Galaxy Morphology from NICMOS Parallel Imaging

We present high resolution NICMOS images of random fields obtained in parallel to other HST observations. We present galaxy number counts reaching H=24. The H-band galaxy counts show good agreement with the deepest I- and K-band counts obtained from ground-based data. We present the distribution of galaxies with morphological type to H<23. We find relatively fewer irregular galaxies compared to an I-band sample from the Hubble Deep Field, which we attribute to their blue color, rather than to morphological K-corrections. We conclude that the irregulars are intrinsically faint blue galaxies at z<1.Comment: 13 pages, including 4 figures. Accepted for publication in ApJ Letter

Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways

Developing new bioprocesses to produce chemicals and fuels with reduced production costs will greatly facilitate the replacement of fossil-based raw materials. In most fermentation bioprocesses, the feedstock usually represents the highest cost, which becomes the target for cost reduction. Additionally, the biorefinery concept advocates revenue growth from the production of several compounds using the same feedstock. Taken together, the production of bio commodities from low-cost gas streams containing CO, CO2, and H2, obtained from the gasification of any carbon-containing waste streams or off-gases from heavy industry (steel mills, processing plants, or refineries), embodies an opportunity for affordable and renewable chemical production. To achieve this, by studying non-model autotrophic acetogens, current limitations concerning low growth rates, toxicity by gas streams, and low productivity may be overcome. The Acetobacterium wieringae strain JM is a novel autotrophic acetogen that is capable of producing acetate and ethanol. It exhibits faster growth rates on various gaseous compounds, including carbon monoxide, compared to other Acetobacterium species, making it potentially useful for industrial applications. The species A. wieringae has not been genetically modified, therefore developing a genetic engineering method is important for expanding its product portfolio from gas fermentation and overall improving the characteristics of this acetogen for industrial demands.The involved research was fnancially supported by the Portuguese Foundation for Science and Technology (FCT): POCI-01-0145-FEDER-031377; strategic funding of UIDB/04469/2020 unit; BioTecNorte operation (NORTE-01-0145- FEDER-000004); FCT doctoral grant PD/BD/150583/2020info:eu-repo/semantics/publishedVersio

Extremely metal-poor Lyman limit system at z = 2.917 toward the quasar HE 0940-1050

We report on detailed Monte Carlo inversion analysis of the Lyman limit system observed at z = 2.917 in the VLT/UVES spectrum of the quasar HE 0940-1050. Metal absorption lines of carbon and silicon in three ionization stages and numerous atomic hydrogen lines have been analyzed simultaneously. It is found that in order to match the observations, the shape of the ultraviolet background ionizing spectrum of Haardt & Madau (1996) should be modified: a spectrum with a higher intensity of the emission feature at 3 Ryd is required. It is also found that synthetic galactic spectra (or different mixtures of them with power law spectra) cannot reproduce the observations, indicating that the stellar contribution to the ionizing background is negligible at z ~= 3. For the first time a very low carbon abundance of [C/H] = -2.93+/-0.13 and the abundance ratio [Si/C] = 0.35+/-0.15 are directly measured in the Lyman limit system with N(H I) = 3.2 10^{17} cm^{-2}. If the absorber at z = 2.917 provides an example of a pristine gas enriched by the nucleosynthetic products of early generations of stars, then the measured value of [Si/C] seems to indicate that the initial mass functions for these stellar populations are constrained to intermediate masses, M_up <= 25M_solar.Comment: 7 pages, 4 figures, A&A in pres

A Candidate Substellar Companion to HR 7329

We present the discovery of a candidate substellar companion from a survey of nearby, young stars with the NICMOS coronagraph on the Hubble Space Telescope. The H ~ 12 mag object was discovered approximately 4" from the young A0V star HR 7329. Using follow-up spectroscopy from STIS, we derive a spectral type between M7V and M8V with an effective temperature of ~ 2600 K. We estimate that the probability of a chance alignment with a foreground dwarf star of this nature is ~ 10^(-8) and therefore suggest the object (HR 7329B) is physically associated with HR 7329 with a projected separation of 200 AU. Current brown dwarf cooling models indicate a mass of less than 50 Jupiter masses for HR 7329B based on age estimates of < 30 Myr for HR7329A.Comment: 8 pages LATEX, 5 ps figures, accepted for Ap

Emission Line Galaxies in the STIS Parallel Survey I: Observations and Data Analysis

In the first three years of operation STIS obtained slitless spectra of approximately 2500 fields in parallel to prime HST observations as part of the STIS Parallel Survey (SPS). The archive contains almost 300 fields at high galactic latitude (|b|>30) with spectroscopic exposure times greater than 3000 seconds. This sample contains 220 fields (excluding special regions and requiring a consistent grating angle) observed between 6 June 1997 and 21 September 2000, with a total survey area of about 160 square arcminutes. At this depth, the SPS detects an average of one emission line galaxy per three fields. We present the analysis of these data, and the identification of 131 low to intermediate redshift galaxies detected by optical emission lines. The sample contains 78 objects with emission lines that we infer to be redshifted [OII]3727 emission at 0.43<z<1.7. The comoving number density of these objects is comparable to that of H-alpha emitting galaxies in the NICMOS parallel observations. One quasar and three probable Seyfert galaxies are detected. Many of the emission-line objects show morphologies suggestive of mergers or interactions. The reduced data are available upon request from the authors.Comment: 58 preprint pages, including 26 figures; accepted for publication in ApJ

Optical Structure and Proper-Motion Age of the Oxygen-rich Supernova Remnant 1E 0102-7219 in the Small Magellanic Cloud

We present new optical emission-line images of the young SNR 1E 0102-7219 (E0102) in the SMC obtained with the HST Advanced Camera for Surveys (ACS). E0102 is a member of the oxygen-rich class of SNRs showing strong oxygen, neon , and other metal-line emissions in its optical and X-ray spectra, and an absence of H and He. The progenitor of E0102 may have been a Wolf-Rayet star that underwent considerable mass loss prior to exploding as a Type Ib/c or IIL/b SN. The ejecta in this SNR are fast-moving (V > 1000 km/s) and emit as they are compressed and heated in the reverse shock. In 2003, we obtained optical [O III], H-alpha, and continuum images with the ACS Wide Field Camera. The [O III] image captures the full velocity range of the ejecta, and shows considerable high-velocity emission projected in the middle of the SNR that was Doppler-shifted out of the narrow F502N bandpass of a previous Wide Field and Planetary Camera 2 image from 1995. Using these two epochs separated by ~8.5 years, we measure the transverse expansion of the ejecta around the outer rim in this SNR for the first time at visible wavelengths. From proper-motion measurements of 12 ejecta filaments, we estimate a mean expansion velocity for the bright ejecta of ~2000 km/s and an inferred kinematic age for the SNR of \~2050 +/- 600 years. The age we derive from HST data is about twice that inferred by Hughes et al.(2000) from X-ray data, though our 1-sigma error bars overlap. Our proper-motion age is consistent with an independent optical kinematic age derived by Eriksen et al.(2003) using spatially resolved [O III] radial-velocity data. We derive an expansion center that lies very close to X-ray and radio hotspots, which could indicate the presence of a compact remnant (neutron star or black hole).Comment: 28 pages, 8 figures. Accepted to the Astrophysical Journal, to appear in 20 April 2006 issue. Full resolution figures are posted at: http://stevenf.asu.edu/figure

Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways

BackgroundDeveloping new bioprocesses to produce chemicals and fuels with reduced production costs will greatly facilitate the replacement of fossil-based raw materials. In most fermentation bioprocesses, the feedstock usually represents the highest cost, which becomes the target for cost reduction. Additionally, the biorefinery concept advocates revenue growth from the production of several compounds using the same feedstock. Taken together, the production of bio commodities from low-cost gas streams containing CO, CO2, and H2, obtained from the gasification of any carbon-containing waste streams or off-gases from heavy industry (steel mills, processing plants, or refineries), embodies an opportunity for affordable and renewable chemical production. To achieve this, by studying non-model autotrophic acetogens, current limitations concerning low growth rates, toxicity by gas streams, and low productivity may be overcome. The Acetobacterium wieringae strain JM is a novel autotrophic acetogen that is capable of producing acetate and ethanol. It exhibits faster growth rates on various gaseous compounds, including carbon monoxide, compared to other Acetobacterium species, making it potentially useful for industrial applications. The species A. wieringae has not been genetically modified, therefore developing a genetic engineering method is important for expanding its product portfolio from gas fermentation and overall improving the characteristics of this acetogen for industrial demands.ResultsThis work reports the development and optimization of an electrotransformation protocol for A. wieringae strain JM, which can also be used in A. wieringae DSM 1911, and A. woodii DSM 1030. We also show the functionality of the thiamphenicol resistance marker, catP, and the functionality of the origins of replication pBP1, pCB102, pCD6, and pIM13 in all tested Acetobacterium strains, with transformation efficiencies of up to 2.0 × 103 CFU/μgDNA. Key factors affecting electrotransformation efficiency include OD600 of cell harvesting, pH of resuspension buffer, the field strength of the electric pulse, and plasmid amount. Using this method, the acetone production operon from Clostridium acetobutylicum was efficiently introduced in all tested Acetobacterium spp., leading to non-native biochemical acetone production via plasmid-based expression.ConclusionsA. wieringae can be electrotransformed at high efficiency using different plasmids with different replication origins. The electrotransformation procedure and tools reported here unlock the genetic and metabolic manipulation of the biotechnologically relevant A. wieringae strains. For the first time, non-native acetone production is shown in A. wieringae

Terminal velocities of luminous, early-type SMC stars

Ultraviolet spectra from the Space Telescope Imaging Spectrograph (STIS) are used to determine terminal velocities for 11 O and B-type giants and supergiants in the Small Magellanic Cloud (SMC) from the Si IV and C IV resonance lines. Using archival data from observations with the Goddard High-Resolution Spectrograph and the International Ultraviolet Explorer telescope, terminal velocities are obtained for a further five B-type supergiants. We discuss the metallicity dependence of stellar terminal velocities, finding no evidence for a significant scaling between Galactic and SMC metallicities for Teff < 30,000 K, consistent with the predictions of radiation driven wind theory for supergiant stars. A comparison of the $v_\infty / v_{esc}$ ratio between the SMC and Galactic samples, while consistent with the above statement, emphasizes that the uncertainties in the distances to galactic O-stars are a serious obstacle to a detailed comparison with theory. For the SMC sample there is considerable scatter in this ratio at a given effective temperature, perhaps indicative of uncertainties in stellar masses.Comment: 28 pages, 8 figures, accepted by ApJ; minor revisions prior to acceptanc