Gene Prediction System ·

With the increasingly popularity of genome sequencing, transfonning such raw sequence data into knowledge remains a hard task. This project will develop an application for gene prediction using development tools such as Perl and PHP. This project also will identify stretches of sequence for genomic DNA that is biologically functional including protein coding regions. There are 3 steps involve which are transcription, splicing and translation. Transcription is the process of copying DNA to RNA Meanwhile, splicing is modification of genetic ) infonnation after a few transcriptions. It will determine introns (useless infonnation) and exons (useful infonnation) in DNA sequence. Hidden Markov Model which is a mathematical functional will be used in this step in order to predict the DNA sequence. The introns will be removed and exons will be combined together to produce mRNA. The final step is translation which is the process by which mRNA is translated into proteins. The protein sequence is the final output of Gene Prediction System. More knowledge on gene prediction will be explored in order to get better unde~tanding of how gene prediction works

Gene Prediction System ·

With the increasingly popularity of genome sequencing, transfonning such raw sequence data into knowledge remains a hard task. This project will develop an application for gene prediction using development tools such as Perl and PHP. This project also will identify stretches of sequence for genomic DNA that is biologically functional including protein coding regions. There are 3 steps involve which are transcription, splicing and translation. Transcription is the process of copying DNA to RNA Meanwhile, splicing is modification of genetic ) infonnation after a few transcriptions. It will determine introns (useless infonnation) and exons (useful infonnation) in DNA sequence. Hidden Markov Model which is a mathematical functional will be used in this step in order to predict the DNA sequence. The introns will be removed and exons will be combined together to produce mRNA. The final step is translation which is the process by which mRNA is translated into proteins. The protein sequence is the final output of Gene Prediction System. More knowledge on gene prediction will be explored in order to get better unde~tanding of how gene prediction works

Analisis dan penyaringan data lewah interaksi kelompok bes berikatan hidrogen dalam struktur RNA 3-dimensi

Susun atur 3-dimensi (3D) yang sama boleh disalah cerap sebagai berbeza dari sudut penglihatan yang berlainan. Bagi makromolekul biologi, permasalahan ini juga dihadapi oleh algoritma pencarian susun atur 3D. Keputusan hasil larian yang sama akan diperoleh berulang kali (data lewah) kerana hasil tersebut boleh mempunyai susun atur jujukan berbeza. Permasalahan ini tidak ditemui di dalam pencarian jujukan. Dalam kajian ini, dua kaedah untuk menyaring data lewah tersebut telah dibangunkan dan dibandingkan iaitu kod Prüfer (berasaskan teori graf) dan kaedah saringan data lewah (dibangunkan secara khusus untuk kajian). Model hasil carian pula adalah menggunakan COnnection tables Graphs for Nucleic ACids (COGNAC) bagi pencarian interaksi kelompok bes berikatan hidrogen. Perbandingan yang dilakukan menunjukkan bahawa kaedah saringan data lewah mampu untuk mengenal pasti dan menyaring antara 50.5% sehingga 80% data lewah daripada hasil larian asal COGNAC berbanding kod Prüfer yang hanya mengenal pasti dan menyaring 50% data lewah daripada hasil larian asal COGNAC. Oleh itu, kaedah saringan data lewah telah diimplementasi ke dalam COGNAC. Selain itu, kaedah saringan data lewah ini juga boleh diguna pakai untuk algoritma yang tidak bergantung kepada jujukan bagi pencarian motif 3D dalam struktur protein