27. Jahrestagung der WEFTA (Western European Fish Technologists’ Association)

Die 27. Jahrestagung der WEFTA fand vom 20.-22.10.1997 in Madrid, Spanien, statt. Organisiert wurde diese WEFTA-Tagung von J. Boderias und den Mitarbeitern des Instituto des Frio in Madrid. Das Interesse an dieser WEFTA-Tagung war besonders groß, dies zeigte sich insbesondere an der Anzahl der angemeldeten Vorträge und Poster sowie an den ca. 130 gemeldeten Teilnehmern aus 16 WEFTA - Mitgliedstaaten und Gästen aus den USA, Argentinien, Israel, Lettland, Mexiko und Venezuela

Relation between TMAOase activity and content of formaldehyde in fillet minces and bellyflap minces from gadoid fishes

Minced fish is a significant component of a number of frozen fishery products like fish fingers, cakes and patties. Predominately minced fish is produced from gadoid species (Alaska pollack, cod, saithe, hake and others) possessing the enzyme trimethylamine oxide demethylase (TMAOase, E.C. 4.1.2.32) (Rehbein and Schreiber 1984). TMAOase catalyses the degradation of trimethylamine oxide (TMAO) to formaldehyde (FA) and dimethylamine (DMA), preferentially during frozen storage of products (Hultin 1992). In most gadoid species light muscle contains only low activity of TMAOase, the activity of red muscle and bellyflaps being somewhat higher. In contrast, the TMAOase activity in blood, kidney and other tissues, residues of which may contaminate minced fish flesh, may be higher for several orders of magnitude (Rehbein and Schreiber 1984)

Effect of diamond microdermabrasion on oily skin: a case report

Background: The history of microdermabrasion dates back to ancient Egypt. The first treatment in Europe occurred in 1985 and has since become a popular method of exfoliation of the superficial layers of skin. Microdermabrasion is an effective method of taking care of the skin of the face and body, bringing about immediate effects in the treatment of deep scars and stretch marks. Aim of the study: To determine the effectiveness of diamond microdermabrasion on a person with hyperuricemia of the sebaceous glands. Material and methods: Based on interviews, a case analysis, and the impact of diamond microdermabrasion on the structure of the skin’s surface, the level of hydration, oiliness and epidermal exfoliation were analyzed. Case report: The subject was a 22-year-old woman struggling with oily skin. A few acne lesions and a large number of blackheads were observed in her nasal area. An interview was conducted prior to the procedure to eliminate any contraindications. The study was conducted within a period of 4 months at 3-week intervals. The Nati Skin Analyzer was used to determine the skin parameters of the patient. Results: After applying a series of treatments using the microdermabrasion apparatus, satisfactory results were obtained in the form of reduced peeled sebum and improved skin hydration in the T and U zones. Conclusions: The study shows that diamond microdermabrasion treatment has a beneficial effect on the patient’s skin condition. Phot ographs before and after the surgery document the positive effects this series of treatments had. There was a decrease in the level of sebum secretion and the elimination of skin changes in the form of acne or open comedones

A sensitive trimethylamine-N-oxide aldolase assay in two steps without deproteinisation

4 páginas, 4 figurasA two-step assay for trimethylamine-N-oxide aldolase (the target enzyme) is described in which the second step, the indicator reaction, is of the enzymatic end-point type. This indicator reaction is carried out at a slightly alkaline pH, outside the target enzyme's active pH range. An effective inactivation of the target enzyme is thus obtained solely by shifting the pH to that of the indicator reaction, thereby avoiding a deproteinisation step. By inserting a downward pH step as the stopping method, many samples may be collected and applied to the indicator reaction simultaneously. These features make the method very productive and easy to performThis work was supported by grant AIR 3 CT 94-1921 from the European Community.Peer reviewe

Cationic Nanoparticle Interactions with Catabolic Cartilage Modify Macrophage Cytokine Production

Cationic nanoparticles (NPs) have emerged as promising candidates for intra-articular drug delivery, showcasing their potential as efficient carriers. However, despite their evident utility, many studies neglect to investigate their interactions with the extracellular matrix (ECM). This oversight represents a significant gap in our understanding, particularly in the context of osteoarthritis (OA) treatment, where viable therapeutic options are limited. The clinical translation of functionalize drug carriers for OA has been hindered, in part, by our incomplete comprehension of how these materials interact with and respond to the pathological environment. This study aims to address this gap by examining how cationic NPs interact with ECM components in an ex vivo OA cartilage explant model. By comparing the behavior of smaller (<10nm) and larger (~270 nm) cationic NPs and subjecting them to explants preconditioned with OA-specific catabolic enzymes, we observed multifaceted effects on ECM integrity and biomolecule conformation. All NP-biomolecule complexes induced differential cytokine production from stimulated macrophages. Smaller polyamidoamine (PAMAM) nanocarriers reduced glycosaminoglycan (GAG) release from explants but increased proinflammatory cytokine stimulation under pathological conditions, while larger poly lactic(co-glycolic)acid(PLGA)/polyethylenimine(PEI)-based nanocarriers maintained GAG release but induced a significantly lower proinflammatory cytokine response. Collagenase-induced OA-mimicking preconditioning produced distinct profiles across the NP panel in GAG interactions and cytokine production. The findings from these studies underscore the significance of tailored nanocarrier approaches to achieve optimal therapeutic efficacy for OA and other complex diseases