8 research outputs found

    Hydrogel nanoparticle encapsulated plasmid as a suitable gene delivery system

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    To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmid-complex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be alg/chi nanoparticles.Для облСгчСния доставки гСнСтичСского ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π° Π½Π΅ΠΎΠ±Ρ…ΠΎΠ΄ΠΈΠΌΠΎ использованиС подходящих носитСлСй, Ρ‚Π°ΠΊΠΈΡ… ΠΊΠ°ΠΊ ΠΏΠΎΠ»ΠΈΠΌΠ΅Ρ€Ρ‹. Наночастицы, состоящиС ΠΈΠ· Ρ…ΠΈΡ‚ΠΎΠ·Π°Π½-Π°Π»ΡŒΠ³ΠΈΠ½Π°Ρ‚Π½Ρ‹Ρ… ΠΏΠΎΠ»ΠΈΠΌΠ΅Ρ€ΠΎΠ², Π±Ρ‹Π»ΠΈ ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Ρ‹ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ прСгСля. Chi/Alg наночастицы ΠΈΠΌΠ΅Π»ΠΈ срСдний Π΄ΠΈΠ°ΠΌΠ΅Ρ‚Ρ€ 161.8 Π½ΠΌ (Z-Average) ΠΈ срСдний zeta-ΠΏΠΎΡ‚Π΅Π½Ρ†ΠΈΠ°Π» 29.3 mV. ΠžΡ‚ΡΡƒΡ‚ΡΡ‚Π²ΠΈΠ΅ ΠΌΠΈΠ³Ρ€Π°Ρ†ΠΈΠΈ Π”ΠΠš Π²ΠΎ врСмя элСктрофорСза комплСксов ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ‹ с наночастицами ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΎ, Ρ‡Ρ‚ΠΎ Chi/Alg наночастицы ΠΌΠΎΠ³ΡƒΡ‚ ΡƒΠ΄Π΅Ρ€ΠΆΠΈΠ²Π°Ρ‚ΡŒ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΡƒΡŽ Π”ΠΠš Π²Π½ΡƒΡ‚Ρ€ΠΈ комплСкса. Π­Ρ„Ρ„Π΅ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ наночастиц для трансфСкции ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ‹ pEGFP-N1 Π² ΠΊΡƒΠ»ΡŒΡ‚ΠΈΠ²ΠΈΡ€ΡƒΠ΅ΠΌΡ‹Π΅ ΠΊΠ»Π΅Ρ‚ΠΊΠΈ HEK 293 Π±Ρ‹Π»Π° ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½Π° с ΠΏΠΎΠΌΠΎΡ‰ΡŒΡŽ Тидкостной Ρ†ΠΈΡ‚ΠΎΠΌΠ΅Ρ‚Ρ€ΠΈΠΈ. ВСсты Π½Π° ТизнСспособ-Π½ΠΎΡΡ‚ΡŒ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, Ρ‡Ρ‚ΠΎ наночастицы Π½Π΅ ΠΈΠΌΠ΅Π»ΠΈ токсичного эффСкта Π½Π° ΠΊΠ»Π΅Ρ‚ΠΊΠΈ HEK 293 Ρ‡Π΅Ρ€Π΅Π· 4 Ρ‡ ΠΈΠ»ΠΈ 24 Ρ‡. Наночастицы Alg/Chi ΡΠ²Π»ΡΡŽΡ‚ΡΡ подходящим ΠΊΠ°Π½Π΄ΠΈΠ΄Π°Ρ‚ΠΎΠΌ для доставки Π³Π΅Π½ΠΎΠ²

    Scelio flavibarbis

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    Scelio flavibarbis (MARSHALL 1874) M a t e r i a l: Khuzestan province: Ahwaz, 3♀♀, 13, October 2003. Comment: KHAJEHZADEH and GHAZAVI (2000) reported that this parasitoid infests 27 % of the egg capsules resulting in destruction of some 4-16 % of eggs of Locusta migratoria.Published as part of Ghahari, H., Havaskary, M., Tabari, M., Ostovan, H., Sakenin, H. & Satar, A., 2009, An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands, pp. 639-672 in Linzer biologische BeitrΓ€ge 41 (1) on pages 663-664, DOI: 10.5281/zenodo.527624

    Biodiversity of rove beetles (Coleoptera: Staphylinoidea: Staphylinidae) from the Arasbaran biosphere reserve and vicinity, northwestern Iran

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    Ghahari, H., Anlaş, S., Sakenin, H., Ostovan, H., Havaskary, M. (2009): Biodiversity of rove beetles (Coleoptera: Staphylinoidea: Staphylinidae) from the Arasbaran biosphere reserve and vicinity, northwestern Iran. Linzer biologische BeitrÀge 41 (2): 1949-195

    Parasarcophaga jakobsoni ROHDENDORF 1937

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    <i>Parasarcophaga jakobsoni</i> ROHDENDORF 1937 <p>M a t e r i a l: Mazandaran province: Joibar, 13, September 2002.</p> <p> In this paper a diverse fauna of several predators and parasitoids were collected as the natural enemies of Iranian grasshoppers. In addition to this paper, many natural enemies including, <i>Blaesoxipha</i> spp. (Dip.: Sarcophagidae), <i>Stenopogon</i> spp. (Dip.: Asilidae), <i>Mylabris</i> spp. (Col.: Meloidae), <i>Sphex</i> spp. (Hym.: Sphecidae), <i>Mantis</i> sp. (Mantodea), <i>Cataglyphis</i> spp. (Formicidae), <i>Scelio flavibarbis</i> (Hym.: Scelionidae), <i>Podapolipus grissi</i> and <i>Leptus</i> sp. (Acari) and <i>Agriope</i> spp. (Aranei) were recorded by KHAJEHZADEH (2004). Also, four parasitic mites including, <i>Leptus fathipeuri</i> HAITLINGER & SABOORI, <i>L. zhangi</i> SABOORI & ATAMEHR, <i>L.</i> <i>esmailii</i> SABOORI & OSTOVAN and <i>Keramotrombium talebii</i> were reported by KARIMI IRAVANLOU & SABOORI (2000) and KARIMI IRAVANLOU & KAMALI (2000) as the parasites of Acrididae. Therefore, the populations of Orthoptera in Iranian rice fields are pressured severely by diverse natural enemies and the grasshoppers generally are not the key pests of rice fields.</p>Published as part of <i>Ghahari, H., Havaskary, M., Tabari, M., Ostovan, H., Sakenin, H. & Satar, A., 2009, An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands, pp. 639-672 in Linzer biologische BeitrΓ€ge 41 (1)</i> on page 665, DOI: <a href="http://zenodo.org/record/5276249">10.5281/zenodo.5276249</a&gt

    A faunistic survey on Heteroptera (Insecta) of Western Iran

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    Sakenin, H., Samin, N., Rastegari, J., Havaskary, M., Imani, S., R.E (2010): A faunistic survey on Heteroptera (Insecta) of Western Iran. Linzer biologische BeitrΓ€ge 43 (1): 861-872, DOI: http://doi.org/10.5281/zenodo.532562

    An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands

    No full text
    Ghahari, H., Havaskary, M., Tabari, M., Ostovan, H., Sakenin, H., Satar, A. (2009): An annotated catalogue of Orthoptera (Insecta) and their natural enemies from Iranian rice fields and surrounding grasslands. Linzer biologische BeitrΓ€ge 41 (1): 639-672, DOI: http://doi.org/10.5281/zenodo.527624
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