Tiny Microbes, Big Data:Gut Microbiota Profiling for Clinical Diagnostics

Savelkoul, P.H.M. [Promotor]Bodegraven, A.A. van [Copromotor]Budding, A.E. [Copromotor

High-Q Gold and Silicon Nitride Bilayer Nanostrings

Low-mass, high-Q, silicon nitride nanostrings are at the cutting edge of nanomechanical devices for sensing applications. Here we show that the addition of a chemically functionalizable gold overlayer does not adversely affect the Q of the fundamental out-of-plane mode. Instead the device retains its mechanical responsiveness while gaining sensitivity to molecular bonding. Furthermore, differences in thermal expansion within the bilayer give rise to internal stresses that can be electrically controlled. In particular, an alternating current excites resonant motion of the nanostring. This AC thermoelastic actuation is simple, robust, and provides an integrated approach to sensor actuation.Comment: 5 pages, 4 figures + supplementary materia

Nanosecond surface interferometry measurements on designed and commercial polymers

The effect of the ablation mechanism on surface morphology changes during an ablation process was studied by comparing three different polymers: a triazene polymer, a polyimide and poly(methylmethacrylate) (PMMA) with nanosecond surface interferometry. The triazene polymer, for which only indications for a photochemical ablation mechanism had been detected in previous studies, revealed no surface swelling, which could be attributed to a thermal ablation mechanism. For polyimide, a photothermal ablation mechanism is usually used to describe the ablation process at irradiation wavelengths ≥248nm. However, the interferometric measurements do not show any surface swelling, which would be a clear indication for a thermal ablation mechanism. A surface swelling was only detected for PMMA with irradiation at 248nm and fluences below the threshold of permanent surface modification. The detected phase shift, which is proportional to the change of the film thickness and the refractive index, can be explained by the opposite signs of the thermal expansion coefficient and the thermal refractive-index coefficien

Enhanced Anandamide Plasma Levels in Patients with Complex Regional Pain Syndrome following Traumatic Injury: A Preliminary Report

The complex regional pain syndrome (CRPS) is a disabling neuropathic pain condition that may develop following injuries of the extremities. The pathogenesis of this syndrome is not clear; however, it includes complex interactions between the nervous and the immune system resulting in chronic inflammation, pain and trophic changes. This interaction may be mediated by chronic stress which is thought to activate the endogenous cannabinoid (endocannabinoid) system (ECS). We conducted an open, prospective, comparative clinical study to determine plasma level of the endocannabinoid anandamide by high-performance liquid chromatography and a tandem mass spectrometry system in 10 patients with CRPS type I versus 10 age- and sex-matched healthy controls. As compared to healthy controls, CRPS patients showed significantly higher plasma concentrations of anandamide. These results indicate that the peripheral ECS is activated in CRPS. Further studies are warranted to evaluate the role of the ECS in the limitation of inflammation and pain. Copyright (C) 2009 S. Karger AG, Base

Quiver Theories from D6-branes via Mirror Symmetry

We study N=1 four dimensional quiver theories arising on the worldvolume of D3-branes at del Pezzo singularities of Calabi-Yau threefolds. We argue that under local mirror symmetry D3-branes become D6-branes wrapped on a three torus in the mirror manifold. The type IIB (p,q) 5-brane web description of the local del Pezzo, being closely related to the geometry of its mirror manifold, encodes the geometry of 3-cycles and is used to obtain gauge groups, quiver diagrams and the charges of the fractional branes.Comment: 30 pages, citations adde

Phonon quantum nondemolition measurements in nonlinearly coupled optomechanical cavities

In the field of cavity optomechanics, proposals for quantum nondemolition (QND) measurements of phonon number provide a promising avenue by which one can study the quantum nature of nanoscale mechanical resonators. Here we investigate these QND measurements for an optomechanical system whereby quadratic coupling arises due to shared symmetries between a single optical resonance and a mechanical mode. We establish a relaxed limit on the amount of linear coupling that can exist in this type of system while still allowing for QND measurements of mechanical Fock states. The ability to perform optomechanical QND measurements of this nature would allow one to probe the decoherence of these mesoscopic states, providing an experimental test bed for quantum collapse theories