Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts

Comparison of Two Diffusers in a Transonic Centrifugal Compressor

Flow mechanisms suppressing the flow separation in two diffusers, a low-solidity cascade diffuser and a vaned diffuser with additional small vanes near the inlet, were compared mainly by numerical simulation. As the superiority of the low-solidity cascade diffuser was expected, a series of experiments was conducted using a transonic centrifugal compressor with a maximum pressure ratio of 7. The performance of the compressor with the vaned diffuser was comparable to that of the low-solidity cascade diffuser only between the surge point and the design flowrate at a pressure ratio of 3.5. The maximum flowrate of the vaned diffuser was lower than that of the low-solidity cascade diffuser. At higher rotational speeds, the pressure ratio at the surge point, the efficiency, and the flow range of the low-solidity cascade diffuser exceded those of a vaned diffuser at a pressure ratio of 3.5