Стан і тенденції трансформації православних сект в незалежній Україні

Since the introduction of “soft” ionization techniques, the role of mass spectrometry (MS) in the field of structural biology has increasingly expanded. With the incorporation of volatile buffers as electrospray ionization (ESI) solvents, non-covalent protein complexes could be efficiently transferred to the gas phase for mass analysis. While native MS has not become a technique used for standard characterization of therapeutic proteins in an industrial setting, it is increasingly used to probe the structural heterogeneity of these complex biomolecules. Here, we describe a detailed sample protocol for the analysis of monoclonal antibodies (mAbs) by native MS and highlight some recent applications of native MS in the analysis of intact mAbs and mAb-based therapeutics

Аналіз можливості єдиного визначення «економічної системи», її структури та зв’язків

Next-generation sequencing allows the analysis of genomes, including those representing disease states. However, the causes of most disorders are multifactorial, and systems-level approaches, including the analysis of proteomes, are required for a more comprehensive understanding. The proteome is extremely multifaceted owing to splicing and protein modifications, and this is further amplified by the interconnectivity of proteins into complexes and signalling networks that are highly divergent in time and space. Proteome analysis heavily relies on mass spectrometry (MS). MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis. Here we describe this emerging next generation of proteomics and highlight recent applications

З листування Л. Биковського з Є. Бачинським

У статті надається листування видатного бібліографа, історика Л. Биковського з визначним українським громадським і церковним діячем Є. Бачинським. листи написані в 1947–1952 рр.В статье представлена переписка выдающегося библиографа, историка Л. Быковского с выдающимся украинским общественным и церковным деятелем Е. Бачинским. Письма написаны в 1947-1952 гг.The article consists of correspondence of the outstanding bibliographer, historian L.Bykovskyi with the outstanding Ukrainian public and church figure Ye. Bachynskyi. Letters are written in 1947–1952

Архієпископ Кримський Лука про способи духовного оздоровлення Кримської єпархії

In the past decade, mass-spectrometry-based methods have emerged for the quantitative profiling of dynamic changes in protein phosphorylation, allowing the behavior of thousands of phosphorylation sites to be monitored in a single experiment. However, when one is interested in specific signaling pathways, such shotgun methodologies are not ideal because they lack selectivity and are not cost and time efficient with respect to instrument and data analysis time. Here we evaluate and explore a peptide-centric antibody generated to selectively enrich peptides containing the cAMP-dependent protein kinase (PKA) consensus motif. This targeted phosphoproteomic strategy is used to profile temporal quantitative changes of potential PKA substrates in Jurkat T lymphocytes upon prostaglandin E2 (PGE2) stimulation, which increases intracellular cAMP, activating PKA. Our method combines ultra-high-specificity motif-based immunoaffinity purification with cost-efficient stable isotope dimethyl labeling. We identified 655 phosphopeptides, of which 642 (i.e. 98%) contained the consensus motif [R/K][R/K/X]X[pS/pT]. When our data were compared with a large-scale Jurkat T-lymphocyte phosphoproteomics dataset containing more than 10,500 phosphosites, a minimal overlap of 0.2% was observed. This stresses the need for such targeted analyses when the interest is in a particular kinase. Our data provide a resource of likely substrates of PKA, and potentially some substrates of closely related kinases. Network analysis revealed that about half of the observed substrates have been implicated in cAMP-induced signaling. Still, the other half of the here-identified substrates have been less well characterized, representing a valuable resource for future research

Механізми вдосконалення управління інноваційною діяльністю в умовах економічної нестабільності

Розглядаються особливості глобально-постіндустріального розвитку. Досліджено методичні основи системи моніторингу, прогнозування, планування і забезпечення реалізації інноваційної діяльності та головні засади механізму її функціонування.Peculiarities of the global post-industrial development are reviewed. Methodical fundamentals of the system for monitoring, forecasting, planning and provision of innovation activity implementation and main principles of its functioning mechanisms are reviewed

Спосіб фіксації гістологічних блоків для виготовлення багатоплощинних зрізів мозочка

В статье описывается способ фиксации гистологических блоков для изготовления многоплоскостных срезов мозжечка, который можно также использовать для гистологического изучения других тканей. Дано подробное описание способа. Обоснованы преимущества его применения. Приведены графические изображения.In article the fixation mode of histological block for preparation of multiplane sections of cerebellum is described. The positive characteristics are well-grounded

Elucidating crosstalk mechanisms between phosphorylation and O-GlcNAcylation

Chemical Immunolog

Quantifying positional isomers (QPI) by top-down mass spectrometry

Proteomics has exposed a plethora of posttranslational modifications, but demonstrating functional relevance requires new approaches. Top-down proteomics of intact proteins has the potential to fully characterize protein modifications in terms of amount, site(s), and the order in which they are deposited on the protein; information that so far has been elusive to extract by shotgun proteomics. Data acquisition and analysis of intact multimodified proteins have however been a major challenge, in particular for positional isomers that carry the same number of modifications at different sites. Solutions were previously proposed to extract this information from fragmentation spectra, but these have so far mainly been limited to peptides and have entailed a large degree of manual interpretation. Here, we apply high-resolution Orbitrap fusion top-down analyses in combination with bioinformatics approaches to attempt to characterize multiple modified proteins and quantify positional isomers. Automated covalent fragment ion type definition, detection of mass precision and accuracy, and extensive use of replicate spectra increase sequence coverage and drive down false fragment assignments from 10% to 1.5%. Such improved performance in fragment assignment is key to localize and quantify modifications from fragment spectra. The method is tested by investigating positional isomers of Ubiquitin mixed in known concentrations, which results in quantification of high ratios at very low standard errors of the mean (<5%), as well as with synthetic phosphorylated peptides. Application to multiphosphorylated Bora provides an estimation of the so far unknown stoichiometry of the known set of phosphosites and uncovers new sites from hyperphosphorylated Bora.Chemical Immunolog

Tyrosine phosphorylation profiling in FGF-2 stimulated human embryonic stem cells

10.1371/journal.pone.0017538PLoS ONE63