A propos de l'exégèse des manuscrits en Tchécoslovaquie

Lubomira Havlikova, Kabinet pro studia řecká, řimská a latinská, ČSAV, Prague, CzechoslovakiaThis paper was presented at the "International Data Bases for Medieval Manuscript Studies" conference at Katholieke Universiteit, Nijmegen, September 1987.A brief list is given of the repositories in Czechoslovakia that contain manuscripts and any inventories or catalogs of these manuscripts are noted. A selected bibliography of print catalogs is included

Fatigue, mood disorders and sleep problems in patients with Parkinson's disease

De Ziekte van Parkinson (PD) is een langzaam progressieve ziekte gekarakteriseerd door zijn motorische en meer recentelijk herkende nietmotorische verschijnselen. Dit proefschrift richt zich op vermoeidheid, slaap problemen en stemmingsstoornissen, op de relaties tussen deze niet- motorische verschijnselen en hun invloed op de levenskwaliteit van Parkinsonpatiënten. Levenskwaliteit is een van de meest belangrijke maten om op een geïntegreerde manier de gevolgen van een chronische zieke te evalueren. Zie verder: Samenvatting

Liquid extraction surface analysis mass spectrometry of microbes: towards point-of-care diagnosis for wound infection

Trauma is one of the leading causes of death of people under age 49, while complications associated with the wound infection are the primary cause of death in the first day of injury. Microbial infections are mostly caused by bacteria and fungi commonly present in the hospital environment and these are associated with antibiotic and antifungal resistance. Rapid and accurate microbial identification is therefore of high importance. Current diagnostics takes hours or even days to obtain the result. This work uses liquid extraction surface analysis mass spectrometry (LESA MS) for top-down (TD) analysis of proteins in clinically important microbes including the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.) and yeast Candida glabrata. First, the ESKAPE microbes and their two close clinical isolates were grown on simple substrates and subjected to LESA MS. Searches against individual protein databases resulted in identification of 24 proteins from 37 MS/MS mass spectra, while searching against multiple databases determined identification success rate at protein and species level to be 79%. A biofilm model of P. aeruginosa and C. glabrata was cultured, resulting in identification of three P. aeruginosa proteins. Next, a LESA MS workflow was developed for analysis of wounded in vitro three-dimensional living skin models inoculated with four of the ESKAPE species and C. glabrata, resulting in assignment of seven human, nine bacterial and one yeast proteins. The LESA MS workflow was subsequently applied to ex vivo human skin grafts including those inoculated with S. aureus, and two human skin proteins were identified from both types of samples. Lastly, LESA MS was coupled to state-of-art cylindrical high-field asymmetric waveform ion mobility spectrometry (FAIMS) and mass spectrometer and a high-throughput approach for TD identification of proteins in bacteria was developed. In total, the high-throughput TD LESA FAIMS analysis of Escherichia coli K12 and four ESKAPE species allowed identification of 179 proteins and 277 proteoforms. The results presented in this thesis suggest that LESA MS and LESA FAIMS MS are tools capable of relatively fast extraction of proteins from microbes cultured on a variety of substrates with their subsequent tandem MS identification, showing potential of LESA as a future in situ diagnostic

Přechod do dospělosti dlouhodobě nezaměstnaných absolventů učebních oborů z Brna a okolí

The aim of this article is to provide insight into the circumstances of long-term unemployed graduates of (mostly) non-GCSE vocational programmes from the perspective of their transition to adulthood. The analytical framework used for this research is life course theory, according to which it is possible to approach the transition from youth to adulthood as a multiple transition. This point corresponds well with reality because young people follow at least three trajectories on their way to adulthood: from school to work, from family of origin to family of procreation, and from dependence to independence. The data necessary for the analysis were collected through repeated biographical and semi-structured interviews with 14 long-term unemployed graduates of non-GCSE vocational programmes and 6 employed graduates of vocational programmes as a reference group. Their implicit theories of adulthood, progress on the path to adulthood, and everyday strategies were examined in a qualitative data analysis, with special attention paid to contextual aspects. As for the dominant form of transition, the author found that long-term unemployment has a delaying impact on the transition to adulthood, above all owing to financial strain. These people suffer from prolonged economical dependency on their parents and remain at the threshold of the socially constructed path to adulthood. Typically there social status is vague

Identification of neuroactive steroids and their precursors and metabolites in adult male rat brain

Steroids in the brain arise both from local synthesis and from peripheral sources and have a variety of effects on neuronal function. However, there is little direct chemical evidence for the range of steroids present in brain or of the pathways for their synthesis and inactivation. This information is a prerequisite for understanding the regulation and function of brain steroids. After extraction from adult male rat brain, we have fractionated free steroids and their sulfate esters and then converted them to heptafluorobutyrate or methyloxime-trimethylsilyl ether derivatives for unequivocal identification and assay by gas chromatography analysis and selected ion monitoring mass spectrometry. In the free steroid fraction, corticosterone, 3 alpha, 5 alpha-tetrahydrodeoxycorticosterone, testosterone, and dehydroepiandrosterone were found in the absence of detectable precursors usually found in endocrine glands, indicating peripheral sources and/or alternative synthetic pathways in brain. Conversely, the potent neuroactive steroid 3 alpha, 5 alpha-tetrahydroprogesterone ( allopregnanolone) was found in the presence of its precursors pregnenolone, progesterone, and 5 alpha-dihydroprogesterone. Furthermore, the presence of 3 alpha-, 11 alpha-, 17 alpha-, and 20 alpha-hydroxylated metabolites of 3 alpha, 5 alpha-tetrahydroprogesterone implicated possible inactivation pathways for this steroid. The 20 alpha-reduced metabolites could also be found for pregnenolone, progesterone, and 5 alpha-dihydroprogesterone, introducing a possible regulatory diversion from the production of 3 alpha, 5 alpha-tetrahydroprogesterone. In the steroid sulfate fraction, dehydroepiandrostrone sulfate was identified but not pregnenolone sulfate. Although pharmacologically active, identification of the latter appears to be an earlier methodological artifact, and the compound is thus of doubtful physiological significance in the adult brain. Our results provide a basis for elucidating the origins and regulation of brain steroids