8 research outputs found
Hydrogel nanoparticle encapsulated plasmid as a suitable gene delivery system
To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmid-complex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be alg/chi nanoparticles.ΠΠ»Ρ ΠΎΠ±Π»Π΅Π³ΡΠ΅Π½ΠΈΡ Π΄ΠΎΡΡΠ°Π²ΠΊΠΈ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΡΡΠΈΡ
Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ, ΡΠ°ΠΊΠΈΡ
ΠΊΠ°ΠΊ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΡ. ΠΠ°Π½ΠΎΡΠ°ΡΡΠΈΡΡ, ΡΠΎΡΡΠΎΡΡΠΈΠ΅ ΠΈΠ· Ρ
ΠΈΡΠΎΠ·Π°Π½-Π°Π»ΡΠ³ΠΈΠ½Π°ΡΠ½ΡΡ
ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΎΠ², Π±ΡΠ»ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΏΡΠ΅Π³Π΅Π»Ρ. Chi/Alg Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΡ ΠΈΠΌΠ΅Π»ΠΈ ΡΡΠ΅Π΄Π½ΠΈΠΉ Π΄ΠΈΠ°ΠΌΠ΅ΡΡ 161.8 Π½ΠΌ (Z-Average) ΠΈ ΡΡΠ΅Π΄Π½ΠΈΠΉ zeta-ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» 29.3 mV. ΠΡΡΡΡΡΡΠ²ΠΈΠ΅ ΠΌΠΈΠ³ΡΠ°ΡΠΈΠΈ ΠΠΠ Π²ΠΎ Π²ΡΠ΅ΠΌΡ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·Π° ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ Ρ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΠ°ΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΎ, ΡΡΠΎ Chi/Alg Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΡ ΠΌΠΎΠ³ΡΡ ΡΠ΄Π΅ΡΠΆΠΈΠ²Π°ΡΡ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΡΡ ΠΠΠ Π²Π½ΡΡΡΠΈ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ°. ΠΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ Π΄Π»Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΠΈ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ pEGFP-N1 Π² ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΡΠ΅ΠΌΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ HEK 293 Π±ΡΠ»Π° ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½Π° Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ. Π’Π΅ΡΡΡ Π½Π° ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±-Π½ΠΎΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, ΡΡΠΎ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΡ Π½Π΅ ΠΈΠΌΠ΅Π»ΠΈ ΡΠΎΠΊΡΠΈΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ° Π½Π° ΠΊΠ»Π΅ΡΠΊΠΈ HEK 293 ΡΠ΅ΡΠ΅Π· 4 Ρ ΠΈΠ»ΠΈ 24 Ρ. ΠΠ°Π½ΠΎΡΠ°ΡΡΠΈΡΡ Alg/Chi ΡΠ²Π»ΡΡΡΡΡ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΡΡΠΈΠΌ ΠΊΠ°Π½Π΄ΠΈΠ΄Π°ΡΠΎΠΌ Π΄Π»Ρ Π΄ΠΎΡΡΠ°Π²ΠΊΠΈ Π³Π΅Π½ΠΎΠ²
Scelio flavibarbis
Scelio flavibarbis (MARSHALL 1874) M a t e r i a l: Khuzestan province: Ahwaz, 3♀♀, 13, October 2003. Comment: KHAJEHZADEH and GHAZAVI (2000) reported that this parasitoid infests 27 % of the egg capsules resulting in destruction of some 4-16 % of eggs of Locusta migratoria.Published as part of Ghahari, H., Havaskary, M., Tabari, M., Ostovan, H., Sakenin, H. & Satar, A., 2009, An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands, pp. 639-672 in Linzer biologische BeitrΓ€ge 41 (1) on pages 663-664, DOI: 10.5281/zenodo.527624
Biodiversity of rove beetles (Coleoptera: Staphylinoidea: Staphylinidae) from the Arasbaran biosphere reserve and vicinity, northwestern Iran
Ghahari, H., AnlaΕ, S., Sakenin, H., Ostovan, H., Havaskary, M. (2009): Biodiversity of rove beetles (Coleoptera: Staphylinoidea: Staphylinidae) from the Arasbaran biosphere reserve and vicinity, northwestern Iran. Linzer biologische BeitrΓ€ge 41 (2): 1949-195
Parasarcophaga jakobsoni ROHDENDORF 1937
<i>Parasarcophaga jakobsoni</i> ROHDENDORF 1937 <p>M a t e r i a l: Mazandaran province: Joibar, 13, September 2002.</p> <p> In this paper a diverse fauna of several predators and parasitoids were collected as the natural enemies of Iranian grasshoppers. In addition to this paper, many natural enemies including, <i>Blaesoxipha</i> spp. (Dip.: Sarcophagidae), <i>Stenopogon</i> spp. (Dip.: Asilidae), <i>Mylabris</i> spp. (Col.: Meloidae), <i>Sphex</i> spp. (Hym.: Sphecidae), <i>Mantis</i> sp. (Mantodea), <i>Cataglyphis</i> spp. (Formicidae), <i>Scelio flavibarbis</i> (Hym.: Scelionidae), <i>Podapolipus grissi</i> and <i>Leptus</i> sp. (Acari) and <i>Agriope</i> spp. (Aranei) were recorded by KHAJEHZADEH (2004). Also, four parasitic mites including, <i>Leptus fathipeuri</i> HAITLINGER & SABOORI, <i>L. zhangi</i> SABOORI & ATAMEHR, <i>L.</i> <i>esmailii</i> SABOORI & OSTOVAN and <i>Keramotrombium talebii</i> were reported by KARIMI IRAVANLOU & SABOORI (2000) and KARIMI IRAVANLOU & KAMALI (2000) as the parasites of Acrididae. Therefore, the populations of Orthoptera in Iranian rice fields are pressured severely by diverse natural enemies and the grasshoppers generally are not the key pests of rice fields.</p>Published as part of <i>Ghahari, H., Havaskary, M., Tabari, M., Ostovan, H., Sakenin, H. & Satar, A., 2009, An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands, pp. 639-672 in Linzer biologische BeitrΓ€ge 41 (1)</i> on page 665, DOI: <a href="http://zenodo.org/record/5276249">10.5281/zenodo.5276249</a>
A faunistic survey on Heteroptera (Insecta) of Western Iran
Sakenin, H., Samin, N., Rastegari, J., Havaskary, M., Imani, S., R.E (2010): A faunistic survey on Heteroptera (Insecta) of Western Iran. Linzer biologische BeitrΓ€ge 43 (1): 861-872, DOI: http://doi.org/10.5281/zenodo.532562
An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands
Ghahari, H., Havaskary, M., Tabari, M., Ostovan, H., Sakenin, H., Satar, A. (2009): An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands. Linzer biologische BeitrΓ€ge 41 (1): 639-672, DOI: http://doi.org/10.5281/zenodo.527624