Adsorption characteristics of bovine serum albumin onto alumina with a specific crystalline structure

Bone cement containing alumina particles with a specific crystalline structure exhibits the ability to bond with bone. These particles (AL-P) are mainly composed of delta-type alumina (δ-Al2O3). It is likely that some of the proteins present in the body environment are adsorbed onto the cement and influence the expression of its bioactivity. However, the effect that this adsorption of proteins has on the bone-bonding mechanism of bone cement has not yet been elucidated. In this study, we investigated the characteristics of the adsorption of bovine serum albumin (BSA) onto AL-P and compared them with those of its adsorption onto hydroxyapatite (HA), which also exhibits bone-bonding ability, as well as with those of adsorption onto alpha-type alumina (α-Al2O3), which does not bond with bone. The adsorption characteristics of BSA onto AL-P were very different from those onto α-Al2O3 but quite similar to those onto HA. It is speculated that BSA is adsorbed onto AL-P and HA by interionic interactions, while it is adsorbed onto α-Al2O3 by electrostatic attraction. The results suggest that the specific adsorption of albumin onto implant materials might play a role in the expression of the bone-bonding abilities of the materials

Supplementary material for the article: Fukushi, K.; Fujita, Y.; Nonogaki, J.; Tsujimoto, J.-I.; Hattori, T.; Inui, H.; Beškoski, V. P.; Hotta, H.; Hayashi, M.; Nakano, T. Capillary Zone Electrophoresis Determination of Fluoride in Seawater Using Transient Isotachophoresis. Analytical and Bioanalytical Chemistry 2018, 410 (6), 1825–1831. https://doi.org/10.1007/s00216-017-0838-0

Supplementary material for: [https://doi.org/10.1007/s00216-017-0838-0]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2083

Self-trapped electrons and holes in PbBr2_2 crystals

We have directly observed self-trapped electrons and holes in PbBr$_{2}$ crystals with electron-spin-resonance (ESR) technique. The self-trapped states are induced below 8 K by two-photon interband excitation with pulsed 120-fs-width laser light at 3.10 eV. Spin-Hamiltonian analyses of the ESR signals have revealed that the self-trapping electron centers are the dimer molecules of Pb$_2$$^{3+}$ along the crystallographic a axis and the self-trapping hole centers are those of Br$_2$$^-$ with two possible configurations in the unit cell of the crystal. Thermal stability of the self-trapped electrons and holes suggests that both of them are related to the blue-green luminescence band at 2.55 eV coming from recombination of spatially separated electron-hole pairs.Comment: 8 pages (7 figures, 2 tables), ReVTEX; revised the text and figures 1, 4, and

Supplementary material for the article: Fukushi, K.; Fujita, Y.; Nonogaki, J.; Tsujimoto, J.-I.; Hattori, T.; Inui, H.; Beškoski, V. P.; Hotta, H.; Hayashi, M.; Nakano, T. Capillary Zone Electrophoresis Determination of Fluoride in Seawater Using Transient Isotachophoresis. Analytical and Bioanalytical Chemistry 2018, 410 (6), 1825–1831. https://doi.org/10.1007/s00216-017-0838-0

Supplementary material for: [https://doi.org/10.1007/s00216-017-0838-0]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2083

West Nile Virus Inhibits the Signal Transduction Pathway of Alpha Interferon

West Nile virus (WNV) is a human pathogen that can cause neurological disorders, including meningoencephalitis. Experiments with mice and mammalian cell cultures revealed that WNV exhibited resistance to the innate immune program induced by alpha interferon (IFN-α). We have investigated the nature of this inhibition and have found that WNV replication inhibited the activation of many known IFN-inducible genes, because it prevented the phosphorylation and activation of the Janus kinases JAK1 and Tyk2. As a consequence, activation of the transcription factors STAT1 and STAT2 did not occur in WNV-infected cells. Moreover, we demonstrated that the viral nonstructural proteins are responsible for this effect. Thus, our results provided an explanation for the observed resistance of WNV to IFN-α in cells of vertebrate origin