2,821 research outputs found
Blood Digestion in the Mosquito, Anopheles stephensi Liston (Diptera: Culicidae): Activity and Distribution of Trypsin, Aminopeptidase, and α-Glucosidase in the Midgut
The activities of trypsin, aminopeptidase, and α-glucosidase were studied in the whole midgut, anterior and posterior midgut, and posterior midgut lumen and epithelium of the mosquito Anopheles stephensi Liston. Trypsin activity was restricted entirely to the posterior midgut lumen. No trypsin activity was found before the blood meal, but activity increased continuously up to 30 h after feeding, and subsequently returned to baseline levels by 60 h. Aminopeptidase was active in anterior and posterior midgut regions before and after feeding. In whole midguts, activity rose from a baseline of ≍3 enzyme units (EU) per midgut to a maximum of 12 EU at 30 h after the blood meal, subsequently falling to baseline levels by 60 h. A similar cycle of activity was observed in the posterior midgut and posterior midgut lumen, whereas aminopeptidase in the posterior midgut epithelium decreased in activity during digestion. Aminopeptidase in the anterior midgut was maintained at a constant low level, showing no significant variation with time after feeding, α-glucosidase was active in anterior and posterior midguts before and at all times after feeding. In whole midgut homogenates, α-glucosidase activity increased slowly up to 18 h after the blood meal, then rose rapidly to a maximum at 30 h after the blood meal, whereas the subsequent decline in activity was less predictable. All posterior midgut activity was restricted to the posterior midgut lumen. Depending upon the time after feeding, >25% of the total midgut activity of α-glucosidase was located in the anterior midgut. The enzyme distributions are consistent with described structural models for digestion in mosquitoes. After blood meal ingestion, proteases are active only in the posterior midgut. Trypsin is the major primary hydrolytic protease and is secreted into the posterior midgut lumen without activation in the posterior midgut epithelium. Aminopeptidase activity is also luminal in the posterior midgut, but cellular aminopeptidases are required for peptide processing in both anterior and posterior midguts. α-glucosidase activity is elevated in the posterior midgut after feeding in response to the blood meal, whereas activity in the anterior midgut is consistent with a nectarprocessing role for this midgut regio
Coherent optical transfer of Feshbach molecules to a lower vibrational state
Using the technique of stimulated Raman adiabatic passage (STIRAP) we have
coherently transferred ultracold 87Rb2 Feshbach molecules into a more deeply
bound vibrational quantum level. Our measurements indicate a high transfer
efficiency of up to 87%. As the molecules are held in an optical lattice with
not more than a single molecule per lattice site, inelastic collisions between
the molecules are suppressed and we observe long molecular lifetimes of about 1
s. Using STIRAP we have created quantum superpositions of the two molecular
states and tested their coherence interferometrically. These results represent
an important step towards Bose-Einstein condensation (BEC) of molecules in the
vibrational ground state.Comment: 4 pages, 5 figure
Detection of water molecules on the radical transfer pathway of ribonucleotide reductase by 17O electron–nuclear double resonance spectroscopy
The role of water in biological proton-coupled electron transfer (PCET) is emerging as a key for understanding mechanistic details at atomic resolution. Here we demonstrate 17O high-frequency electron–nuclear double resonance (ENDOR) in conjunction with H217O-labeled protein buffer to establish the presence of ordered water molecules at three radical intermediates in an active enzyme complex, the α2β2E. coli ribonucleotide reductase. Our data give unambiguous evidence that all three, individually trapped, intermediates are hyperfine coupled to one water molecule with Tyr-O···17O distances in the range 2.8–3.1 Å. The availability of this structural information will allow for quantitative models of PCET in this prototype enzyme. The results also provide a spectroscopic signature for water H-bonded to a tyrosyl radical
Cruising through molecular bound state manifolds with radio frequency
The emerging field of ultracold molecules with their rich internal structure
is currently attracting a lot of interest. Various methods have been developed
to produce ultracold molecules in pre-set quantum states. For future
experiments it will be important to efficiently transfer these molecules from
their initial quantum state to other quantum states of interest. Optical Raman
schemes are excellent tools for transfer, but can be involved in terms of
equipment, laser stabilization and finding the right transitions. Here we
demonstrate a very general and simple way for transfer of molecules from one
quantum state to a neighboring quantum state with better than 99% efficiency.
The scheme is based on Zeeman tuning the molecular state to avoided level
crossings where radio-frequency transitions can then be carried out. By
repeating this process at different crossings, molecules can be successively
transported through a large manifold of quantum states. As an important
spin-off of our experiments, we demonstrate a high-precision spectroscopy
method for investigating level crossings.Comment: 5 pages, 5 figures, submitted for publicatio
Hyperfine, rotational and Zeeman structure of the lowest vibrational levels of the Rb \tripletex state
We present the results of an experimental and theoretical study of the
electronically excited \tripletex state of Rb molecules. The
vibrational energies are measured for deeply bound states from the bottom up to
using laser spectroscopy of ultracold Rb Feshbach molecules. The
spectrum of each vibrational state is dominated by a 47\,GHz splitting into a
\cog and \clg component caused mainly by a strong second order spin-orbit
interaction. Our spectroscopy fully resolves the rotational, hyperfine, and
Zeeman structure of the spectrum. We are able to describe to first order this
structure using a simplified effective Hamiltonian.Comment: 10 pages, 7 figures, 2 table
Repulsively bound atom pairs: Overview, Simulations and Links
We review the basic physics of repulsively bound atom pairs in an optical
lattice, which were recently observed in the laboratory, including the theory
and the experimental implementation. We also briefly discuss related many-body
numerical simulations, in which time-dependent Density Matrix Renormalisation
Group (DMRG) methods are used to model the many-body physics of a collection of
interacting pairs, and give a comparison of the single-particle quasimomentum
distribution measured in the experiment and results from these simulations. We
then give a short discussion of how these repulsively bound pairs relate to
bound states in some other physical systems.Comment: 7 pages, 3 figures, Proceedings of ICAP-2006 (Innsbruck
O-17 Hyperfine Spectroscopy Reveals Hydration Structure of Nitroxide Radicals in Aqueous Solutions
The hydrationstructureof nitroxideradicalsin aqueoussolutionsis elucidatedby advanced17Ohyperfine(hf) spectroscopywith supportof quantumchemicalcalculationsand MD simulations.A piperidineand a pyrrolidine-basednitroxideradical are comparedand show clear differencesin the preferreddirectionalityof H-bondformation.We demonstratethat thesescenariosare best representedin17O hf spectra,wherein-planecoordinationovers-type H-bondingleads tolittle spin densitytransferon the water oxygenand smallhf couplings,whereasp-type perpendicularcoordinationgeneratesmuch larger hf couplings.Quantitativeanaly-sis of the spectrabased on MD simulationsand DFTpredictedhf parametersis consistentwith a distributionof close solvatingwater molecules,in which direction-ality is influencedby subtle steric effects of the ring andthe methyl group substituents
Evaluation of Tibial Fixation Devices for Quadrupled Hamstring ACL Reconstruction
BACKGROUND
Shortcomings to tibial-side fixation have been reported as causes of failure after anterior cruciate ligament reconstruction. Adjustable-loop suspensory devices have become popular; however, no comparison with hybrid fixation (ie, interference screw and cortical button) exists to our knowledge.
PURPOSE
The purpose of this study was to compare the biomechanical properties of adjustable loop devices (ALDs) in full-tunnel and closed-socket configurations in relation to hybrid fixation. We hypothesized that primary stability of fixation by a tibial ALD will not be inferior to hybrid fixation.
STUDY DESIGN
Controlled laboratory study.
METHODS
Tibial fixation of a quadrupled tendon graft was biomechanically investigated in a porcine tibia-bovine tendon model using 5 techniques (n = 6 specimens each). The tested constructs included hybrid fixation with a cortical fixation button and interference screw (group 1), single cortical fixation with the full-tunnel technique using an open-suture strand button (group 2) or an ALD (group 3), or closed-socket fixation using 2 different types of ALDs (groups 4 and 5). Each specimen was evaluated using a materials testing machine (1000 cycles from 50-250 N and pull to failure). Force at failure, cyclic displacement, stiffness, and ability to pretension the graft during insertion were compared among the groups.
RESULTS
No differences in ultimate load to failure were found between the ALD constructs (groups 3, 4, and 5) and hybrid fixation (group 1). Cyclic displacement was significantly higher in group 2 vs all other groups (P < .001); however, no difference was observed in groups 3, 4, and 5 as compared with group 1. The remaining tension on the construct after fixation was significantly higher in groups 3 and 4 vs groups 1, 2, and 5 (P < .02 for all comparisons), irrespective of whether a full-tunnel or closed-socket approach was used.
CONCLUSION
Tibial anterior cruciate ligament graft fixation with knotless ALDs achieved comparable results with hybrid fixation in the full-tunnel and closed-socket techniques. The retention of graft tension appears to be biomechanically more relevant than tunnel type.
CLINICAL RELEVANCE
The study findings emphasize the importance of the tension at which fixation is performed
Conductance fluctuations in mesoscopic normal-metal/superconductor samples
We study the magnetoconductance fluctuations of mesoscopic
normal-metal/superconductor (NS) samples consisting of a gold-wire in contact
with a niobium film. The magnetic field strength is varied over a wide range,
including values that are larger than the upper critical field B_c2 of niobium.
In agreement with recent theoretical predictions we find that in the NS sample
the rms of the conductance fluctuations (CF) is by a factor of 2.8 +/- 0.4
larger than in the high field regime where the entire system is driven normal
conducting. Further characteristics of the CF are discussed.Comment: 4 pages, REVTEX, 3 eps-figures included. To be published in Phys.
Rev. Lett.. Changes: one misplaced figure correcte
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