Effect of miR-200c on nasopharyngeal carcinoma and the probable molecular regulatory mechanism involved

Purpose: To determine the effect of micro-ribonucleic acid-200c (miR-200c) on biological function of nasopharyngeal carcinoma, and the likely molecular regulatory mechanism involved.Methods: Thirty (30) nasopharyngeal carcinoma tissues and para-cancerous normal tissues were taken from patients undergoing surgery in Maternal and Child Health Hospital of Hubei Province, Wuhan from September 2018 to January 2020. The expression levels of miR-200c in the two types of tissues were determined. Cells of human nasopharyngeal carcinoma cell line HNE1 were cultured to about 70 % growth prior to transfection with blank plasmid, PINI and miR-200c analogs. After 48 h of culture, control group, PINI group, and miR-200c over-expression + PINI group were obtained. The expression levels of PINI and changes in centrosomes in each group were measured, and changes in cell migration in each group were determined using Transwell migration assay.Results: Compared with para-cancerous normal tissues, the expression level of miR-200c in nasopharyngeal carcinoma was  significantly increased (p < 0.01). Compared with the control group, the PINI expression level and cell migration ability in miR-200c overexpression tissue were markedly decreased, while the percentage of extra centrosomes was markedly increased. Compared to miR-200c over-expression tissue, the PINI expression level and cell migration ability in the miR-200c overexpression + PINI group were markedly raised, while the percentage of extra-central somatic cells was significantly decreased (p < 0.05).Conclusion: MiR-200c may inhibit the migration of nasopharyngeal carcinoma cells by inhibiting the expression of PINI and inhibiting abnormal expansion of centrosomes. Keywords: MiR-200c, Nasopharyngeal carcinoma, Biological function, Molecular regulatory mechanis

Localization of Fusobacterium nucleatum in oral squamous cell carcinoma and its possible directly interacting protein molecules: A case series

Introduction. While 15 to 20% of cancers are associated with microbial infection, the relationship between oral microorganisms and oral squamous cell carcinoma (OSCC) remains unclear. The location of bacteria in a tumor is closely related to its carcinogenic mechanism. The aim of this study was to analyse bacterial diversity in clinical OSCC tissue samples and tumor distant normal tissues, locate target bacteria, and search for proteins that may interact with target bacteria. Materials and Methods. The 16S rDNA method was used to analyse bacterial diversity in clinical OSCC tissue samples and tumor distant normal tissues. Correlations between Fusobacterium abundance and clinicopathological characteristics were analysed using the χ2 test. The position of target bacteria was analysed by fluorescence in situ hybridization (FISH), and the expression of CK, CD31, CD45, CD68, cyclin D1, βcatenin, E-cadherin, NF-κB, and HIF-1α was analysed by immunohistochemistry (IHC) in OSCC tumor tissues and tumor distant normal tissues. Results. The 16S rDNA results showed that the detected amount of Fusobacterium in OSCC tumor tissues was significantly larger than that in tumor distant normal tissues. High expression of Fusobacterium was significantly correlated with the lifestyle-related oral risk habits, including smoking (p=0.036) and alcohol consumption (p=0.022), but did not correlate with patient sex, age, tumor laterality, tumor size, grade or TNM stage. Fusobacterium nucleatum was enriched in tumor stroma, where CD31+ blood vessels and inflammatory cells (including CD45+ leukocytes and CD68+ macrophages) were densely distributed. Cyclin D1 was mainly expressed in the nucleus of tumor cells. β-catenin was expressed in the tumor cell membrane and was positively expressed in tumor interstitial vascular endothelial cells. E-cadherin was mainly expressed in tumor cell membranes. NF-κB was positively expressed in the cytoplasm of tumor cells, tumor interstitial cells and myo-fibrocytes. HIF-1α was mainly expressed in the cytoplasm of tumor interstitial cells. HIF-1α was highly expressed where Fusobacterium nucleatum was densely distributed. Conclusion. According to our study, the detected amount of Fusobacterium in OSCC tumor tissues was significantly larger than that in tumor distant normal tissues, and Fusobacterium nucleatum might aggravate inflammation and hypoxia by interacting with NF-κB and HIF-1α in OSCC

Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts

<p>Abstract</p> <p>Background</p> <p>The purpose of this study was to assess the expression levels for TβRI, TβRII, and TβRIII in epithelial layers of oral premalignant lesions (oral leukoplakia, OLK) and oral squamous cell carcinoma (OSCC), as well as in oral carcinoma-associated fibroblasts (CAFs), with the final goal of exploring the roles of various types of TβRs in carcinogenesis of oral mucosa.</p> <p>Methods</p> <p>Normal oral tissues, OLK, and OSCC were obtained from 138 previously untreated patients. Seven primary human oral CAF lines and six primary normal fibroblast (NF) lines were established successfully via cell culture. The three receptors were detected using immunohistochemical (IHC), quantitative RT-PCR, and Western blot approaches.</p> <p>Results</p> <p>IHC signals for TβRII and TβRIII in the epithelial layer decreased in tissue samples with increasing disease aggressiveness (P < 0.05); no expression differences were observed for TβRI, in OLK and OSCC (P > 0.05); and TβRII and TβRIII were significantly downregulated in CAFs compared with NFs, at the mRNA and protein levels (P < 0.05). Exogenous expression of TGF-β1 led to a remarkable decrease in the expression of TβRII and TβRIII in CAFs (P < 0.05).</p> <p>Conclusion</p> <p>This study provides the first evidence that the loss of TβRII and TβRIII expression in oral epithelium and stroma is a common event in OSCC. The restoration of the expression of TβRII and TβRIII in oral cancerous tissues may represent a novel strategy for the treatment of oral carcinoma.</p

WASp-deficient B cells play a critical, cell-intrinsic role in triggering autoimmunity

In a manner dependent on CD4 T cell help and Toll-like receptor signals, B cells lacking WASp induce autoantibody production and autoimmune disease in mice

Review of accumulative failure of winding insulation subjected to repetitive impulse voltages

The premature failure phenomenon that occurs in solid insulation under the application of repetitive impulse voltages is known as the accumulative effect of impulse voltages on a solid dielectric. The accumulative failure of winding insulation is considered to be a core problem resulting from this phenomenon. The main problems include the accumulative failure of motor winding insulation under pulse-width modulation impulses and the accumulative failure of transformer winding insulation under the application of overvoltages, and these problems represent new challenges in the safe and stable operation of power grids. With regards to the above problems, this study reviews the results of recent research on the accumulative failure characteristics and mechanisms of winding insulation when subjected to repetitive impulse voltages. The challenges faced are discussed at the end of this study

Overturning the “doing-comes-before-talking” mentality, building China’s leadership in global science governance

A 'Neican' policy recommendation to China's Ministry of Science and Technology on promoting accountable science and research transparency

Effect of Fe3O4 nanoparticles on space charge distribution in propylene carbonate under impulse voltage

Addition of nanoparticles of the ferromagnetic material Fe3O4 can increase the positive impulse breakdown voltage of propylene carbonate by 11.65%. To further investigate the effect of ferromagnetic nanoparticles on the space charge distribution in the discharge process, the present work set up a Kerr electro-optic field mapping measurement system using an array photodetector to carry out time-continuous measurement of the electric field and space charge distribution in propylene carbonate before and after modification. Test results show that fast electrons can be captured by Fe3O4 nanoparticles and converted into relatively slow, negatively charged particles, inhibiting the generation and transportation of the space charge, especially the negative space charge

A Robust Random Forest-Based Approach for Heart Rate Monitoring Using Photoplethysmography Signal Contaminated by Intense Motion Artifacts

The estimation of heart rate (HR) based on wearable devices is of interest in fitness. Photoplethysmography (PPG) is a promising approach to estimate HR due to low cost; however, it is easily corrupted by motion artifacts (MA). In this work, a robust approach based on random forest is proposed for accurately estimating HR from the photoplethysmography signal contaminated by intense motion artifacts, consisting of two stages. Stage 1 proposes a hybrid method to effectively remove MA with a low computation complexity, where two MA removal algorithms are combined by an accurate binary decision algorithm whose aim is to decide whether or not to adopt the second MA removal algorithm. Stage 2 proposes a random forest-based spectral peak-tracking algorithm, whose aim is to locate the spectral peak corresponding to HR, formulating the problem of spectral peak tracking into a pattern classification problem. Experiments on the PPG datasets including 22 subjects used in the 2015 IEEE Signal Processing Cup showed that the proposed approach achieved the average absolute error of 1.65 beats per minute (BPM) on the 22 PPG datasets. Compared to state-of-the-art approaches, the proposed approach has better accuracy and robustness to intense motion artifacts, indicating its potential use in wearable sensors for health monitoring and fitness tracking