6 research outputs found

    Hydrogel nanoparticle encapsulated plasmid as a suitable gene delivery system

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    To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmid-complex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be alg/chi nanoparticles.Для облСгчСния доставки гСнСтичСского ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π° Π½Π΅ΠΎΠ±Ρ…ΠΎΠ΄ΠΈΠΌΠΎ использованиС подходящих носитСлСй, Ρ‚Π°ΠΊΠΈΡ… ΠΊΠ°ΠΊ ΠΏΠΎΠ»ΠΈΠΌΠ΅Ρ€Ρ‹. Наночастицы, состоящиС ΠΈΠ· Ρ…ΠΈΡ‚ΠΎΠ·Π°Π½-Π°Π»ΡŒΠ³ΠΈΠ½Π°Ρ‚Π½Ρ‹Ρ… ΠΏΠΎΠ»ΠΈΠΌΠ΅Ρ€ΠΎΠ², Π±Ρ‹Π»ΠΈ ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Ρ‹ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ прСгСля. Chi/Alg наночастицы ΠΈΠΌΠ΅Π»ΠΈ срСдний Π΄ΠΈΠ°ΠΌΠ΅Ρ‚Ρ€ 161.8 Π½ΠΌ (Z-Average) ΠΈ срСдний zeta-ΠΏΠΎΡ‚Π΅Π½Ρ†ΠΈΠ°Π» 29.3 mV. ΠžΡ‚ΡΡƒΡ‚ΡΡ‚Π²ΠΈΠ΅ ΠΌΠΈΠ³Ρ€Π°Ρ†ΠΈΠΈ Π”ΠΠš Π²ΠΎ врСмя элСктрофорСза комплСксов ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ‹ с наночастицами ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΎ, Ρ‡Ρ‚ΠΎ Chi/Alg наночастицы ΠΌΠΎΠ³ΡƒΡ‚ ΡƒΠ΄Π΅Ρ€ΠΆΠΈΠ²Π°Ρ‚ΡŒ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΡƒΡŽ Π”ΠΠš Π²Π½ΡƒΡ‚Ρ€ΠΈ комплСкса. Π­Ρ„Ρ„Π΅ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ наночастиц для трансфСкции ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ‹ pEGFP-N1 Π² ΠΊΡƒΠ»ΡŒΡ‚ΠΈΠ²ΠΈΡ€ΡƒΠ΅ΠΌΡ‹Π΅ ΠΊΠ»Π΅Ρ‚ΠΊΠΈ HEK 293 Π±Ρ‹Π»Π° ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½Π° с ΠΏΠΎΠΌΠΎΡ‰ΡŒΡŽ Тидкостной Ρ†ΠΈΡ‚ΠΎΠΌΠ΅Ρ‚Ρ€ΠΈΠΈ. ВСсты Π½Π° ТизнСспособ-Π½ΠΎΡΡ‚ΡŒ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, Ρ‡Ρ‚ΠΎ наночастицы Π½Π΅ ΠΈΠΌΠ΅Π»ΠΈ токсичного эффСкта Π½Π° ΠΊΠ»Π΅Ρ‚ΠΊΠΈ HEK 293 Ρ‡Π΅Ρ€Π΅Π· 4 Ρ‡ ΠΈΠ»ΠΈ 24 Ρ‡. Наночастицы Alg/Chi ΡΠ²Π»ΡΡŽΡ‚ΡΡ подходящим ΠΊΠ°Π½Π΄ΠΈΠ΄Π°Ρ‚ΠΎΠΌ для доставки Π³Π΅Π½ΠΎΠ²

    Biodiversity of rove beetles (Coleoptera: Staphylinoidea: Staphylinidae) from the Arasbaran biosphere reserve and vicinity, northwestern Iran

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    Ghahari, H., Anlaş, S., Sakenin, H., Ostovan, H., Havaskary, M. (2009): Biodiversity of rove beetles (Coleoptera: Staphylinoidea: Staphylinidae) from the Arasbaran biosphere reserve and vicinity, northwestern Iran. Linzer biologische BeitrÀge 41 (2): 1949-195

    A faunistic survey on Heteroptera (Insecta) of Western Iran

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    Sakenin, H., Samin, N., Rastegari, J., Havaskary, M., Imani, S., R.E (2010): A faunistic survey on Heteroptera (Insecta) of Western Iran. Linzer biologische BeitrΓ€ge 43 (1): 861-872, DOI: http://doi.org/10.5281/zenodo.532562

    An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands

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    Ghahari, H., Havaskary, M., Tabari, M., Ostovan, H., Sakenin, H., Satar, A. (2009): An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands. Linzer biologische BeitrΓ€ge 41 (1): 639-672, DOI: http://doi.org/10.5281/zenodo.527624
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