic for more evaluation. In pelvic sonography, uterine and ovarian were not seen. Her karyotype was 46, XY and her LH and FSH levels were elevated, and three of the patient's aunts and one of the mother's aunts had similar signs. Conclusion: We identified a novel homozygous missense variation (c.731T>A, p. Ile244Lys) in HSD17B3 gene. This alteration changes Isoleucine to Lysine in exon 10. Keywords: 17-β-HSD3, Virilization, 46, XY, Ira

Bactericidal and Bacteriostatic effect of sesame oil, olive oil and their synergism on Escherichia coli in vitro

Background and aims: Escherichia coli is one of the important bacteria in nosocomial infections and its resistance to a wide range of antibiotics caused many problems for doctors and patients. So, it was attempted to find new substitution for these antibiotics. The aim of the present study was to determine the antimicrobial effect of olive oil, sesame oil and their synergism on E. coli. Methods: In this study, olive oil and sesame oil were extracted by cold press assay and standard strain of lyophilized E. coli ATCC 25922 was prepared from the Iranian Research Organization for Science and Technology. Olive oil, sesame oil and their synergism, with the same proportion, in concentrations of 2 mg/ml to 1024 mg/ml were made individually and their MBC and MIC amounts were determined by broth micro dilution. Results: This study declared MIC and MBC for olive oil; 16 mg/ml and 64 mg/ml, for sesame oil and their mixture; 64 mg/ml and 265mg/ml, respectively. Conclusion: The study showed that both oils and their synergism inhibits the growth of bacteria and the antibacterial properties of sesame oil is approximately equal to the mixture of olive oil and sesame oil and although the oils have good antibacterial effect, there was no synergetic effect in these oils

Down-regulation of miR-135b in colon adenocarcinoma induced by a TGF-β receptor I kinase inhibitor (SD-208).

OBJECTIVES Transforming growth factor-β (TGF-β) is involved in colorectal cancer (CRC). The SD-208 acts as an anti-cancer agent in different malignancies via TGF-β signaling. This work aims to show the effect of manipulation of TGF-β signaling on some miRNAs implicated in CRC. MATERIALS AND METHODS We investigated the effects of SD-208 on SW-48, a colon adenocarcinoma cell line. The cell line was treated with 0.5, 1 and 2 μM concentrations of SD-208. Then, the xenograft model of colon cancer was established by subcutaneous inoculation of SW-48 cell line into the nude mice. The animals were treated with SD-208 for three weeks. A quantitative real-time PCR was carried out for expression level analysis of selected oncogenic (miR-21, 31, 20a and 135b) and suppressor-miRNAs (let7-g, miR-133b, 145 and 200c). Data were analyzed using the 2-∆∆CT method through student's t-test via the GraphPad Prism software. RESULTS Our results revealed that SD-208 could significantly down-regulate the expression of one key onco-miRNA, miR-135b, in either SW-48 colon cells (P=0.006) or tumors orthotopically implanted in nude mice (P=0.018). Our in silico study also predicted that SD-208 could modulate the expression of potential downstream tumor suppressor targets of the miR135b. CONCLUSION Our data provide novel evidence that anticancer effects of SD-208 (and likely other TGF-β inhibitors) may be owing to their ability to regulate miRNAs expression