A study of the antimicrobial activity of combined black pepper and cinnamon essential oils against Escherichia fergusonii in traditional African yoghurt

© 2021 The Authors. Published by MDPI. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.3390/foods10112847The antimicrobial activity of the essential oils of black pepper (BPE) and cinnamon bark (CE) extracts against E. fergusonii was assessed in pasteurized full cream milk during and post-fermentation. The milk was fermented with 1% (v/v) of Lactobacillus delbrueckii subspecies bulgaricus (NCIMB 11778) and Streptococcus thermophilus (NCIMB 10387) (approx. 106 cfu/mL each) and incubated and stored at 25 °C for 5 days (144 h) or at 43 °C for 24 h and then stored at 25 °C for 120 h. The milk was spiked with E. fergusonii at the start of fermentation by the lactic acid bacteria (pre-fermentation contamination) for after fermentation (post fermentation contamination). BPE and CE were applied at concentrations based on their minimum inhibitory concentration of 0.5% and 0.25% respectively as follows: 0.5% BPE alone; 0.125% BPE with 0.1875% CE; 0.25% BPE with 0.125% CE; 0.375% BPE with 0.0625% CE; 0.25% CE alone. Results showed that during fermentation at 25 °C, E. fergusonii grew to a similar level (approx. 109 CFU/mL) in control samples and 108 CFU/mL when BPE or CE were added alone. Whereas, in the samples with the combined essential oils, the bacterium grew to 106–107 CFU/mL only. During the milk fermentation at 43 °C, E. fergusonii grew to approx. 109 CFU/mL in samples without treatment. However, it was not detected in samples containing mixed BPE with CE after 8, 10 and 12 h of fermentation. Subsequent storage at 25 °C resulted in undetectable levels of the bacterium in all the samples treated with BPE or CE after 24 h of storage. These results indicated that BPE in combination with CE reduced growth during fermentation and was bactericidal during storage

The effect of combined clove and cinnamon extracts on growth and survival of escherichia fergusonii and salmonella typhimurium in milk pre and post fermentation

© 2022 The Authors. Published by Fortune Journals. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://www.fortunejournals.com/journal-of-food-science-and-nutrition-research-home-fjfsnr.phpThe antimicrobial activities of extracts of clove buds (CL) and cinnamon bark (CE) were investigated individually and in combination in fermenting and fermented full cream milk against Escherichia fergusonii and Salmonella typhimurium. Clove and cinnamon were extracted for their essential oils (EOs) and eugenol and cinnamaldehyde were the major components representing 60-80% of the total oils Preheated milk was inoculated with 1% (v/v) of a mixed culture of Lactobacillus delbrueckii subspecies bulgaricus (NCIMB 11778) and Streptococcus thermophilus (NCIMB 10387) and incubated at 25 or 37oC for 24 h. E. fergusonii or S. typhimurium (3 x 105 CFU/mL) were introduced into the milk pre - or post-fermentation. CL and CE were added at the same time as the pathogens based on their Minimum Inhibitory Concentrations of 0.25% for both pathogens as follows: CL and CE at 0.25% each alone; 0.0625% CL/0.1875% CE; 0.125% CL/ 0.125% CE; and 0.1875% CL/0.0625% CE. When added at the start of fermentation at 25oC, the CL and CE combinations inhibited the growth of E. fergusonii, and S. typhimurium, whereas at 37oC and associated with more rapid and higher acidification, the CL and CE combinations showed a marked antimicrobial activity against both pathogens. In post fermentation inoculated milk, survival of E. fergusonii and S. typhimurium was reduced by all CL and/ or CE treatments and were undetectable in samples containing 0.0625% CE with 0.1875% CL and 0.1875% CE with 0.0625% CL within 48 to 72 h of storage. The extent of the effect was most marked in the samples fermented at 37oC compared to 25oC and related to the pH after fermentation of 4.2 ±0.2 and 5.0 ±0.2 respectively. The use of these extracts both during fermentation and storage have the potential to enhance the microbiological safety of these products

Zebrafish promoter microarrays identify actively transcribed embryonic genes.

We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Changing environment at the Late Upper Palaeolithic ite of Lynx Cave, North Wales

Lynx Cave is one of a handful of locations in North Wales that provide evidence of Late Upper Palaeolithic huntergatherers at the end of the last ice-age. With the region being recolonized at a time of rapid environmental change there is a need to develop on-site palaeoenvironmental records that are directly linked to the archaeology in order to further understanding of the environments and landscapes that these hunter-gatherer groups experienced. Through carbon (δ13C), nitrogen (δ15N) and sulphur (δ34S) stable isotope analysis of animal bones we explore the environmental conditions during the human occupation of Lynx Cave. Analysis of the data indicates the faunal isotope results cluster into three distinct groupings, which when considered in light of the species composition, radiocarbon dates, sample layer provenance and known temporal patterns in herbivore isotope data from Northern Europe, are likely to relate to GI-1cba (the Allerød period) around 13,700-13,000 cal BP, GI-1cba/GS-1 (the Late Allerød/ early Younger Dryas period) around 13,100-12,800 cal BP, and the Bronze Age. The isotope data indicates that the Late Upper Palaeolithic or Late Palaeolithic occupations occurred in an open landscape in which soils were undergoing changing hydrological conditions linked to ice sheet melt and permafrost thaw process and subsequent recovery. The evidence of butchery marks on the faunal remains from both Late Glacial isotope clusters, along with the disparate radiocarbon dates and the presence of three hearths, support the idea of very short-term episodic use of the cave over an extended time period

Behavioral and Other Phenotypes in a Cytoplasmic Dynein Light Intermediate Chain 1 Mutant Mouse

The cytoplasmic dynein complex is fundamentally important to all eukaryotic cells for transporting a variety of essential cargoes along microtubules within the cell. This complex also plays more specialized roles in neurons. The complex consists of 11 types of protein that interact with each other and with external adaptors, regulators and cargoes. Despite the importance of the cytoplasmic dynein complex, we know comparatively little of the roles of each component protein, and in mammals few mutants exist that allow us to explore the effects of defects in dynein-controlled processes in the context of the whole organism. Here we have taken a genotype-driven approach in mouse (Mus musculus) to analyze the role of one subunit, the dynein light intermediate chain 1 (Dync1li1). We find that, surprisingly, an N235Y point mutation in this protein results in altered neuronal development, as shown from in vivo studies in the developing cortex, and analyses of electrophysiological function. Moreover, mutant mice display increased anxiety, thus linking dynein functions to a behavioral phenotype in mammals for the first time. These results demonstrate the important role that dynein-controlled processes play in the correct development and function of the mammalian nervous system

Human Cortical Neural Stem Cells Expressing Insulin‐Like Growth Factor‐I: A Novel Cellular Therapy for Alzheimer’s Disease

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135291/1/sct3201653379.pd

Influence of Maternal Lifestyle and Diet on Perinatal DNA Methylation Signatures Associated With Childhood Arterial Stiffness at 8 to 9 Years

Increases in aortic pulse wave velocity, a measure of arterial stiffness, can lead to elevated systolic blood pressure and increased cardiac afterload in adulthood. These changes are detectable in childhood and potentially originate in utero, where an adverse early life environment can alter DNA methylation patterns detectable at birth. Here, analysis of epigenome-wide methylation patterns using umbilical cord blood DNA from 470 participants in the Southampton’s Women’s Survey identified differential methylation patterns associated with systolic blood pressure, pulse pressure, arterial distensibility, and descending aorta pulse wave velocity measured by magnetic resonance imaging at 8 to 9 years. Perinatal methylation levels at 16 CpG loci were associated with descending aorta pulse wave velocity, with identified CpG sites enriched in pathways involved in DNA repair (P=9.03×10−11). The most significant association was with cg20793626 methylation (within protein phosphatase, Mg2+/Mn2+ dependent 1D; β=−0.05 m/s/1% methylation change, [95% CI, −0.09 to −0.02]). Genetic variation was also examined but had a minor influence on these observations. Eight pulse wave velocity-linked dmCpGs were associated with prenatal modifiable risk factors, with cg08509237 methylation (within palmitoyl-protein thioesterase-2) associated with maternal oily fish consumption in early and late pregnancy. Lower oily fish consumption in early pregnancy modified the relationship between methylation and pulse wave velocity, with lower consumption strengthening the association between cg08509237 methylation and increased pulse wave velocity. In conclusion, measurement of perinatal DNA methylation signatures has utility in identifying infants who might benefit from preventive interventions to reduce risk of later cardiovascular disease, and modifiable maternal factors can reduce this risk in the child

An integrated clinical program and crowdsourcing strategy for genomic sequencing and Mendelian disease gene discovery.

Despite major progress in defining the genetic basis of Mendelian disorders, the molecular etiology of many cases remains unknown. Patients with these undiagnosed disorders often have complex presentations and require treatment by multiple health care specialists. Here, we describe an integrated clinical diagnostic and research program using whole-exome and whole-genome sequencing (WES/WGS) for Mendelian disease gene discovery. This program employs specific case ascertainment parameters, a WES/WGS computational analysis pipeline that is optimized for Mendelian disease gene discovery with variant callers tuned to specific inheritance modes, an interdisciplinary crowdsourcing strategy for genomic sequence analysis, matchmaking for additional cases, and integration of the findings regarding gene causality with the clinical management plan. The interdisciplinary gene discovery team includes clinical, computational, and experimental biomedical specialists who interact to identify the genetic etiology of the disease, and when so warranted, to devise improved or novel treatments for affected patients. This program effectively integrates the clinical and research missions of an academic medical center and affords both diagnostic and therapeutic options for patients suffering from genetic disease. It may therefore be germane to other academic medical institutions engaged in implementing genomic medicine programs