Differences in Apoptosis and Cell Cycle Distribution between Human Melanoma Cell Lines UACC903 and UACC903(+6), before and after UV Irradiation

Introduction of human chromosome 6 into malignant melanoma cell line UACC903 resulted in generation of the chromosome 6-mediated suppressed cell subline UACC903(+6) that displays attenuated growth rate, anchorage-dependency, and reduced tumorigenicity. We have showed that overexpression of a chromosome 6-encoded tumor suppressor gene led to partial suppression to UACC903 cell growth. We now describe the differences in apoptosis and cell cycle between UACC903 and UACC903(+6) before and after UV irradiation. MTT assay revealed 86.92±8.24% of UACC903 cells viable, significantly (p<0.01) higher than 48.76±5.31% of UACC903(+6), at 24 hr after 254-nm UV irradiation (40 J/M2). Before UV treatment, flow cytometry analysis revealed 6.06±0.20% apoptosis in UACC903, significantly (p=0.01) lower than 6.67±0.15% in UACC903(+6). The G0/G1, S and G2/M phase cells of UACC903 were, respectively, 54.10±0.59%, 22.31±0.50% and 16.85±0.25%, all significantly (p<0.01) different from the corresponding percentages (58.82±0.35%, 20.48±0.05%, and 13.17±0.45%) of UACC903(+6). After the UV treatment, UACC903 cells in apoptosis, G0/G1, S, and G2/M became 12.59±0.17%, 38.90±0.67%, 19.74±0.70%, and 27.01±0.66%, respectively, while UACC903(+6) cells were 24.16±0.48%, 37.97±0.62%, 19.20±0.52%, and 15.69±0.14%. TUNEL assay revealed 2.31±0.62% apoptosis in UACC903, significantly (p<0.01) lower than 9.60±1.14% of UACC903(+6), and a linear and exponential increase of apoptosis, respectively, in response to the UV treatment. These results indicate that UACC903(+6) cells have a greater tendency to undergo apoptosis and are thus much more sensitive to UV irradiation. Our findings further suggest a novel mechanism for chromosome 6-mediated suppression of tumorigenesis and metastasis, i.e., through increased cell death

Damping Methods for Resonances Caused by LCL-Filter-Based Current-Controlled Grid-Tied Power Inverters: An Overview

Grid-tied voltage source inverters using LCL filter have been widely adopted in distributed power generation systems (DPGSs). As high-order LCL filters contain multiple resonant frequencies, switching harmonics generated by the inverter and current harmonics generated by the active/passive loads would cause the system resonance, and thus the output current distortion and oscillation. Such phenomenon is particularly critical when the power grid is weak with the unknown grid impedance. In order to stabilize the operation of the DPGS and improve the waveform of the injected currents, many innovative damping methods have been proposed. A comprehensive overview on those contributions and their classification on the inverter- and grid-side damping measures are presented. Based on the concept of the impedance-based stability analysis, all damping methods can ensure the system stability by modifying the effective output impedance of the inverter or the effective grid impedance. Classical damping methods for industrial applications will be analyzed and compared. Finally, the future trends of the impedance-based stability analysis, as well as some promising damping methods, will be discussed

An Accurate Switching Transient Analytical Model for GaN HEMT under the Influence of Nonlinear Parameters

The Gallium Nitride high electron mobility transistor (GaN HEMT) has been considered as a potential power semiconductor device for high switching speed and high power density application since its commercialization. Compared with the traditional Si transistors, GaN HEMT has faster switching speed and lower on-off loss. As a result, it is more sensitive to the nonlinear parameters due to the fast switching speed. The subsequent voltage and current overshooting will affect the efficiency and safety of the GaN HEMT and power electronic systems. In this paper, an accurate switching transient analytical model for GaN HEMT is proposed, which considers the effects of parasitic inductances, nonlinear junction capacitances and nonlinear transconductance. The model characteristic of turn-ON process and turn-OFF process is illustrated in detail, and the equivalent circuits are derived for each switching transition. The accuracy of the proposed model can be verified by comparing the predicted switching waveform and switching loss with that of the experimental results based on the double pulse test (DPT) circuit. Compared with the conventional model, the proposed model is more accurate and matches better with the experimental results than the conventional model. Finally, this model can be used for analyzing the influences of gate resistance, nonlinear junction capacitances, and parasitic inductances on switching transient waveform and refining calculation switching loss

Multi-Resonant-Based Sliding Mode Control of DFIG-Based Wind System under Unbalanced and Harmonic Network Conditions

In general, the integral sliding mode control (ISMC) with an integral sliding surface would lead to tracking errors under unbalanced and harmonic grid voltage conditions. In order to eliminate tracking errors under these conditions, multi-resonant items are added to the conventional integral sliding surface in the proposed strategy, which can be called multi-resonant-based sliding mode control (MRSMC). A comparison of tracking precision on the ISMC and MRSMC is analyzed. In order to regulate the system powers directly, the errors of instantaneous active and reactive powers are selected as the state variables. Finally, the output current harmonics and a majority of the doubly-fed induction generator&rsquo;s (DFIG) electromagnetic torque pulsations can be removed under unbalanced and harmonic grid voltage conditions. Simulation and experimental results are presented to verify the correctness and effectiveness of the proposed strategy