LiveShift: Mesh-pull live and time-shifted P2P video streaming

The popularity of video sharing over the Internet has increased significantly. High traffic generated by such applications at the source can be better distributed using a peer-to-peer (P2P) overlay. Unlike most P2P systems, LiveShift combines both live and on-demand video streaming while video is transmitted through the peer-to-peer network in a live fashion, all peers participate in distributed storage. This adds the ability to replay time-shifted streams from other peers in a distributed and scalable manner. This paper describes an adaptive fully-distributed mesh-pull protocol that supports the envisioned use case and a set of policies that enable efficient usage of resources, discussing interesting trade-offs encountered. User-focused evaluation results, including both channel switching and time shifting behavior, show that the proposed system provides good quality of experience for most users, in terms of infrequent stalling, low playback lag, and a small proportion of skipped blocks in all the scenarios studied, even in presence of churn

Bioinformatics and molecular modeling in glycobiology

The field of glycobiology is concerned with the study of the structure, properties, and biological functions of the family of biomolecules called carbohydrates. Bioinformatics for glycobiology is a particularly challenging field, because carbohydrates exhibit a high structural diversity and their chains are often branched. Significant improvements in experimental analytical methods over recent years have led to a tremendous increase in the amount of carbohydrate structure data generated. Consequently, the availability of databases and tools to store, retrieve and analyze these data in an efficient way is of fundamental importance to progress in glycobiology. In this review, the various graphical representations and sequence formats of carbohydrates are introduced, and an overview of newly developed databases, the latest developments in sequence alignment and data mining, and tools to support experimental glycan analysis are presented. Finally, the field of structural glycoinformatics and molecular modeling of carbohydrates, glycoproteins, and protein–carbohydrate interaction are reviewed

Fragility in the 14q21q translocation region

Aphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicated the absence of beta-satellite signal at the translocation region. Per-individual analysis of metaphases from APC-treated peripheral blood lymphocyte cultures identified significantly nonrandom chromosomal breakage at the translocation region in all three individuals examined. The APC-inducible fragility at the 14q21q translocation region suggests that this rearrangement was the result of chromosomal mutation at fragile site(s) in the progenitor chromosomes, or that this fragility was the result of the fusion of nonfragile progenitor chromosomes