Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity

Genomic maps of chromatin modifications have provided evidence for the partitioning of genomes into domains of distinct chromatin states, which assist coordinated gene regulation. The maintenance of chromatin domain integrity can require the setting of boundaries. The HS4 insulator element marks the 3′ boundary of a heterochromatin region located upstream of the chicken β-globin gene cluster. Here we show that HS4 recruits the E3 ligase RNF20/BRE1A to mediate H2B mono-ubiquitination (H2Bub1) at this insulator. Knockdown experiments show that RNF20 is required for H2Bub1 and processive H3K4 methylation. Depletion of RNF20 results in a collapse of the active histone modification signature at the HS4 chromatin boundary, where H2Bub1, H3K4 methylation, and hyperacetylation of H3, H4, and H2A.Z are rapidly lost. A remarkably similar set of events occurs at the HSA/HSB regulatory elements of the FOLR1 gene, which mark the 5′ boundary of the same heterochromatin region. We find that persistent H2Bub1 at the HSA/HSB and HS4 elements is required for chromatin boundary integrity. The loss of boundary function leads to the sequential spreading of H3K9me2, H3K9me3, and H4K20me3 over the entire 50 kb FOLR1 and β-globin region and silencing of FOLR1 expression. These findings show that the HSA/HSB and HS4 boundary elements direct a cascade of active histone modifications that defend the FOLR1 and β-globin gene loci from the pervasive encroachment of an adjacent heterochromatin domain. We propose that many gene loci employ H2Bub1-dependent boundaries to prevent heterochromatin spreading

Thermionic reactor power system: Effects of radiation on integration with Manned Space Station

The application of a thermionic reactor power system to the modular space station is described. The nominal net power is 40 kWe, with the power system designed to be applicable over the power range from 25 to 60 kWe. The power system is designed to be launched by the space shuttle. Radiation protection is provided by LiH neutron shielding and W gamma shielding in a shaped 4 pion configuration, i.e., the reactor is shielded on all sides but not to equal extent. Isodose contours are presented for the region around the modular space station. Levels and spectral distribution of radiation are given for later evaluation of effects on space station experiments. Parametric data on the effects of separation distance on power system mass are presented

The control of a nuclear reactor using helium- 3 gas control elements

Control system for water moderated reactor using helium-3 ga

Optimal CDMA signature sequences, inverse eigenvalue problems and alternating minimization

This paper describes the matrix-theoretic ideas known as Welch-bound-equality sequences or unit-norm tight frames that are used to alternate minimizing the total squared correlation. This paper shows the construction of an optimal signature sequences for the synchronous code-division multiple-access (S-CDMA) channel in the presence of white noise and uniform received powers to solve inverse eigenvalue problems that maximize the sum capacity of the S-CDMA channel

Finite-step algorithms for constructing optimal CDMA signature sequences

A description of optimal sequences for direct-spread code-division multiple access (DS-CDMA) is a byproduct of recent characterizations of the sum capacity. This paper restates the sequence design problem as an inverse singular value problem and shows that the problem can be solved with finite-step algorithms from matrix theory. It proposes a new one-sided algorithm that is numerically stable and faster than previous methods

Optimal CDMA signatures: a finite-step approach

A description of optimal sequences for direct-sequence code division multiple access is a byproduct of recent characterizations of the sum capacity. The paper restates the sequence design problem as an inverse singular value problem and shows that it can be solved with finite-step algorithms from matrix analysis. Relevant algorithms are reviewed and a new one-sided construction is proposed that obtains the sequences directly instead of computing the Gram matrix of the optimal signatures

Generalized Finite Algorithms for Constructing Hermitian Matrices with Prescribed Diagonal and Spectrum

In this paper, we present new algorithms that can replace the diagonal entries of a Hermitian matrix by any set of diagonal entries that majorize the original set without altering the eigenvalues of the matrix. They perform this feat by applying a sequence of (N-1) or fewer plane rotations, where N is the dimension of the matrix. Both the Bendel-Mickey and the Chan-Li algorithms are special cases of the proposed procedures. Using the fact that a positive semidefinite matrix can always be factored as \mtx{X^\adj X}, we also provide more efficient versions of the algorithms that can directly construct factors with specified singular values and column norms. We conclude with some open problems related to the construction of Hermitian matrices with joint diagonal and spectral properties